南方林业科学
南方林業科學
남방임업과학
Nanfang Forestry Science
2015年
2期
12-14,27
,共4页
罗坤水%胡庆%罗忠生%林洪%杨春霞
囉坤水%鬍慶%囉忠生%林洪%楊春霞
라곤수%호경%라충생%림홍%양춘하
沉水樟%组织培养
沉水樟%組織培養
침수장%조직배양
Cinnamomum micranthum%Tissue Culture
以沉水樟当年生半木质化枝条为材料,通过试验设计得到沉水樟最适诱导培养基为MS+6-BA 0.5 mg/L+NAA 0.2 mg/L(pH=6.0),最佳继代增殖培养基和生根培养基分别为1/2MS+6-BA 0.8 mg/L+NAA 0.05 mg/L+Na2S2O3150 mg/L(pH=5.5)和1/2MS+IBA 1.0 mg/L+NAA 0.05 mg/L,增殖系数达到2.5,有效生根率达到96%,且在增殖培养基中添加一定浓度的Na2S2O3,解决了新抽芽顶梢或叶枯顶或掉叶难题,为沉水樟快繁提供了一种有效方法。
以沉水樟噹年生半木質化枝條為材料,通過試驗設計得到沉水樟最適誘導培養基為MS+6-BA 0.5 mg/L+NAA 0.2 mg/L(pH=6.0),最佳繼代增殖培養基和生根培養基分彆為1/2MS+6-BA 0.8 mg/L+NAA 0.05 mg/L+Na2S2O3150 mg/L(pH=5.5)和1/2MS+IBA 1.0 mg/L+NAA 0.05 mg/L,增殖繫數達到2.5,有效生根率達到96%,且在增殖培養基中添加一定濃度的Na2S2O3,解決瞭新抽芽頂梢或葉枯頂或掉葉難題,為沉水樟快繁提供瞭一種有效方法。
이침수장당년생반목질화지조위재료,통과시험설계득도침수장최괄유도배양기위MS+6-BA 0.5 mg/L+NAA 0.2 mg/L(pH=6.0),최가계대증식배양기화생근배양기분별위1/2MS+6-BA 0.8 mg/L+NAA 0.05 mg/L+Na2S2O3150 mg/L(pH=5.5)화1/2MS+IBA 1.0 mg/L+NAA 0.05 mg/L,증식계수체도2.5,유효생근솔체도96%,차재증식배양기중첨가일정농도적Na2S2O3,해결료신추아정소혹협고정혹도협난제,위침수장쾌번제공료일충유효방법。
One-year half-lignified shoots from Cinnamomum micranthum trees were used to study the optimum media for tissue culture. The results showed that optimum induction medium for C. micranthum is MS+6-BA 0.5 mg/L+NAA 0.2 mg/L (pH=6.0). The best proliferation medium is 1/2MS+6-BA 0.8 mg/L+NAA 0.05 mg/L+ Na2S2O3 150 mg/L (pH=5.5), and its multiplication coefficient can reach 2.5. Rooting rate is above 96%in the optimum rooting medium that is 1/2MS+IBA1.0 mg/L +NAA0.05 mg/L. Moreover, proliferation medium containing certain concentration Na2S2O3 can efficiently resolve the difficult problem that the shoot top withered up or leaves fallen during the process of the proliferation culture, and provide an effective method for rapid propagation of C. micranthum.