CAJ | 학술논문

为研究温度和盐度对华贵栉孔扇贝免疫相关酶活力的联合效应，采用中心复合设计（CCD ）和响应曲面分析法（RSM ）进行试验，在实验室条件下研究了温度（19～31℃），盐度（22～38）对华贵栉孔扇贝酸性磷酸酶（ACP）、碱性磷酸酶（AKP）、谷胱甘肽还原酶（GR）三种免疫相关酶活性的联合效应，旨在考察温度与盐度对ACP ，AKP和GR活性的影响及华贵栉孔扇贝免疫水平较高时的最佳温度和盐度组合，为华贵栉孔扇贝人工养殖所需条件提供理论基础。结果表明：温度的一次效应和二次效应对ACP ，AKP和GR活力影响显著（P＜0．05）；盐度的一次效应和二次效应对ACP ，AKP和GR的活力影响显著（P＜0．05）；温度和盐度的交互作用对ACP ，AKP和GR活力的影响显著（P＜0．05）。采用响应曲面法，建立了温度和盐度对华贵栉孔扇贝ACP ，AKP和GR活性影响的模型方程，该模型方程决定系数分别为0．9866，0．9813和0．9576（P＜0．01），预测系数分别为0．9345，0．8880和0．7384，表明该模型可以用于预测华贵栉孔扇贝ACP ，AKP和GR活力变化。通过模型优化和验证实验，得到在温度24．30℃、盐度30．55时，ACP和AKP活力达到最小值，分别为170．43，181．74 U／mg ，而GR活力最大为0．34U／mg，满意度达0．95。
위연구온도화염도대화귀즐공선패면역상관매활력적연합효응，채용중심복합설계（CCD ）화향응곡면분석법（RSM ）진행시험，재실험실조건하연구료온도（19～31℃），염도（22～38）대화귀즐공선패산성린산매（ACP）、감성린산매（AKP）、곡광감태환원매（GR）삼충면역상관매활성적연합효응，지재고찰온도여염도대ACP ，AKP화GR활성적영향급화귀즐공선패면역수평교고시적최가온도화염도조합，위화귀즐공선패인공양식소수조건제공이론기출。결과표명：온도적일차효응화이차효응대ACP ，AKP화GR활력영향현저（P＜0．05）；염도적일차효응화이차효응대ACP ，AKP화GR적활력영향현저（P＜0．05）；온도화염도적교호작용대ACP ，AKP화GR활력적영향현저（P＜0．05）。채용향응곡면법，건립료온도화염도대화귀즐공선패ACP ，AKP화GR활성영향적모형방정，해모형방정결정계수분별위0．9866，0．9813화0．9576（P＜0．01），예측계수분별위0．9345，0．8880화0．7384，표명해모형가이용우예측화귀즐공선패ACP ，AKP화GR활력변화。통과모형우화화험증실험，득도재온도24．30℃、염도30．55시，ACP화AKP활력체도최소치，분별위170．43，181．74 U／mg ，이GR활력최대위0．34U／mg，만의도체0．95。
In order to provide a reference for disease control ,culture and spreading of Chlamys nobilis ,the synergistic effects of temperature (19~31 ℃) and salinity (22~38) on the activities of immune related en‐zymes of ACP ,AKP and GR were studied using the central composite design (CCD) and response surface methodology (RSM ) .The results showed that the linear effects of temperature on the activities of ACP , AKP and GR were significant (P<0 .05) .And quadratic effects of temperature on the activities of three enzymes were also significant (P<0 .05) ,which suggested that the activities of ACP ,AKP and GR were easily be impacted by the temperature conditions .While the linear effects of salinity on the activities of ACP ,AKP ,GR were significant (P<0 .05) ,and the quadratic effects of salinity on the ACP ,AKP and GR were also significant (P<0 .01) .There existed significant synergistic effects between temperature and salinity on the activities of ACP ,AKP and GR (P< 0 .05) .Through response surface methodology ,a model equation about the relationship of the activities of ACP ,AKP and GR to the two factors was estab‐lished ,with the R2 as high as 0 .986 6 ,0 .981 3 and 0 .957 6 (P<0 .05)respectively ,and the Pred .R2 reached to 0 .934 5 ,0 .888 0 and 0 .738 4 ,suggesting that the fitting capability of the model was practicably applied for prediction .Through the optimization of the reliable model ,the activities of ACP ,AKP reached their minimum(170 .43 ,181 .74 U/mg) and the activity of GR reached the maximum (0 .34 U/mg) when the 2‐factor combination was 24 .30 ℃ /30 .55 ,with the desirability value being 0 .95 .