中国农业科学
中國農業科學
중국농업과학
SCIENTIA AGRICULTURA SINICA
2015年
8期
1579-1587
,共9页
黄瓜%黑籽南瓜%肉桂酸%根系边缘细胞%活性氧
黃瓜%黑籽南瓜%肉桂痠%根繫邊緣細胞%活性氧
황과%흑자남과%육계산%근계변연세포%활성양
cucumber%figleaf gourd%cinnamic acid%root border cells%reactive oxygen species
【目的】了解肉桂酸(CA)胁迫下根系边缘细胞(RBC)对黄瓜和黑籽南瓜活性氧代谢与根系活力的影响,探索RBC增强根尖抵御CA胁迫的生理机制,阐明黑籽南瓜抵御CA胁迫能力强于黄瓜的原因。【方法】选用‘中农16’黄瓜和云南黑籽南瓜种子,采用悬空培养的方法,待根系长到5 mm时分成两组。组1:每隔1 h对黄瓜和黑籽南瓜的初生根分别喷施0和0.25 mmol·L-1的CA水溶液(pH 6.0);组2:种子的初生根先用小水流(蒸馏水)每4 h冲洗一次,去除RBC,再用0和0.25 m mol·L-1的CA水溶液每1 h喷施根系1次。处理0、12、24和36 h时测量活性氧代谢指标;处理24 h时测量根系鲜重、根系呼吸速率和根系活力等指标。【结果】无CA胁迫时,有无RBC对根尖的鲜重和生理代谢没有显著影响。当根尖附着RBC时,CA胁迫导致黄瓜根系鲜重和根系活力下降,活性氧(ROS)水平和丙二醛(MDA)含量显著增加,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)及过氧化物酶(POD)的活性明显上升,呼吸速率和抗 CN 呼吸速率也明显上升;与黄瓜明显不同的是,CA 胁迫对黑籽南瓜根系鲜重、根系活力、ROS水平、MDA含量、SOD、CAT及POD的活性、呼吸速率和抗CN呼吸速率影响均不显著。RBC去除后, CA胁迫对黄瓜根系的生理代谢影响趋势和RBC去除之前相一致,胁迫程度更大;黑籽南瓜受到CA胁迫的影响与黄瓜基本一致,较RBC去除前差异显著。【结论】RBC通过降低根系中ROS水平及MDA含量,减轻了CA对黄瓜和黑籽南瓜根系的毒害;黑籽南瓜的 RBC 防御 CA 对自身根系毒害的能力明显强于黄瓜;若根尖没有 RBC 保护,CA对黄瓜和黑籽南瓜均造成明显的毒害。
【目的】瞭解肉桂痠(CA)脅迫下根繫邊緣細胞(RBC)對黃瓜和黑籽南瓜活性氧代謝與根繫活力的影響,探索RBC增彊根尖牴禦CA脅迫的生理機製,闡明黑籽南瓜牴禦CA脅迫能力彊于黃瓜的原因。【方法】選用‘中農16’黃瓜和雲南黑籽南瓜種子,採用懸空培養的方法,待根繫長到5 mm時分成兩組。組1:每隔1 h對黃瓜和黑籽南瓜的初生根分彆噴施0和0.25 mmol·L-1的CA水溶液(pH 6.0);組2:種子的初生根先用小水流(蒸餾水)每4 h遲洗一次,去除RBC,再用0和0.25 m mol·L-1的CA水溶液每1 h噴施根繫1次。處理0、12、24和36 h時測量活性氧代謝指標;處理24 h時測量根繫鮮重、根繫呼吸速率和根繫活力等指標。【結果】無CA脅迫時,有無RBC對根尖的鮮重和生理代謝沒有顯著影響。噹根尖附著RBC時,CA脅迫導緻黃瓜根繫鮮重和根繫活力下降,活性氧(ROS)水平和丙二醛(MDA)含量顯著增加,超氧化物歧化酶(SOD)、過氧化氫酶(CAT)及過氧化物酶(POD)的活性明顯上升,呼吸速率和抗 CN 呼吸速率也明顯上升;與黃瓜明顯不同的是,CA 脅迫對黑籽南瓜根繫鮮重、根繫活力、ROS水平、MDA含量、SOD、CAT及POD的活性、呼吸速率和抗CN呼吸速率影響均不顯著。RBC去除後, CA脅迫對黃瓜根繫的生理代謝影響趨勢和RBC去除之前相一緻,脅迫程度更大;黑籽南瓜受到CA脅迫的影響與黃瓜基本一緻,較RBC去除前差異顯著。【結論】RBC通過降低根繫中ROS水平及MDA含量,減輕瞭CA對黃瓜和黑籽南瓜根繫的毒害;黑籽南瓜的 RBC 防禦 CA 對自身根繫毒害的能力明顯彊于黃瓜;若根尖沒有 RBC 保護,CA對黃瓜和黑籽南瓜均造成明顯的毒害。
【목적】료해육계산(CA)협박하근계변연세포(RBC)대황과화흑자남과활성양대사여근계활력적영향,탐색RBC증강근첨저어CA협박적생리궤제,천명흑자남과저어CA협박능력강우황과적원인。【방법】선용‘중농16’황과화운남흑자남과충자,채용현공배양적방법,대근계장도5 mm시분성량조。조1:매격1 h대황과화흑자남과적초생근분별분시0화0.25 mmol·L-1적CA수용액(pH 6.0);조2:충자적초생근선용소수류(증류수)매4 h충세일차,거제RBC,재용0화0.25 m mol·L-1적CA수용액매1 h분시근계1차。처리0、12、24화36 h시측량활성양대사지표;처리24 h시측량근계선중、근계호흡속솔화근계활력등지표。【결과】무CA협박시,유무RBC대근첨적선중화생리대사몰유현저영향。당근첨부착RBC시,CA협박도치황과근계선중화근계활력하강,활성양(ROS)수평화병이철(MDA)함량현저증가,초양화물기화매(SOD)、과양화경매(CAT)급과양화물매(POD)적활성명현상승,호흡속솔화항 CN 호흡속솔야명현상승;여황과명현불동적시,CA 협박대흑자남과근계선중、근계활력、ROS수평、MDA함량、SOD、CAT급POD적활성、호흡속솔화항CN호흡속솔영향균불현저。RBC거제후, CA협박대황과근계적생리대사영향추세화RBC거제지전상일치,협박정도경대;흑자남과수도CA협박적영향여황과기본일치,교RBC거제전차이현저。【결론】RBC통과강저근계중ROS수평급MDA함량,감경료CA대황과화흑자남과근계적독해;흑자남과적 RBC 방어 CA 대자신근계독해적능력명현강우황과;약근첨몰유 RBC 보호,CA대황과화흑자남과균조성명현적독해。
[Objective]The aim of this paper was to clarify the defensive mechanism of root border cells (RBC) against cinnamic acid (CA) stress by analyzing the reactive oxygen metabolism and root activity of seedling RBC in cucumber (Cucumis sativus L.) and figleaf gourd (Cururbita ficifolia) under CA stress. [Method] The 5 mm length of aeroponicly cultured roots of cucumber cultivar Zhongnong No. 16 and figleaf gourd cultivar bouche were divided into two groups: one group was used to investigate reactive oxygen metabolism by spraying with 0 and 0.25 mmol·L-1 CA at 1 h interval, another group rinsing root tips of distilled water once every 4 h firstly and RBC were removed, then spraying 0 and 0.25 mmol·L-1 CA every 1 h. The metabolic index of active oxygen was measured after treatment for 0, 12, 24 and 36 hours of CA stress, root fresh weight, root respiration rate and root activity of the seedlings were measured after 24 hours. [Result]The root fresh weight and physiological metabolism were not affected significantly by RBC without CA stress. If the RBC were not removed, the root fresh weight and root activity decreased and the level of reactive oxygen species (ROS) and malonaldehyde (MDA), total respiration rate, CN-resistant respiration rate, the activities of superoxide dismutase (SOD), catalase (CAT) and guaiacol-dependent peroxidase (POD) increased significantly in cucumber under CA stress. However, the root fresh weight, root activity, the level of ROS and MDA, total respiration rate, CN-resistant respiration rate, the activities of SOD, CAT and POD were not affected significantly in figleaf gourd seedlings. If RBC were removed, the effect of CA stress on figleaf gourd was similar with cucumber, but significantly than RBC not removing. [Conclusion] RBC possibly attenuated the CA toxicity to the roots of cucumber and figleaf gourd through decreasing ROS and MDA content. The defensive ability of RBCs against CA on figleaf gourd was stronger than that of cucumber. Removal of RBCs could lead to great damage to the roots of cucumber and figleaf gourd significantly under CA stress.