中国农业科学
中國農業科學
중국농업과학
SCIENTIA AGRICULTURA SINICA
2015年
9期
1825-1835
,共11页
王宝维%陈苗璐%王秉翰%张名爱%葛文华%程凡升%岳斌
王寶維%陳苗璐%王秉翰%張名愛%葛文華%程凡升%嶽斌
왕보유%진묘로%왕병한%장명애%갈문화%정범승%악빈
锌%鹅%免疫%抗氧化能力%金属硫蛋白-Ⅰ表达量%相关性
鋅%鵝%免疫%抗氧化能力%金屬硫蛋白-Ⅰ錶達量%相關性
자%아%면역%항양화능력%금속류단백-Ⅰ표체량%상관성
zinc%geese%immune%antioxidant capacity%MT-I mRNA gene%correlation analysis
【目的】研究不同水平锌对5—15周龄鹅免疫抗氧化功能与金属硫蛋白-Ⅰ（MT-I ）mRNA表达量的影响及相互关系，以科学地确定鹅饲粮中锌的需要量。【方法】试验随机选用29日龄体重相近的青农灰鹅360只（P＜0.05），设计6个处理，每处理6次重复，每重复10只（公母各半）；各处理饲粮中锌水平分别为：27.46（Ⅰ组）、77.46（Ⅱ组）、127.46（Ⅲ组）、177.46（Ⅳ组）、227.46（Ⅴ组）、277.46（Ⅵ组）mg·kg-1（基础饲粮中含锌），试验进行11周。【结果】(1)胸腺指数、脾脏指数和法氏囊指数受饲粮中锌水平的影响，随锌水平的升高呈现先升高后降低趋势；当锌水平为127.46 mg·kg-1时，胸腺指数最高（P＜0.05）；当锌水平为77.46 mg·kg-1时，脾脏指数、法氏囊指数最高（P＜0.05）。(2)饲粮锌水平为177.46 mg·kg-1时，鹅副黏病毒疫苗免疫后7日的抗体滴度最高（P＜0.05）；饲粮锌水平为157.41 mg·kg-1时，鹅副黏病毒疫苗免疫后14日的抗体滴度最高（P＜0.05）。(3)当饲粮锌水平为153.84 mg·kg-1时，鹅血清和肝脏总抗氧化能力（T-AOC）活性最高（P＜0.01）；当饲粮中含锌127.46 mg·kg-1时，血清和肝脏过氧化氢酶（CAT）活性最高（P＜0.01）；当饲粮锌水平为148.33 mg·kg-1时，鹅血清和肝脏谷胱甘肽过氧化物酶（GSH-Px）活性最高（P＜0.05）；当饲粮中含锌148.33 mg·kg-1时，血清和肝脏铜锌超氧化物歧化酶（CuZn-SOD）活性最高（P＜0.01）；当饲粮中锌水平为177.46 mg·kg-1时，血清和肝脏中丙二醛（MDA）最低（P＜0.05）。(4)饲粮中锌能显著提高MT-I mRNA表达量（P＜0.05），当饲粮中锌水平为177.46 mg·kg-1时，肝脏MT-I mRNA表达量最高（P＜0.01）。(5)肝脏中MT-I mRNA表达量与肝脏中T-AOC（r=0.94）、CuZn-SOD（r=0.97）活性指标呈极显著性正相关（P＜0.01），与CAT（r=0.85）、GSH-Px（r=0.89）活性呈显著正相关（P＜0.05），与MDA呈负相关（r=-0.70,P＞0.05）；肝脏中MT-I mRNA表达量与血清中T-AOC （r=0.99）、CAT（r=0.92）和CuZn-SOD（r=0.94）活性均呈极显著正相关（P＜0.01），与GSH-Px呈正相关（r=0.81,P＞0.05），与MDA呈负相关（r=-0.39,P＞0.05）。【结论】(1)饲粮中适宜水平锌能够促进鹅免疫器官发育，增强T-AOC、CAT、GSH-Px、CuZn-SOD抗氧化酶活性，降低MDA水平。(2)饲粮中锌能够干预肝脏MT-I mRNA表达量，从而调节机体抗氧化能力。(3)鹅饲粮中锌水平在77—150 mg·kg-1范围内机体处于高位免疫和抗氧化状态。
【目的】研究不同水平鋅對5—15週齡鵝免疫抗氧化功能與金屬硫蛋白-Ⅰ（MT-I ）mRNA錶達量的影響及相互關繫，以科學地確定鵝飼糧中鋅的需要量。【方法】試驗隨機選用29日齡體重相近的青農灰鵝360隻（P＜0.05），設計6箇處理，每處理6次重複，每重複10隻（公母各半）；各處理飼糧中鋅水平分彆為：27.46（Ⅰ組）、77.46（Ⅱ組）、127.46（Ⅲ組）、177.46（Ⅳ組）、227.46（Ⅴ組）、277.46（Ⅵ組）mg·kg-1（基礎飼糧中含鋅），試驗進行11週。【結果】(1)胸腺指數、脾髒指數和法氏囊指數受飼糧中鋅水平的影響，隨鋅水平的升高呈現先升高後降低趨勢；噹鋅水平為127.46 mg·kg-1時，胸腺指數最高（P＜0.05）；噹鋅水平為77.46 mg·kg-1時，脾髒指數、法氏囊指數最高（P＜0.05）。(2)飼糧鋅水平為177.46 mg·kg-1時，鵝副黏病毒疫苗免疫後7日的抗體滴度最高（P＜0.05）；飼糧鋅水平為157.41 mg·kg-1時，鵝副黏病毒疫苗免疫後14日的抗體滴度最高（P＜0.05）。(3)噹飼糧鋅水平為153.84 mg·kg-1時，鵝血清和肝髒總抗氧化能力（T-AOC）活性最高（P＜0.01）；噹飼糧中含鋅127.46 mg·kg-1時，血清和肝髒過氧化氫酶（CAT）活性最高（P＜0.01）；噹飼糧鋅水平為148.33 mg·kg-1時，鵝血清和肝髒穀胱甘肽過氧化物酶（GSH-Px）活性最高（P＜0.05）；噹飼糧中含鋅148.33 mg·kg-1時，血清和肝髒銅鋅超氧化物歧化酶（CuZn-SOD）活性最高（P＜0.01）；噹飼糧中鋅水平為177.46 mg·kg-1時，血清和肝髒中丙二醛（MDA）最低（P＜0.05）。(4)飼糧中鋅能顯著提高MT-I mRNA錶達量（P＜0.05），噹飼糧中鋅水平為177.46 mg·kg-1時，肝髒MT-I mRNA錶達量最高（P＜0.01）。(5)肝髒中MT-I mRNA錶達量與肝髒中T-AOC（r=0.94）、CuZn-SOD（r=0.97）活性指標呈極顯著性正相關（P＜0.01），與CAT（r=0.85）、GSH-Px（r=0.89）活性呈顯著正相關（P＜0.05），與MDA呈負相關（r=-0.70,P＞0.05）；肝髒中MT-I mRNA錶達量與血清中T-AOC （r=0.99）、CAT（r=0.92）和CuZn-SOD（r=0.94）活性均呈極顯著正相關（P＜0.01），與GSH-Px呈正相關（r=0.81,P＞0.05），與MDA呈負相關（r=-0.39,P＞0.05）。【結論】(1)飼糧中適宜水平鋅能夠促進鵝免疫器官髮育，增彊T-AOC、CAT、GSH-Px、CuZn-SOD抗氧化酶活性，降低MDA水平。(2)飼糧中鋅能夠榦預肝髒MT-I mRNA錶達量，從而調節機體抗氧化能力。(3)鵝飼糧中鋅水平在77—150 mg·kg-1範圍內機體處于高位免疫和抗氧化狀態。
【목적】연구불동수평자대5—15주령아면역항양화공능여금속류단백-Ⅰ（MT-I ）mRNA표체량적영향급상호관계，이과학지학정아사량중자적수요량。【방법】시험수궤선용29일령체중상근적청농회아360지（P＜0.05），설계6개처리，매처리6차중복，매중복10지（공모각반）；각처리사량중자수평분별위：27.46（Ⅰ조）、77.46（Ⅱ조）、127.46（Ⅲ조）、177.46（Ⅳ조）、227.46（Ⅴ조）、277.46（Ⅵ조）mg·kg-1（기출사량중함자），시험진행11주。【결과】(1)흉선지수、비장지수화법씨낭지수수사량중자수평적영향，수자수평적승고정현선승고후강저추세；당자수평위127.46 mg·kg-1시，흉선지수최고（P＜0.05）；당자수평위77.46 mg·kg-1시，비장지수、법씨낭지수최고（P＜0.05）。(2)사량자수평위177.46 mg·kg-1시，아부점병독역묘면역후7일적항체적도최고（P＜0.05）；사량자수평위157.41 mg·kg-1시，아부점병독역묘면역후14일적항체적도최고（P＜0.05）。(3)당사량자수평위153.84 mg·kg-1시，아혈청화간장총항양화능력（T-AOC）활성최고（P＜0.01）；당사량중함자127.46 mg·kg-1시，혈청화간장과양화경매（CAT）활성최고（P＜0.01）；당사량자수평위148.33 mg·kg-1시，아혈청화간장곡광감태과양화물매（GSH-Px）활성최고（P＜0.05）；당사량중함자148.33 mg·kg-1시，혈청화간장동자초양화물기화매（CuZn-SOD）활성최고（P＜0.01）；당사량중자수평위177.46 mg·kg-1시，혈청화간장중병이철（MDA）최저（P＜0.05）。(4)사량중자능현저제고MT-I mRNA표체량（P＜0.05），당사량중자수평위177.46 mg·kg-1시，간장MT-I mRNA표체량최고（P＜0.01）。(5)간장중MT-I mRNA표체량여간장중T-AOC（r=0.94）、CuZn-SOD（r=0.97）활성지표정겁현저성정상관（P＜0.01），여CAT（r=0.85）、GSH-Px（r=0.89）활성정현저정상관（P＜0.05），여MDA정부상관（r=-0.70,P＞0.05）；간장중MT-I mRNA표체량여혈청중T-AOC （r=0.99）、CAT（r=0.92）화CuZn-SOD（r=0.94）활성균정겁현저정상관（P＜0.01），여GSH-Px정정상관（r=0.81,P＞0.05），여MDA정부상관（r=-0.39,P＞0.05）。【결론】(1)사량중괄의수평자능구촉진아면역기관발육，증강T-AOC、CAT、GSH-Px、CuZn-SOD항양화매활성，강저MDA수평。(2)사량중자능구간예간장MT-I mRNA표체량，종이조절궤체항양화능력。(3)아사량중자수평재77—150 mg·kg-1범위내궤체처우고위면역화항양화상태。
Objective] This experiment was conducted to determine the required level of zinc in goose feed. The effects of different levels of dietary zinc on immunity, antioxidant capacity and metallothionein (MT-I) mRNA gene expression to 5-15-week-old Qing-Nong-Hui geese were evaluated.[Method] Three hundred and sixty 29-day-old Qing-Nong-Hui geese were randomly selected and divided into six treatment groups with six replicates for each group, and each replicate contained ten geese (5♂+5♀). The content of added dietary zinc in each group was 27.46 (GroupⅠ, control), 77.46 (GroupⅡ), 127.46 (GroupⅢ), 177.46 (GroupⅣ), 227.46 (GroupⅤ), and 277.46 (GroupⅥ) mg·kg-1, respectively. The geese were fed for 11 weeks.[Result]The results were as follows: Index of thymus, spleen and bursa of fabricius showed an increase and downward trend with the effect of zinc level in feed. The thymus index obtained the highest (P<0.05) level when the added zinc in feed was 127.46 mg·kg-1, while the spleen index and the fabricius bursa index reached the highest level (P<0.05) when the added zinc in feed was 77.46 mg·kg-1. The index of immune organs decreased as long as the dietary zinc in feed was out of the range of 77.46-127.46 mg·kg-1. The antibody titer reached the highest (P<0.05) after the geese were immunized by goose paramyxovirus vaccine for 14 days when the zinc level in feed was 157.41 mg·kg-1. The activity level of total antioxidant capacity (T-AOC) for the goose serum and liver reached the highest (P<0.01) when the zinc level in feed was 153.84 mg·kg-1. The activity level of both catalase (CAT) and glutathione peroxidase (GSH-Px) for the serum and liver of goose was the highest (P<0.01) when the zinc level in feed was 148.33 mg·kg-1. The activity level of superoxide dismutase (CuZn-SOD) was the highest (P<0.01) in the goose’s serum and liver when the zinc level in feed was 148.33 mg·kg-1. On the other hand, when the zinc level in feed was 177.46 mg·kg-1, the malonaldehyde (MDA) in the serum and liver had the lowest level (P<0.05); With the increase of the zinc level in feed, the expression quantity of MT-I mRNA was significantly increased (P<0.05). And when the zinc level in the feed was 177.46 mg·kg-1, the expression quantity of MT-I mRNA of liver was the highest. It was highly significant that the expression quantity of MT-I mRNA in goose liver was positively correlated with the activity level of T-AOC (r= 0.94,P<0.01) and CuZn-SOD (r= 0.97,P<0.01). The MT-I mRNA expression level in goose liver was also positively correlated with the activity level of CAT (r=0.85,P<0.05), GSH-Px (r=0.89,P<0.05), and negatively correlated with MDA (r=-0.70,P>0.05). The expression level of MT-I mRNA in goose liver was significantly positively correlated with activity levels of T-AOC (r=0.99, P<0.01), CAT (r=0.92,P<0.01) and CuZn-SOD (r=0.94,P<0.01) in serum. It was also positively correlated with GSH-Px (r=0.81,P>0.05) and negatively correlated with MDA (r=-0.39, P>0.05).[Conclusion] Appropriate additional level of dietary zinc promoted the immune organs development and antioxidant capacity of goose. Dietary zinc also intervened the MT-I mRNA expression level, which was closely related with the body’s antioxidant capacity. Feed zinc levels in the 77-150 mg·kg-1 range can keep the body in high immunity and antioxidant capacity level of the 5-15 weeks old geese.
