CAJ | 학술논문

目的：研究姜黄素眼用脂质体的角膜透过性。方法：采用乙醇注入法制备姜黄素脂质体，以三甲基壳聚糖对脂质体囊泡进行包覆；采用过膜法、粒径及电位测定仪对脂质体进行理化性质分析；采用离体兔角膜透过实验及共聚焦激光扫描显微镜成像技术，对三甲基壳聚糖包覆的脂质体的角膜透过作用进行研究。结果：三甲基壳聚糖对脂质体的包覆对脂质体的包封率无明显影响，但粒径、电位有所增大，同时角膜透过显著高于未包覆脂质体组，随着作用时间的延长，可促进脂质体囊泡渗透至角膜上皮深层60μm处，并主要以细胞旁路途径发生角膜透过。结论：该研究表明三甲基壳聚糖对脂质体囊泡具有明显的角膜促透作用。
목적：연구강황소안용지질체적각막투과성。방법：채용을순주입법제비강황소지질체，이삼갑기각취당대지질체낭포진행포복；채용과막법、립경급전위측정의대지질체진행이화성질분석；채용리체토각막투과실험급공취초격광소묘현미경성상기술，대삼갑기각취당포복적지질체적각막투과작용진행연구。결과：삼갑기각취당대지질체적포복대지질체적포봉솔무명현영향，단립경、전위유소증대，동시각막투과현저고우미포복지질체조，수착작용시간적연장，가촉진지질체낭포삼투지각막상피심층60μm처，병주요이세포방로도경발생각막투과。결론：해연구표명삼갑기각취당대지질체낭포구유명현적각막촉투작용。
Objective:To study the effect of N-trimethyl chitosan as coating materials on corneal permeability of curcumin.Methods:Curcumin-loaded liposomes were prepared by ethanol injection method, and the vesicles were coated with trimethyl chitosan.Mem-brane filter, dynamic light scattering and electrophoretic light scattering were employed to study the physicochemical parameters.Cor-neal permeability of trimethyl chitosan-coated liposomes was carried out by transcorneal permeation study and confocal laser scanning microscopy ( CLSM) .Results:It was shown that the entrapment efficiency was not changed significantly with enlarged size and zeta po-tential with the coating of TMC.The TMC-coated liposomes produced the more pronounced corneal permeation, compared with the uncoated preparations.With the increased contact time, the fluorescence in rabbit corneal epithelium can be detected down to 60 μm below the surface via paracellular route.Conclusion:TMC has significant effect on the transport of curcumin through rabbit cornea in vitro.