中华创伤骨科杂志
中華創傷骨科雜誌
중화창상골과잡지
CHINESE JOURNAL OF ORTHOPAEDIC TRAUMA
2015年
4期
322-325
,共4页
骨和骨组织%运动%强度%大鼠
骨和骨組織%運動%彊度%大鼠
골화골조직%운동%강도%대서
Bone and bones%Exercise%Intensity%Rats
目的 探讨不同强度跑台运动对大鼠胫骨干骺端皮质骨和松质骨成分变化的影响.方法 将24只雌性SD大鼠随机分为4组(n=6):低强度组、中等强度组、高强度组和自由活动组,前3组SD大鼠按照不同运动强度跑步方案(分别为15 m/min、0°坡度,20 m/min、5°坡度,25 m/min、10°坡度)进行8周训练(每周5d,1 h/d),自由活动组作为对照组.8周后分别取各组大鼠胫骨左侧干骺端皮质骨和松质骨,应用拉曼光谱检测并分析各组的矿物质/基质比值、碳酸盐/磷酸盐比值及结晶度等. 结果 骨组织中矿物质/基质比值:中等强度组皮质骨平均为4.883±0.128,松质骨平均为4.216±0.213,均显著高于自由活动组(4.113±0.132、3.773±0.122);高强度组松质骨平均为3.222±0.329,显著低于自由活动组,差异均有统计学意义(P＜0.05).骨组织中碳酸盐/磷酸盐比值:中等强度组松质骨平均为0.166±0.013,显著低于自由活动组(0.177±0.011);高强度组皮质骨平均为0.195±0.012,松质骨平均为0.187±0.010,均显著高于自由活动组(0.183±0.014、0.177±0.011),差异均有统计学意义(P＜0.05).高强度组皮质骨和松质骨骨组织结晶度平均分别为0.052 1±0.001 2和0.052 2±0.001 7,,均显著高于其他3组,差异有统计学意义(P＜0.05). 结论 中等强度跑台运动能提高骨组织的矿化和转换,增强骨的质量;高强度运动会降低骨的矿化,降低骨的质量.
目的 探討不同彊度跑檯運動對大鼠脛骨榦骺耑皮質骨和鬆質骨成分變化的影響.方法 將24隻雌性SD大鼠隨機分為4組(n=6):低彊度組、中等彊度組、高彊度組和自由活動組,前3組SD大鼠按照不同運動彊度跑步方案(分彆為15 m/min、0°坡度,20 m/min、5°坡度,25 m/min、10°坡度)進行8週訓練(每週5d,1 h/d),自由活動組作為對照組.8週後分彆取各組大鼠脛骨左側榦骺耑皮質骨和鬆質骨,應用拉曼光譜檢測併分析各組的礦物質/基質比值、碳痠鹽/燐痠鹽比值及結晶度等. 結果 骨組織中礦物質/基質比值:中等彊度組皮質骨平均為4.883±0.128,鬆質骨平均為4.216±0.213,均顯著高于自由活動組(4.113±0.132、3.773±0.122);高彊度組鬆質骨平均為3.222±0.329,顯著低于自由活動組,差異均有統計學意義(P＜0.05).骨組織中碳痠鹽/燐痠鹽比值:中等彊度組鬆質骨平均為0.166±0.013,顯著低于自由活動組(0.177±0.011);高彊度組皮質骨平均為0.195±0.012,鬆質骨平均為0.187±0.010,均顯著高于自由活動組(0.183±0.014、0.177±0.011),差異均有統計學意義(P＜0.05).高彊度組皮質骨和鬆質骨骨組織結晶度平均分彆為0.052 1±0.001 2和0.052 2±0.001 7,,均顯著高于其他3組,差異有統計學意義(P＜0.05). 結論 中等彊度跑檯運動能提高骨組織的礦化和轉換,增彊骨的質量;高彊度運動會降低骨的礦化,降低骨的質量.
목적 탐토불동강도포태운동대대서경골간후단피질골화송질골성분변화적영향.방법 장24지자성SD대서수궤분위4조(n=6):저강도조、중등강도조、고강도조화자유활동조,전3조SD대서안조불동운동강도포보방안(분별위15 m/min、0°파도,20 m/min、5°파도,25 m/min、10°파도)진행8주훈련(매주5d,1 h/d),자유활동조작위대조조.8주후분별취각조대서경골좌측간후단피질골화송질골,응용랍만광보검측병분석각조적광물질/기질비치、탄산염/린산염비치급결정도등. 결과 골조직중광물질/기질비치:중등강도조피질골평균위4.883±0.128,송질골평균위4.216±0.213,균현저고우자유활동조(4.113±0.132、3.773±0.122);고강도조송질골평균위3.222±0.329,현저저우자유활동조,차이균유통계학의의(P＜0.05).골조직중탄산염/린산염비치:중등강도조송질골평균위0.166±0.013,현저저우자유활동조(0.177±0.011);고강도조피질골평균위0.195±0.012,송질골평균위0.187±0.010,균현저고우자유활동조(0.183±0.014、0.177±0.011),차이균유통계학의의(P＜0.05).고강도조피질골화송질골골조직결정도평균분별위0.052 1±0.001 2화0.052 2±0.001 7,,균현저고우기타3조,차이유통계학의의(P＜0.05). 결론 중등강도포태운동능제고골조직적광화화전환,증강골적질량;고강도운동회강저골적광화,강저골적질량.
Objective To evaluate the effects of different intensities of treadmill running on changes in tibial bone composition in a rat model.Methods A total of 24 female Wistar rats were randomly assigned into 4 even groups (n =6) of free movement (FM),low-intensity running (LR),medium-intensity running (MR),and high-intensity running (HR).Rats in LR,MR,and HR groups underwent treadmill running with respective intensities (15 m/min and a 0° gradient,20 m/min and a 5° gradient,25 m/min and a 10° gradient) for 8 weeks (1 h/d and 5 d/week).FM group were taken as controls.The cortical and trabecular bone at the left tibial metaphysis was harvested after 8 weeks in each group for Raman spectroscopy to analyze mineral-matrix ratio,carbonate-phosphate ratio and crystallinity.Results In MR group,the mean mineral-matrix ratio was 4.883 ± 0.128 for cortical bone and 4.216 ±0.213 for trabecular bone,both significantly higher than those in FM group (4.113 ±0.132 and 3.773 ±0.122) (P ＜ 0.05).The mean mineral-matrix ratio was 3.222 ± 0.329 for trabecular bone in HR group,significantly lower than that in FM group (P ＜ 0.05).In MR group,the mean carbonate-phosphate ratio was 0.166 ± 0.013 for trabecular bone,significantly lower than that in FM group (0.177 ± 0.011) (P ＜ 0.05).In HR group,the mean carbonate-phosphate ratio was 0.195 ± 0.012 for cortical bone and 0.187 ± 0.010 for trabecular bone,both significantly higher than those in FM group (0.183 ± 0.014 and 0.177 ± 0.011) (P ＜ 0.05).In HR group,the mean crystallinity was 0.0521 ± 0.0012 for cortical bone and 0.0522 ± 0.0017 for trabecular bone,both significantly higher than those in the other 3 groups (P ＜ 0.05).Conclusions Running with medium intensity may increase bone mineralization and turnover,thus enhancing bone quality.However,running with high intensity may decrease bone mineralization and thus bone quality.
