CAJ | 학술논문

本文利用串列加速器加速的21 MeV的质子束流对人正常淋巴细胞AHH‐1进行辐照，研究了质子诱导AHH‐1细胞PIG3基因的mRNA表达量和细胞周期改变的剂量和时间相关性。实时定量聚合酶链式反应（PCR）结果显示，质子辐照后，PIG3基因mRNA的表达量随剂量的增大而增大，表现出很好的剂量相关性。在不同的剂量点下，表达量随时间的变化趋势也大致相似，峰值出现的时间点除8 Gy剂量点出现在12 h外，其他剂量点均在照后6 h达到。理论拟合曲线和实验数据也呈现出很好的一致性。细胞周期检测结果显示，在每个剂量点下，G2／M期细胞随着时间的延长呈现先增加后减少的趋势。以上结果说明PIG3基因mRNA的表达量在生物剂量计的应用上可能具有一定的基础和潜力，值得进一步研究。
본문이용천렬가속기가속적21 MeV적질자속류대인정상림파세포AHH‐1진행복조，연구료질자유도AHH‐1세포PIG3기인적mRNA표체량화세포주기개변적제량화시간상관성。실시정량취합매련식반응（PCR）결과현시，질자복조후，PIG3기인mRNA적표체량수제량적증대이증대，표현출흔호적제량상관성。재불동적제량점하，표체량수시간적변화추세야대치상사，봉치출현적시간점제8 Gy제량점출현재12 h외，기타제량점균재조후6 h체도。이론의합곡선화실험수거야정현출흔호적일치성。세포주기검측결과현시，재매개제량점하，G2／M기세포수착시간적연장정현선증가후감소적추세。이상결과설명PIG3기인mRNA적표체량재생물제량계적응용상가능구유일정적기출화잠력，치득진일보연구。
AHH‐1 cell was irradiated with proton (21 MeV) at Q3D magnetic spectro‐meter of the tandem accelerator to study the dose‐and time‐effect of PIG3 gene mRNA expression in AHH‐1 cell .Real‐time quantitative PCR results show that PIG3 gene mRNA expression increases with doses at the different time after proton irradiation . And the PIG3 gene mRNA expression changes along with time at each dose .The peak of the PIG3 gene mRNA expression appears at 6 h for every dose except the 8 Gy at 12 h .T he results of cell cycle show that the percent of G 2/M first increases and then decreases with time . These results show that PIG3 gene mRNA expression in the application of biological dosimeter is potential .