国际医学寄生虫病杂志
國際醫學寄生蟲病雜誌
국제의학기생충병잡지
INTERNATIONAL JOURNAL OF MEDICAL PARASITIC DISEASES
2015年
2期
94-98
,共5页
王华素%徐金山%彭恒%李孟英%陈要朋%刘铁牛%朱淮民%薛绍礼
王華素%徐金山%彭恆%李孟英%陳要朋%劉鐵牛%硃淮民%薛紹禮
왕화소%서금산%팽항%리맹영%진요붕%류철우%주회민%설소례
血红扇头蜱%犬%巢式PCR%田鼠巴贝虫%立克次氏体
血紅扇頭蜱%犬%巢式PCR%田鼠巴貝蟲%立剋次氏體
혈홍선두비%견%소식PCR%전서파패충%립극차씨체
Rhipicephalus sanguineus%Dog%Nest-PCR%Babesia microti%Coxiella burnetii
目的 了解广西南宁某警犬养殖训练基地蟀虫及病原体携带情况. 方法 2013年7月在广西南宁某警犬养殖训练基地,采用逆毛式检蜱法采集犬体表以及犬舍墙壁的蜱虫,用巴贝虫属18S rRNA通用引物的巢式PCR、原核生物16S rRNA及真核生物线粒体16S rRNA通用引物的PCR和序列测定方法,鉴定蜱虫体内的病原体感染情况和蜱虫种类. 结果 从警犬体表及犬舍墙壁共计采集5只饱血蜱和13只饥饿蜱;经PCR鉴定均为血红扇头蜱(Rhipicephalus sanguineus).蜱虫体内扩增到田鼠巴贝虫(Babesia microti)、伯纳特氏立克次氏体(Goxiella burnetii)、假单胞球菌(Pseu-domonas sp.)、甲基杆菌(Methylobacterium sp.)DNA序列,阳性率分别为27.8％ (5/18)、22.2％ (4/18)、11.1％ (2/18)、11.1％ (2/18). 结论 南宁某警犬养殖训练基地蜱虫存在一定比例的田鼠巴贝虫、伯纳特氏立克次氏体、假单胞球菌、甲基杆菌感染阳性率,对接触人员、及其他牲畜有潜在感染的风险,应加强预防和控制.
目的 瞭解廣西南寧某警犬養殖訓練基地蟀蟲及病原體攜帶情況. 方法 2013年7月在廣西南寧某警犬養殖訓練基地,採用逆毛式檢蜱法採集犬體錶以及犬捨牆壁的蜱蟲,用巴貝蟲屬18S rRNA通用引物的巢式PCR、原覈生物16S rRNA及真覈生物線粒體16S rRNA通用引物的PCR和序列測定方法,鑒定蜱蟲體內的病原體感染情況和蜱蟲種類. 結果 從警犬體錶及犬捨牆壁共計採集5隻飽血蜱和13隻饑餓蜱;經PCR鑒定均為血紅扇頭蜱(Rhipicephalus sanguineus).蜱蟲體內擴增到田鼠巴貝蟲(Babesia microti)、伯納特氏立剋次氏體(Goxiella burnetii)、假單胞毬菌(Pseu-domonas sp.)、甲基桿菌(Methylobacterium sp.)DNA序列,暘性率分彆為27.8％ (5/18)、22.2％ (4/18)、11.1％ (2/18)、11.1％ (2/18). 結論 南寧某警犬養殖訓練基地蜱蟲存在一定比例的田鼠巴貝蟲、伯納特氏立剋次氏體、假單胞毬菌、甲基桿菌感染暘性率,對接觸人員、及其他牲畜有潛在感染的風險,應加彊預防和控製.
목적 료해엄서남저모경견양식훈련기지천충급병원체휴대정황. 방법 2013년7월재엄서남저모경견양식훈련기지,채용역모식검비법채집견체표이급견사장벽적비충,용파패충속18S rRNA통용인물적소식PCR、원핵생물16S rRNA급진핵생물선립체16S rRNA통용인물적PCR화서렬측정방법,감정비충체내적병원체감염정황화비충충류. 결과 종경견체표급견사장벽공계채집5지포혈비화13지기아비;경PCR감정균위혈홍선두비(Rhipicephalus sanguineus).비충체내확증도전서파패충(Babesia microti)、백납특씨립극차씨체(Goxiella burnetii)、가단포구균(Pseu-domonas sp.)、갑기간균(Methylobacterium sp.)DNA서렬,양성솔분별위27.8％ (5/18)、22.2％ (4/18)、11.1％ (2/18)、11.1％ (2/18). 결론 남저모경견양식훈련기지비충존재일정비례적전서파패충、백납특씨립극차씨체、가단포구균、갑기간균감염양성솔,대접촉인원、급기타생축유잠재감염적풍험,응가강예방화공제.
Objective To survey the infestation and pathogens infections of ticks at a police dog breed-ing and training base in Guangxi Nanning.Methods In July 2013,ticks from the police dogs and doghouses at a police dog breeding and training base were sampled; Using nest-PCR,PCR,with the universal primers of 18S rRNA of Babesia spp.,16S rRNA of prokaryote,and mitochondria 16S rRNA of eukaryote,the infection of pathogens in ticks was investigated.Results A total of 5 engorged ticks and 13 non-blood sucking ticks were collected.The ticks sampled were Rhipicephalus sanguincus identified by PCR.Some of the ticks from the police dogs and doghouses had positive amplifications of B.microti,C.burnetii,Pseudomonas sp.,Methylobacterium sp.,and the positive rates were 27.8％(5/18),22.2％(4/18),11.1％(2/18),11.1％(2/18),respectively.Conclusion Ticks at the police dog breeding and training base were found.The ticks were R.sanguineus according to the results of PCR amplification.And some of the tested ticks were infected with B.microti,C.burnetii,Pseudomonas sp.,Methylobacterium sp..Those microbes are zoonosic pathogens and are threatening to human and livestocks.Prevention and control strategy should be strengthened.
