浙江预防医学
浙江預防醫學
절강예방의학
ZHEJIANG JOURNAL OF PREVENTIVE MEDICINE
2015年
5期
445-449
,共5页
黄酒%动脉粥样硬化%内皮细胞%一氧化氮合酶%肿瘤坏死因子 -α
黃酒%動脈粥樣硬化%內皮細胞%一氧化氮閤酶%腫瘤壞死因子 -α
황주%동맥죽양경화%내피세포%일양화담합매%종류배사인자 -α
Yellow wine%Atherosclerosis%Endothelial cells%NOS%TNF -α
目的：观测黄酒对 TNF －α诱导的大鼠血管内皮细胞的作用，分析其对动脉粥样硬化的影响。方法大鼠原代主动脉 VECs 经分离培养及纯化鉴定后，取第3～4代细胞用于实验。根据前期实验，分为对照组、TNF －α组、TNF －α＋瑞舒伐他汀组（10μmol／L）、TNF －α＋酒精组（0．5％、1．0％、1．5％）、TNF －α＋黄酒组（0．5％、1．0％、1．5％），共有5种不同处理的9个水平组。培养24 h 后收集样品，硝酸还原酶法测定培养液上清液 NO 的含量；培养48 h 后用免疫印迹法检测 eNOS 和 iNOS 蛋白的表达量。结果与 TNF －α组相比，瑞舒伐他汀组、黄酒1．0％组、黄酒1．5％组内皮上清中 NO 含量升高，eNOS 活性增加，eNOS 蛋白的表达升高， iNOS 蛋白表达降低（P ＜0．05）；与瑞舒伐他汀组相比，黄酒1．0％组和黄酒1．5％组的 eNOS 蛋白表达降低， iNOS 蛋白表达升高（P ＜0．05）；相比酒精组（1．0％或1．5％），其对应酒精度的黄酒中 eNOS 蛋白表达升高（P ＜0．05），iNOS 蛋白表达降低（P ＜0．05）。结论小剂量黄酒能够使上清 NO 含量增加，增强 eNOS 的活性及其蛋白表达，抑制 iNOS 蛋白表达，具有类他汀样作用，这可能是其抗动脉粥样硬化的机制之一。
目的：觀測黃酒對 TNF －α誘導的大鼠血管內皮細胞的作用，分析其對動脈粥樣硬化的影響。方法大鼠原代主動脈 VECs 經分離培養及純化鑒定後，取第3～4代細胞用于實驗。根據前期實驗，分為對照組、TNF －α組、TNF －α＋瑞舒伐他汀組（10μmol／L）、TNF －α＋酒精組（0．5％、1．0％、1．5％）、TNF －α＋黃酒組（0．5％、1．0％、1．5％），共有5種不同處理的9箇水平組。培養24 h 後收集樣品，硝痠還原酶法測定培養液上清液 NO 的含量；培養48 h 後用免疫印跡法檢測 eNOS 和 iNOS 蛋白的錶達量。結果與 TNF －α組相比，瑞舒伐他汀組、黃酒1．0％組、黃酒1．5％組內皮上清中 NO 含量升高，eNOS 活性增加，eNOS 蛋白的錶達升高， iNOS 蛋白錶達降低（P ＜0．05）；與瑞舒伐他汀組相比，黃酒1．0％組和黃酒1．5％組的 eNOS 蛋白錶達降低， iNOS 蛋白錶達升高（P ＜0．05）；相比酒精組（1．0％或1．5％），其對應酒精度的黃酒中 eNOS 蛋白錶達升高（P ＜0．05），iNOS 蛋白錶達降低（P ＜0．05）。結論小劑量黃酒能夠使上清 NO 含量增加，增彊 eNOS 的活性及其蛋白錶達，抑製 iNOS 蛋白錶達，具有類他汀樣作用，這可能是其抗動脈粥樣硬化的機製之一。
목적：관측황주대 TNF －α유도적대서혈관내피세포적작용，분석기대동맥죽양경화적영향。방법대서원대주동맥 VECs 경분리배양급순화감정후，취제3～4대세포용우실험。근거전기실험，분위대조조、TNF －α조、TNF －α＋서서벌타정조（10μmol／L）、TNF －α＋주정조（0．5％、1．0％、1．5％）、TNF －α＋황주조（0．5％、1．0％、1．5％），공유5충불동처리적9개수평조。배양24 h 후수집양품，초산환원매법측정배양액상청액 NO 적함량；배양48 h 후용면역인적법검측 eNOS 화 iNOS 단백적표체량。결과여 TNF －α조상비，서서벌타정조、황주1．0％조、황주1．5％조내피상청중 NO 함량승고，eNOS 활성증가，eNOS 단백적표체승고， iNOS 단백표체강저（P ＜0．05）；여서서벌타정조상비，황주1．0％조화황주1．5％조적 eNOS 단백표체강저， iNOS 단백표체승고（P ＜0．05）；상비주정조（1．0％혹1．5％），기대응주정도적황주중 eNOS 단백표체승고（P ＜0．05），iNOS 단백표체강저（P ＜0．05）。결론소제량황주능구사상청 NO 함량증가，증강 eNOS 적활성급기단백표체，억제 iNOS 단백표체，구유류타정양작용，저가능시기항동맥죽양경화적궤제지일。
Objective To determine similarities in effect of yellow wine as compared statin and the possibility that yellow wine inhibits TNF -α-induced NO production,eNOS activity,expression of eNOS and iNOS protein in cultured rat VECs.Methods Isolation,cultivation,purification and identification of vascular endothelial cells of rat thoracic aorta in vitro were conducted.The passages 3 or 4 of VECs were used in all studies.Then we divided cells into 9 groups according to assays before:control,TNF -α,TNF -α+rosuvastatin (10 umol/L),TNF -α+ethanol 0.5%,TNF -α+yellow wine 0.5%,TNF -α+ethanol 1.0%,TNF -α+yellow wine 1.0%,TNF -α+ethanol 1.5%,and TNF -α+yellow wine 1.5% and the cells were given the corresponding treatment.NO production of culture supernatant was determined by nitrate reduction method and eNOS activity of cells was measured by chemical colorimetric method after the corresponding treatment for 24 h.The expression of eNOS and iNOS protein were detected by western blotting after the corresponding treatment for 48 h.Results Compared with the TNF -αgroup,NO production,eNOS activity,and eNOS protein expression in the rosuvastatin,and yellow wine 1.0%,and 1.5% groups were significantly increased and expression of iNOS protein were significantly decreased.Compared with the rosuvastatin group,eNOS protein expression in yellow wine 0.5% and yellow wine 0.5% groups significantly decreased.The expression of iNOS protein were significantly increased. Compared with ethanol groups,eNOS protein expression in yellow wine 0.5% and yellow wine 0.5% groups significantly increased and expression of iNOS protein were significantly decreased.Conclusion Treatment with yellow wine increased NO production,eNOS activity,and eNOS protein expression,which decreases iNOS protein expression.We conclude that yellow wine has similar beneficial effects as rosuvastatin on the cardiovascular system.These effects may be attributed to their anti -atherosclerotic actions.
