CAJ | 학술논문

目的：研究姜黄素对心肌梗死大鼠的血流动力学以及基质金属蛋白酶-2( matrix metallo proteina-ses-2,MMP-2)表达的影响。方法将70只Sprague-Dawley成年雄性大鼠(250~300g),随机分为假手术组(n=15)和心肌梗死组(n=55),假手术组手术方式：左冠状动脉前降支处穿线并且打结,但是不阻断血流。心肌梗死组手术方式：结扎左冠状动脉前降支,阻断其冠状动脉的血流。24小时以后心肌梗死组进行随机分为：对照组、溶剂组和姜黄素组。姜黄素组腹腔注射姜黄素100mg/(kg·d),溶剂组腹腔注射相等容量的6%乙醇和6%聚乙二醇混合液。28天后用MP150型多通道生理信号采集处理系统测大鼠的左心室的舒张期末压、收缩压、最大变化速率、最小变化速率、平均动脉压。进行HE染色显微镜下观察大鼠心肌的形态学变化。采用免疫组化技术检测大鼠心肌MMP-2的表达情况。结果对照组和姜黄素组比较显示,姜黄素组收缩压、最大变化速率、最小变化速率明显提高,左心室的舒张期末压明显减低(P<0.05)。免疫组化结果显示,姜黄素可显著降低心肌MMP-2的表达(P<0.05)。 HE染色显示,梗死后正常心肌细胞梗死后消失,取而代之的是胶原蛋白。结论姜黄素可在一定程度上改善心肌梗死大鼠心脏功能,抑制MMP-2表达。
목적：연구강황소대심기경사대서적혈류동역학이급기질금속단백매-2( matrix metallo proteina-ses-2,MMP-2)표체적영향。방법장70지Sprague-Dawley성년웅성대서(250~300g),수궤분위가수술조(n=15)화심기경사조(n=55),가수술조수술방식：좌관상동맥전강지처천선병차타결,단시불조단혈류。심기경사조수술방식：결찰좌관상동맥전강지,조단기관상동맥적혈류。24소시이후심기경사조진행수궤분위：대조조、용제조화강황소조。강황소조복강주사강황소100mg/(kg·d),용제조복강주사상등용량적6%을순화6%취을이순혼합액。28천후용MP150형다통도생리신호채집처리계통측대서적좌심실적서장기말압、수축압、최대변화속솔、최소변화속솔、평균동맥압。진행HE염색현미경하관찰대서심기적형태학변화。채용면역조화기술검측대서심기MMP-2적표체정황。결과대조조화강황소조비교현시,강황소조수축압、최대변화속솔、최소변화속솔명현제고,좌심실적서장기말압명현감저(P<0.05)。면역조화결과현시,강황소가현저강저심기MMP-2적표체(P<0.05)。 HE염색현시,경사후정상심기세포경사후소실,취이대지적시효원단백。결론강황소가재일정정도상개선심기경사대서심장공능,억제MMP-2표체。
Objective The present study was designed to examine the effects of curcumin on hemodynamics and ma-trix metallo proteinases-2 ( MMP-2 ) expression in myocardial infarction rats. Method 70 cases Sprague-Dawley healthy adult male rats (250~300g) were randomly divided into sham operation group (n=15) and miocardial in-farction group ( MI, n=55 ) . The mode of operation was that the line in left anterior descending coronary artery threading and knotting but not to block the blood flow had used for sham operation group. MI group was established by ligation of left anterior descending branch of coronary artery and the sham operation group ( sham) rats underwent the same procedure without ligation. 24 hours later, the MI group was further sub-divided into 3 groups: control group, solvent group and curcumin group. The solvent group was treated with only solvent( a blend which blend Po-lyethylene glycol, alcohol and water)and curcumin group was intraperitoneally administered with curumin at 100mg/kg/d for 28 days. MP150 type multichannel physiological functions signal collection processing system was used to test left ventricular end diastolic pressure ( LVDEP) , systolic pressure ( SYS) , maximum change in pressure over the cycle (dp/dtmax), minimum change in pressure over the cycle (dp/dtmin) and mean blood pressure (MBP). HE stalning was employed to detect the morphological changes of cardiomyocytes. Moreover, the expression of MMP-2 protein in the myocardium of the rats was tested by immunohistochemical technique. Result SYS, dp/dtmax and dp/dtmin were significantly increased while the LVDEP decreased in the curcumin group compared with control (P<0. 05). Immunohistochemistry revealed a decrease in expression of MMP2 in curcumin group compared to con-tral and solvent groups(P<0. 05). HE stalning revealed that some normal cardiac myocytes were disappeared after MI, and were replaced by collagens. Conclusion Our study revealed that curcumin ameliorated hemodynamics and inhibit MMP-2 expression post myocardial infarction in rats.