CAJ | 학술논문

双向电泳(two-dimensional gel electrophoresis,2-DE)是蛋白质组学研究中常用的技术之一，因具有较高的稳定性和重复性，在植物蛋白质组学中应用广泛。蛋白质双向电泳(2-DE)过程复杂、时间持久以及影响因素众多，本文立足蛋白质双向电泳实验过程中可能出现的各种现象，如：分离的蛋白质点过少、条纹过多导致凝胶背景模糊、高丰度蛋白点聚集沉淀及低丰度蛋白点被掩盖等，着重分析其产生的原因并提出具体的解决办法，为蛋白质双向电泳技术初学者提供参考。
쌍향전영(two-dimensional gel electrophoresis,2-DE)시단백질조학연구중상용적기술지일，인구유교고적은정성화중복성，재식물단백질조학중응용엄범。단백질쌍향전영(2-DE)과정복잡、시간지구이급영향인소음다，본문립족단백질쌍향전영실험과정중가능출현적각충현상，여：분리적단백질점과소、조문과다도치응효배경모호、고봉도단백점취집침정급저봉도단백점피엄개등，착중분석기산생적원인병제출구체적해결판법，위단백질쌍향전영기술초학자제공삼고。
The different proteins have the different isoelectric point and molecular weight, and because of this, two-dimensional gel electrophoresis (2-DE) can separate the proteins mixture effectively. After some years development, the stability and repeatability of 2-DE have been improved greatly and now it has become one of the main core technologies in the proteomics research, as well as it has a widely application in plant proteomics. Some characteristics of 2-DE, such as a complex process, the long time and so on, can lead it failed in the end. In the process of 2-DE, there are often some phenomenon can affect the experiments' results containing so few proteins separated in the gel, much more stripes resulted in a very bad resolution, the high expression proteins often gathered and precipitated, and the low expression proteins point were be covered. The causes for the above phenomenon were analyzed and the resolve methods were put forward, and it provided references for beginners learning the technology of protein 2-DE.