湖北大学学报（自然科学版）
湖北大學學報（自然科學版）
호북대학학보（자연과학판）
2015年
3期
276-281
,共6页
盐酸川芎嗪%BSA%相互作用%金属离子
鹽痠川芎嗪%BSA%相互作用%金屬離子
염산천궁진%BSA%상호작용%금속리자
ligustrazine hydrochloride%bovine serum albumin%interaction%metal ions
在优化的实验条件下，利用光谱法研究盐酸川芎嗪（LH）和牛血清白蛋白（ BSA ）的相互作用和共存金属离子（Mg2+、Cu2+、Zn2+、Ni2+、Fe3+）对LH与BSA相互作用的影响.结果表明：LH对BSA的荧光有猝灭作用，其猝灭过程属于静态猝灭.通过计算得出LH与BSA的结合常数Kb及结合位点数n.根据热力学参数确定了LH与BSA之间的作用力类型主要为疏水作用力.结合位点位于BSA的亚结构ⅡA中，Hill系数nH＜1，表明LH有弱的负协同作用.同步荧光光谱显示两者的结合位点更接近于色氨酸，同时也研究了金属离子对二者作用的影响.为后续吡嗪类药物的研发和进一步探讨LH在生物体内与蛋白质的作用机制和生物学效应提供了理论依据.①
在優化的實驗條件下，利用光譜法研究鹽痠川芎嗪（LH）和牛血清白蛋白（ BSA ）的相互作用和共存金屬離子（Mg2+、Cu2+、Zn2+、Ni2+、Fe3+）對LH與BSA相互作用的影響.結果錶明：LH對BSA的熒光有猝滅作用，其猝滅過程屬于靜態猝滅.通過計算得齣LH與BSA的結閤常數Kb及結閤位點數n.根據熱力學參數確定瞭LH與BSA之間的作用力類型主要為疏水作用力.結閤位點位于BSA的亞結構ⅡA中，Hill繫數nH＜1，錶明LH有弱的負協同作用.同步熒光光譜顯示兩者的結閤位點更接近于色氨痠，同時也研究瞭金屬離子對二者作用的影響.為後續吡嗪類藥物的研髮和進一步探討LH在生物體內與蛋白質的作用機製和生物學效應提供瞭理論依據.①
재우화적실험조건하，이용광보법연구염산천궁진（LH）화우혈청백단백（ BSA ）적상호작용화공존금속리자（Mg2+、Cu2+、Zn2+、Ni2+、Fe3+）대LH여BSA상호작용적영향.결과표명：LH대BSA적형광유졸멸작용，기졸멸과정속우정태졸멸.통과계산득출LH여BSA적결합상수Kb급결합위점수n.근거열역학삼수학정료LH여BSA지간적작용력류형주요위소수작용력.결합위점위우BSA적아결구ⅡA중，Hill계수nH＜1，표명LH유약적부협동작용.동보형광광보현시량자적결합위점경접근우색안산，동시야연구료금속리자대이자작용적영향.위후속필진류약물적연발화진일보탐토LH재생물체내여단백질적작용궤제화생물학효응제공료이론의거.①
Under the optimal conditions,the interaction of ligustrazine hydrochloride(LH)with bovine serum albumin(BSA)was investigated by spectroscopic methods. The effects of metal ions(Mg2+、Cu2+、Zn2+、Ni2+、Fe3+)on the interaction LH with BSA were also discussed. The results showed that the fluorescence of BSA was quenched by LH,which was a static quenching process. The binding constants Kb and the corresponding number of binding sites n were obtained by calculation. The thermodynamic parameters obtained from measured data showed that the interaction of LH and BSA was mainly driven by hydrophobic force. The primary binding site for LH was located at siteⅠin sub-domainⅡA of BSA. The values of Hill’s coefficients were less than 1,which indicated that there was weakly negative cooperative effect. Synchronous fluorescence indicated that the binding site of LH and BSA was near by tryptophan residue. Moreover,the interaction between LH and BSA under metal ions was also studied. The theoretical basis was provided for further research on pyrazine drugs and the further study of the inner mechanism and biological effect in organism of LH and the proteins.
