中国药师
中國藥師
중국약사
CHINA PHARMACIST
2015年
4期
582-584
,共3页
亲水作用色谱%蒸发光散射检测%山药%尿囊素
親水作用色譜%蒸髮光散射檢測%山藥%尿囊素
친수작용색보%증발광산사검측%산약%뇨낭소
HILIC%ELSD%Rhizoma Dioscoreae%Allantoin
目的:建立山药中尿囊素的HILIC-HPLC-ELSD含量测定方法。方法:色谱柱为Waters XBridge HILIC(150 mm ×4.6 mm,5μm);流动相为乙腈-水(85:15);流速为0.8 ml·min-1;雾化压力:40 psi;漂移管温度:80℃;雾化器温度:50℃。结果:尿囊素在1.5~7.5μg范围内线性关系良好(r=0.9981),线性方程为Y=1.67X+2.35,检出限为25 ng,平均回收率为99.9%,RSD=0.72%(n=9)。结论:该方法简单、可靠,可用于山药中尿囊素的质量控制。
目的:建立山藥中尿囊素的HILIC-HPLC-ELSD含量測定方法。方法:色譜柱為Waters XBridge HILIC(150 mm ×4.6 mm,5μm);流動相為乙腈-水(85:15);流速為0.8 ml·min-1;霧化壓力:40 psi;漂移管溫度:80℃;霧化器溫度:50℃。結果:尿囊素在1.5~7.5μg範圍內線性關繫良好(r=0.9981),線性方程為Y=1.67X+2.35,檢齣限為25 ng,平均迴收率為99.9%,RSD=0.72%(n=9)。結論:該方法簡單、可靠,可用于山藥中尿囊素的質量控製。
목적:건립산약중뇨낭소적HILIC-HPLC-ELSD함량측정방법。방법:색보주위Waters XBridge HILIC(150 mm ×4.6 mm,5μm);류동상위을정-수(85:15);류속위0.8 ml·min-1;무화압력:40 psi;표이관온도:80℃;무화기온도:50℃。결과:뇨낭소재1.5~7.5μg범위내선성관계량호(r=0.9981),선성방정위Y=1.67X+2.35,검출한위25 ng,평균회수솔위99.9%,RSD=0.72%(n=9)。결론:해방법간단、가고,가용우산약중뇨낭소적질량공제。
Objective:To establish an HILIC-HPLC-ELSD method for the determination of allantoin in Rhizoma Dioscoreae. Methods:The separation was performed on a Waters XBridge HILIC column(150 mm × 4. 6 mm,5μm),the mobile phase was ace-tonitrile-water(85:15)with the flow rate of 0. 8 ml·min-1 ,and the nebulizer pressure was 40psi,the drifting tube temperature was 80℃ and the nebulizer temperature was 50℃. Results:The linearity of allantoin was good within the range of 1. 5-7. 5 μg( r =0. 998 1). The regress equation was Y=1. 67X+2. 35,the detection limit was 25 ng,and the average recovery of allantoin in Rhizoma Dioscoreae was 99. 9% with RSD of 0. 72%(n=9). Conclusion:The method is simple and reliable,which can be used in the deter-mination of allantoin in Rhizoma Dioscoreae.