国际医学寄生虫病杂志
國際醫學寄生蟲病雜誌
국제의학기생충병잡지
INTERNATIONAL JOURNAL OF MEDICAL PARASITIC DISEASES
2015年
2期
101-103
,共3页
许培培%刘峰%许晶晶%李浩%牛含青%何京%曹梓轩%冯龙%张耀元
許培培%劉峰%許晶晶%李浩%牛含青%何京%曹梓軒%馮龍%張耀元
허배배%류봉%허정정%리호%우함청%하경%조재헌%풍룡%장요원
羊肉%牛肉%弓形虫%套式PCR技术
羊肉%牛肉%弓形蟲%套式PCR技術
양육%우육%궁형충%투식PCR기술
Mutton%Beef%Toxoplasma gondii%Nested PCR
目的 了解新乡市2013年市售牛、羊肉弓形虫感染情况,为弓形虫病的防治提供依据.方法 随机采集新乡市40个农贸市场的牛、羊肌肉组织,其中市区牛肉71份、羊肉77份,郊区牛肉65份、羊肉64份,农村牛肉46份、羊肉45份,用套式PCR检测肉类弓形虫的感染情况. 结果 共检测牛肉182份,其中阳性23份,阳性率为12.64％ (23/182).市区、郊区、农村牛肉弓形虫阳性率依次为12.00％ (9/75)、12.50％ (8/64)和13.95％ (6/43).共检测羊肉191份,阳性22份,阳性率为11.52％ (22/191),市区、郊区、农村羊肉弓形虫阳性率依次为10.96％(8/73)、11.29％ (7/62)和12.50％ (7/56).牛、羊肉弓形虫阴性率在市区、郊区之间差异均无统计学意义(x2=2.153,x2=1.084,P＞0.05).市区、农村之间牛、羊肉阳性率差异有统计学意义(2=6.418,x2=7.005,P＜0.05). 结论 新乡市郊区及农村市售牛、羊肉有弓形虫感染.
目的 瞭解新鄉市2013年市售牛、羊肉弓形蟲感染情況,為弓形蟲病的防治提供依據.方法 隨機採集新鄉市40箇農貿市場的牛、羊肌肉組織,其中市區牛肉71份、羊肉77份,郊區牛肉65份、羊肉64份,農村牛肉46份、羊肉45份,用套式PCR檢測肉類弓形蟲的感染情況. 結果 共檢測牛肉182份,其中暘性23份,暘性率為12.64％ (23/182).市區、郊區、農村牛肉弓形蟲暘性率依次為12.00％ (9/75)、12.50％ (8/64)和13.95％ (6/43).共檢測羊肉191份,暘性22份,暘性率為11.52％ (22/191),市區、郊區、農村羊肉弓形蟲暘性率依次為10.96％(8/73)、11.29％ (7/62)和12.50％ (7/56).牛、羊肉弓形蟲陰性率在市區、郊區之間差異均無統計學意義(x2=2.153,x2=1.084,P＞0.05).市區、農村之間牛、羊肉暘性率差異有統計學意義(2=6.418,x2=7.005,P＜0.05). 結論 新鄉市郊區及農村市售牛、羊肉有弓形蟲感染.
목적 료해신향시2013년시수우、양육궁형충감염정황,위궁형충병적방치제공의거.방법 수궤채집신향시40개농무시장적우、양기육조직,기중시구우육71빈、양육77빈,교구우육65빈、양육64빈,농촌우육46빈、양육45빈,용투식PCR검측육류궁형충적감염정황. 결과 공검측우육182빈,기중양성23빈,양성솔위12.64％ (23/182).시구、교구、농촌우육궁형충양성솔의차위12.00％ (9/75)、12.50％ (8/64)화13.95％ (6/43).공검측양육191빈,양성22빈,양성솔위11.52％ (22/191),시구、교구、농촌양육궁형충양성솔의차위10.96％(8/73)、11.29％ (7/62)화12.50％ (7/56).우、양육궁형충음성솔재시구、교구지간차이균무통계학의의(x2=2.153,x2=1.084,P＞0.05).시구、농촌지간우、양육양성솔차이유통계학의의(2=6.418,x2=7.005,P＜0.05). 결론 신향시교구급농촌시수우、양육유궁형충감염.
Objective To investigate the infection of Toxoplasma gondii of commercial beef and mutton in Xinxiang City.Methods Beef and mutton samples were collected randomly at 40 farmer markets,of which,71 beef and 77 mutton samples from urban; 65 beef and 64 mutton samples from suburban; 46 beef and 45 muttonsamples from rural.The nested PCR was used to detect Toxoplasma gondii DNA in the muscle tissue.Results The total Toxoplasma positive rate was 12.64％(23/182) in beef samples.The Toxoplasma positive rates of beef samples from urban,suburban and rural were 12.00％(9/75),12.50％(8/64) and 13.95％(6/43),respectively.The total Toxoplasma positive rate of mutton samples was 11.52％(22/191).The Toxoplasma positive rates of mutton samples from urban,suburban and rural were 10.96％ (8/73),11.29％ (7/62)and 12.50％ (7/56),respectively.There was no statistically significant difference of beef and mutton positive rate between urban and suburban(x2-2.153,x2=1.084,P＞0.05).There was statistically significant difference of beef and mutton positive rate between urban and rural (x2=6.418,x2=7.005,P＜0.05).Conclusion The results showed that the commercial beef and mutton sold in Xinxiang City were infected with Toxoplasma gondii.
