CAJ | 학술논문

目的：了解新生儿接种不同类型乙型肝炎（乙肝）疫苗（HepB）后不同时期的免疫应答状况。方法选择余姚市和宁海县乙肝病毒表面抗原（HBsAg）阴性母亲2375人在2009年7月1日—12月31日分娩的新生儿作为研究对象，按照0、1、6个月免疫程序分别接种10μg 重组 HepB 和5μg 重组 HepB 疫苗，并在免后7个月、12个月和18个月分别采集静脉血进行免疫应答评价。结果免疫后7个月、12个月和18个月的抗－HBs 的几何平均浓度，5μg HepB －SCY 分别为（714．79±3．50）、（259．89±3．87）和（78．38±4．04）mIU ／mL，10μg HepB －HPY 分别为（2420．76±3．51）、（703．55±3．46）和（171．32±3．76）mIU ／mL，10μg 组的几何平均浓度均高于5μg 组（P 均＜0．01）。免后7、12、18月的抗－HBs 低／无应答（＜100 mIU ／mL）率，5μg 组分别为6．39％、23．33％和55．42％，10μg 组分别为2．17％、5．17％和31．95％，5μg 组均高于10μg 组（P 均＜0．01）。结论不同时期10μg 重组 HepB 的免疫应答均优于5μg 重组 HepB。
목적：료해신생인접충불동류형을형간염（을간）역묘（HepB）후불동시기적면역응답상황。방법선택여요시화저해현을간병독표면항원（HBsAg）음성모친2375인재2009년7월1일—12월31일분면적신생인작위연구대상，안조0、1、6개월면역정서분별접충10μg 중조 HepB 화5μg 중조 HepB 역묘，병재면후7개월、12개월화18개월분별채집정맥혈진행면역응답평개。결과면역후7개월、12개월화18개월적항－HBs 적궤하평균농도，5μg HepB －SCY 분별위（714．79±3．50）、（259．89±3．87）화（78．38±4．04）mIU ／mL，10μg HepB －HPY 분별위（2420．76±3．51）、（703．55±3．46）화（171．32±3．76）mIU ／mL，10μg 조적궤하평균농도균고우5μg 조（P 균＜0．01）。면후7、12、18월적항－HBs 저／무응답（＜100 mIU ／mL）솔，5μg 조분별위6．39％、23．33％화55．42％，10μg 조분별위2．17％、5．17％화31．95％，5μg 조균고우10μg 조（P 균＜0．01）。결론불동시기10μg 중조 HepB 적면역응답균우우5μg 중조 HepB。
Objective To know the immunologic response at different periods with different hepatitis B vaccine(HepB)in infants.Methods From July 1 to December 31 in 2009,the local infants born from hepatitis B virus(HBV)surface antigen (HBsAg)negative mother in Ninghai were selected and received a 0.5 ml of 5 -μg HepB made by recombinant deoxyribonucleic acid (DNA)techniques in saccharomyces cerevisiae yeast (HepB -SCY)and those in Yuyao were selected and received a 0.5 ml of 10 -μg HepB made by recombinant DNA techniques in hansenula polymorpha yeast (HepB -HPY)with a 0 -1 -6 month schedule.The specimens of blood were collected at 7 months,12 months and 18 months after vaccination for testing antibody.Results At 7 months,12 months and 18 months after vaccination,the geometric mean concentration (GMC)of Anti -HBs in HepB -SCY group were 714.79 ±3.50 mIU /mL,259.89 ± 3.87 mIU /mL and 78.38 ±4.04 mIU /mL,respectively.While in HepB -HPY group were 2 420.76 ±3.51 mIU /mL, 703.55 ±3.46 mIU /mL and 171.32 ±3.76 mIU /mL,respectively.There was statistical significant difference of GMC between HepB -SCY group and HepB -HPY group(P <0.01).The percent of antibody <100 mIU /mL in HepB -SCY group were 6.39%,23.33% and 55.42%,and in HepB -HPY group,were 2.17%,5.17% and 31.95%,respectively. The difference between HepB -SCY group and HepB -HPY group had significantly statistical significance (P <0.01 ). Conclusion The immunologic response at different periods of HepB -HPY was better than that of HepB -SCY.