CAJ | 학술논문

目的：构建稳定、可行、可重复的兔慢性肝损伤胆石症模型。方法将90只新西兰大白兔随机分为空白对照组、肝纤维化组、肝纤维化胆石症组、肝硬化组和肝硬化胆石症组,空白对照组10只,其余各组各20只。空白对照组正常饮食,肝纤维化组、肝纤维化胆石症组予20%的 CCl4-橄榄油溶液0.2 ml/kg 腹腔注射,肝硬化组、肝硬化胆石症组予25%的 CCl4-橄榄油溶液0.3 ml/kg 腹腔注射,每4 d 1次,连续12周,进行肝组织病理学检查及 Masson 染色,计算结石形成率。结果肝纤维化组、肝硬化组、肝纤维化胆石症组和肝硬化胆石症组结石形成率分别为5%、10%、70%、70%,空白对照组、肝纤维化组与肝纤维化胆石症组成石率的三组间比较差异具有统计学意义(χ2=25.476,P <0.001),两两比较结果显示,空白对照组、肝纤维化组与肝纤维化胆石症组的比较差异均具有统计学意义(P 1<0.001；P 2<0.001)；空白对照组、肝硬化组与肝硬化胆石症组成石率的三组间比较差异具有统计学意义(χ2=22.426,P <0.001),两两比较结果显示,空白对照组、肝硬化组与肝硬化胆石症组的比较差异均具有统计学意义(P 1<0.001；P 2<0.001)。结石主要为胆固醇结石,HE 染色光镜下可见肝小叶结构遭到破坏,肝细胞肿大；Masson 染色见纤维组织增多并变为蓝色,尤其在肝小叶汇管区较为明显,可见假小叶。结论通过用 CCl4和高胆固醇、高热量致石饲料,能成功构建稳定、可行的兔慢性肝损伤胆石症模型,为慢性肝损伤兔胆石症实验动物模型的构建提供一定的借鉴和指导意义。
목적：구건은정、가행、가중복적토만성간손상담석증모형。방법장90지신서란대백토수궤분위공백대조조、간섬유화조、간섬유화담석증조、간경화조화간경화담석증조,공백대조조10지,기여각조각20지。공백대조조정상음식,간섬유화조、간섬유화담석증조여20%적 CCl4-감람유용액0.2 ml/kg 복강주사,간경화조、간경화담석증조여25%적 CCl4-감람유용액0.3 ml/kg 복강주사,매4 d 1차,련속12주,진행간조직병이학검사급 Masson 염색,계산결석형성솔。결과간섬유화조、간경화조、간섬유화담석증조화간경화담석증조결석형성솔분별위5%、10%、70%、70%,공백대조조、간섬유화조여간섬유화담석증조성석솔적삼조간비교차이구유통계학의의(χ2=25.476,P <0.001),량량비교결과현시,공백대조조、간섬유화조여간섬유화담석증조적비교차이균구유통계학의의(P 1<0.001；P 2<0.001)；공백대조조、간경화조여간경화담석증조성석솔적삼조간비교차이구유통계학의의(χ2=22.426,P <0.001),량량비교결과현시,공백대조조、간경화조여간경화담석증조적비교차이균구유통계학의의(P 1<0.001；P 2<0.001)。결석주요위담고순결석,HE 염색광경하가견간소협결구조도파배,간세포종대；Masson 염색견섬유조직증다병변위람색,우기재간소협회관구교위명현,가견가소협。결론통과용 CCl4화고담고순、고열량치석사료,능성공구건은정、가행적토만성간손상담석증모형,위만성간손상토담석증실험동물모형적구건제공일정적차감화지도의의。
Objective To establish a stable,feasible and repetitive rabbit cholelithiasis model induced by chronic liver injury. Methods Ninety New Zealand white rabbits were randomly divided into 5 groups:a blank control group (n =10),a liver fibrosis group(n =20),liver fibrosis cholelithiasis group(n =20),a liv-er cirrhosis group(n =20)and a liver cirrhosis cholelithiasis group(n =20).Among these groups,the rabbits in the blank control group was fed with normal diet,however,the rabbits of the liver fibrosis group and the liver fibrosis cholelithiasis group received 20% CCl4 - olive oil solution by intraperitoneal injection of 0.2 ml/kg,and the rabbits of the liver cirrhosis group and the liver cirrhosis cholelithiasis group received 25% CCl4 -olive oil solution by intraperitoneal injection of 0.3 ml/kg,once every 4 days for 12 weeks consecutively;then the formation rate of stone was calculated and the liver tissue histopathology examination and Masson staining were performed. Results The stone formation rates of the liver fibrosis group,of the liver cirrhosis group, of the liver fibrosis cholelithiasis group,and the liver cirrhosis cholelithiasis group was 5%,10%,70% and 70%,respectively.Tri-group comparison of stone formation rate among the blank control group,the liver fi-brosis group and the liver fibrosis cholelithiasis group yielded statistically difference (χ2 =25.476,P <0.001). Group-paired comparison of stone formation rate among the blank control group,the liver fibrosis group and the liver fibrosis cholelithiasis group yielded statistically difference (P 1 < 0.001,P 2 < 0.001 );tri - group comparison of stone formation rate among the blank control group,the liver cirrhosis group and the liver cir-rhosis cholelithiasis group yielded statistically difference (χ2 =22.426,P <0.001),group-paired comparison of stone formation rate among the blank control group,the liver cirrhosis group and the liver cirrhosis choleli-thiasis group yielded statistically difference (P 1 <0.001,P 2 <0.001).The results mainly presented as choles-terol stones,the structure of visible hepatic lobule by HE staining was destroyed,swelling liver cell;Masson staining showed that fibrous tissues increased and became blue,especially in the liver portal area,and the visi-ble false lobule. Conclusion Use of CCl4 combined the high cholesterol and lithogenic diet with high quantity of heat can build a stable,feasible,repetitive cholelithiasis rabbit model caused by chronic liver injury,which will provide reference and guidance for constructing experimental animal cholelithiasis models in rabbits with chronic liver injury.