CAJ | 학술논문

目的：探讨A型肉毒毒素（botulinum toxin A，BTXA）对增生性瘢痕组织成纤维细胞中TGF-β1和Ⅰ型胶原蛋白表达的影响。方法：组织块法培养增生性瘢痕组织及正常皮肤组织的成纤维细胞，用不同浓度的BTXA作用于体外培养的人增生性瘢痕成纤维细胞，检测MTT值；将浓度为0.2U/ml、0.4U/ml、0.8U/mlBTXA作用于增生性瘢痕成纤维细胞48h,利用RT-PCR检测BTXA对TGF-β1和Ⅰ型胶原蛋白mRNA的表达量。结果：BTXA在体外能明显抑制增生性瘢痕成纤维细胞的增殖，随着药物浓度的增加及时间的延长，其抑制作用明显增强，能够减少TGF-β1和Ⅰ型胶原蛋白mRNA的表达，且随着药物浓度的增加，TGF-β1和Ⅰ型胶原蛋白mRNA的表达量呈下降趋势。结论：BTXA通过抑制瘢痕组织中成纤维细胞的增殖，减少TGF-β1和Ⅰ型胶原蛋白mRNA的表达，减少细胞外基质的异常沉积来影响增生性瘢痕的形成。
목적：탐토A형육독독소（botulinum toxin A，BTXA）대증생성반흔조직성섬유세포중TGF-β1화Ⅰ형효원단백표체적영향。방법：조직괴법배양증생성반흔조직급정상피부조직적성섬유세포，용불동농도적BTXA작용우체외배양적인증생성반흔성섬유세포，검측MTT치；장농도위0.2U/ml、0.4U/ml、0.8U/mlBTXA작용우증생성반흔성섬유세포48h,이용RT-PCR검측BTXA대TGF-β1화Ⅰ형효원단백mRNA적표체량。결과：BTXA재체외능명현억제증생성반흔성섬유세포적증식，수착약물농도적증가급시간적연장，기억제작용명현증강，능구감소TGF-β1화Ⅰ형효원단백mRNA적표체，차수착약물농도적증가，TGF-β1화Ⅰ형효원단백mRNA적표체량정하강추세。결론：BTXA통과억제반흔조직중성섬유세포적증식，감소TGF-β1화Ⅰ형효원단백mRNA적표체，감소세포외기질적이상침적래영향증생성반흔적형성。
Objective To investigate the effect of botulinum toxin A (BTXA)on the expression of TGF-β1 and collagenⅠin hypertrophic scar fibroblast. Methods After treated with different concertrations of BTXA,the inhibition rate of hypertrophic scar fibroblasts were detected by MTT assay,0.2U/ml,0.4 U/ml and 0.8U/ml of BTXA was added in cultured hypertrophic scar fibroblast for 48h,the expression of TGF-β1 and collagenⅠin hypertrophic scar fibroblasts was respectively detected with RT-PCR. Results BTXA can significantly inhibit the proliferation of hypertrophic scar fibroblast,and the expression of TGF-β1and collagenⅠcould be inhibited significantly,the levels of the proliferation of hypertrophic scar fibro?blast and the expression of TGF-β1 and collagenⅠdecreased along with the increase in BTXA concer?tration. Conclusion BTXA inhibits hypertrophic scar formation through inhibiting the proliferation of hy?pertrophic scar fibroblast and down-regulating the expression of TGF-β1 and collagenⅠ.