CAJ | 학술논문

以金黄色葡萄球菌为指示菌，采用琼脂扩散法从国家海洋局海洋生物活性物质重点实验室广西钦州湾红树林微生物资源库中筛选获得一株具有明显抑菌效果的菌株5‐8，对该菌株进行了分子鉴定与系统发育分析。结果表明，此菌为欧文氏菌属（Erw inia）。通过单因子试验对其发酵条件进行了优化，优化后发酵条件为：最佳氮源为蛋白胨，最佳碳源为葡萄糖，发酵培养基初始pH值为8．0，发酵温度为25℃，盐度为1．5，摇瓶装液量30％，接种量为1％（V种子液∶V培养液＝1∶100）。
이금황색포도구균위지시균，채용경지확산법종국가해양국해양생물활성물질중점실험실엄서흠주만홍수림미생물자원고중사선획득일주구유명현억균효과적균주5‐8，대해균주진행료분자감정여계통발육분석。결과표명，차균위구문씨균속（Erw inia）。통과단인자시험대기발효조건진행료우화，우화후발효조건위：최가담원위단백동，최가탄원위포도당，발효배양기초시pH치위8．0，발효온도위25℃，염도위1．5，요병장액량30％，접충량위1％（V충자액∶V배양액＝1∶100）。
In this study ,an antimicrobial bacterium strain ,named 5‐8 ,was screened and isolated from man‐grove of Guang Xi by the method of agar diffusion using Staphylococcus aureu as the indicator strain .The results of molecular identification and phylogenetic analysis showed that this strain belonged to genera of Erwinia .The single factor experiments on the fermentation conditions were conducted and the optimized parameters were as follows :the best nitrogen source is peptone ,the best carbon source is glucose ,the starting pH of medium was 8 .0 ,fermentation temperature was 25℃ ,salinity was 1 .5% ,liquid volume in erlenmeyer flask was 30% and inoculation amount was 1% (Vseed liquid/Vnutrient solutio =1∶100) .