CAJ | 학술논문

目的评估湖北省赤壁市农村儿童感染贾第虫情况,并初步进行类型分析,为加强贾第虫病的防治提供依据. 方法从赤壁市进行隐孢子虫调查的儿童粪便样本中,随机抽取20份样本,基于贾第虫磷酸丙糖异构酶(triosephosphate isomerase,tpi)基因,采用巢式PCR检测粪便标本中的贾第虫,对阳性样本的tpi基因片段进行测序,与GenBank中参考序列进行同源性比对分析,确定贾第虫的集聚体类型. 结果 PCR检测结果显示,1份样本为贾第虫阳性,测序后经分析鉴定为集聚体A,与美国威斯康辛州的污水厂污水分离株1503同源性为99％. 结论该地区农村儿童存在贾第虫感染,应加强贾第虫感染检测和卫生宣教.
목적 평고호북성적벽시농촌인동감염가제충정황,병초보진행류형분석,위가강가제충병적방치제공의거. 방법 종적벽시진행은포자충조사적인동분편양본중,수궤추취20빈양본,기우가제충린산병당이구매(triosephosphate isomerase,tpi)기인,채용소식PCR검측분편표본중적가제충,대양성양본적tpi기인편단진행측서,여GenBank중삼고서렬진행동원성비대분석,학정가제충적집취체류형. 결과 PCR검측결과현시,1빈양본위가제충양성,측서후경분석감정위집취체A,여미국위사강신주적오수엄오수분리주1503동원성위99％. 결론 해지구농촌인동존재가제충감염,응가강가제충감염검측화위생선교.
Objective To evaluate the Giardia infection in chidren from rural areas of Chibi City and analyze the subtype of Giardia based on the triosephosphate isomerase (tpi) gene so as to provide the evidence for the prevention and treatment of Giardia.Methods A total of 20 human fecal specimens were randomly selected from the samples of an investigation on Cryptosporidium from rural children in Chibi City.The DNA of stool samples was extracted and Giardia was detected through nested-PCR of Giarida tpi gene.Results The results of PCR detection identified one case as Giardia infection.By blasting sequence,it belonged to assemblage A,99％ homology with the 1503 isolate of wastewater samples in USA.Conclusion Giardia infection occurred in rural children in the investigated region where the detection of Giardia infection and health education should be strengthened.