CAJ | 학술논문

采用毒性标准实验方法研究了Cd2＋与S-异丙甲草胺单独及联合作用对斜生栅藻急性毒性、总可溶性蛋白含量、超氧化物歧化酶( SOD)活性和细胞膜通透性的影响.结果表明Cd2＋与S-异丙甲草胺单独作用时EC50均随时间的延长而减小,且S-异丙甲草胺的急性毒性大于Cd2＋； Cd2＋、 S-异丙甲草胺的EC50-24h分别为0.27 mg·L-1、0.24 mg·L-1,EC50-96h分别为0.16 mg·L-1、0.13 mg·L-1. Cd2＋与S-异丙甲草胺联合作用时低浓度表现为协同作用,高浓度表现为拮抗作用.暴露96 h后,Cd2＋与S-异丙甲草胺单独及联合作用下,随有毒物质浓度升高,斜生栅藻总可溶性蛋白含量降低,SOD酶活性先激活后抑制,细胞膜通透性逐渐增大.
채용독성표준실험방법연구료Cd2＋여S-이병갑초알단독급연합작용대사생책조급성독성、총가용성단백함량、초양화물기화매( SOD)활성화세포막통투성적영향.결과표명Cd2＋여S-이병갑초알단독작용시EC50균수시간적연장이감소,차S-이병갑초알적급성독성대우Cd2＋； Cd2＋、 S-이병갑초알적EC50-24h분별위0.27 mg·L-1、0.24 mg·L-1,EC50-96h분별위0.16 mg·L-1、0.13 mg·L-1. Cd2＋여S-이병갑초알연합작용시저농도표현위협동작용,고농도표현위길항작용.폭로96 h후,Cd2＋여S-이병갑초알단독급연합작용하,수유독물질농도승고,사생책조총가용성단백함량강저,SOD매활성선격활후억제,세포막통투성축점증대.
The single and combined effects of Cd2+ and S-metolachlor on acute toxicity, total soluble protein content, superoxide dismutase ( SOD) activity and cell membrane permeability of Scenedesmus obliquus ( S. obliquus) were studied using the standard toxic testing methods. The results showed that the EC50 of Cd2+ and S-metolachlor decreased with time, and the acute toxicity of S-metolachlor was higher than that of Cd2+, EC50-24h of Cd2+and S-metolachlor was 0. 27 mg·L-1 and 0. 24 mg·L-1 , respectively, and EC50-96h was 0. 16 mg·L-1 and 0. 13 mg·L-1 , respectively. The combined toxicity of Cd2+ and S-metolachlor showed a synergistic effects at low concentration, and antagonism effects at high concentration. After 96h-exposure, the total soluble protein content of S. obliquus decreased, the SOD activity first increased and then decreased, and the cell membrane permeability increased with the increasing concentration of both single and combined treatment.