中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2015年
4期
376-380
,共5页
孙树鹏%王琼%王修玉%王金环
孫樹鵬%王瓊%王脩玉%王金環
손수붕%왕경%왕수옥%왕금배
信号传导衔接蛋白%神经胶质瘤%细胞增殖%细胞凋亡
信號傳導銜接蛋白%神經膠質瘤%細胞增殖%細胞凋亡
신호전도함접단백%신경효질류%세포증식%세포조망
Signal transducing adaptor proteins%Glioma%Cell proliferation%Cell apoptosis
目的 探讨下调胶质瘤细胞株LN229中XB130的表达对细胞增殖和凋亡的影响.方法 选取LN229细胞作为研究对象,分为对照组、无义序列siRNA转染组和XB130 siRNA转染组进行细胞培养,细胞转染siRNA后,采用Western blot和免疫荧光检测XB130的表达情况;用MTT检测细胞的增殖活性;流式细胞仪检测细胞凋亡及周期.结果 转染siRNA后,对照组、无义序列siRNA转染组、XB130 siRNA转染组XB130蛋白相对表达量分别为1.00 ±0.10、1.00±0.13、0.52±0.06,XB130平均免疫荧光强度分别为0.085±0.012、0.072±0.009、0.013±0.002,表明XB130siRNA转染组XB130蛋白表达显著降低,差异有统计学意义(P＜0.05);MTr结果表明XB130 siRNA转染组细胞增殖受到明显抑制,差异有统计学意义(P＜0.05);流式细胞仪检测发现对照组、无义序列siRNA转染组、XB130 siRNA转染组细胞凋亡率分别为(12.45±0.96)％、(13.64±0.85)％、(20.26±1.42)％,G0/G1期细胞比例分别为(62.85±1.85)％、(63.04±1.74)％、(72.67±1.93)％,表明XB130 siRNA转染组细胞凋亡率显著高于其他两组,差异有统计学意义(P＜0.05),G0/G1期细胞比例也显著高于其他两组,差异有统计学意义(P＜0.05).结论 沉默胶质瘤细胞中XB130的表达能显著抑制细胞的增殖并促进其凋亡,表明XB130蛋白可促进胶质瘤细胞的增殖.
目的 探討下調膠質瘤細胞株LN229中XB130的錶達對細胞增殖和凋亡的影響.方法 選取LN229細胞作為研究對象,分為對照組、無義序列siRNA轉染組和XB130 siRNA轉染組進行細胞培養,細胞轉染siRNA後,採用Western blot和免疫熒光檢測XB130的錶達情況;用MTT檢測細胞的增殖活性;流式細胞儀檢測細胞凋亡及週期.結果 轉染siRNA後,對照組、無義序列siRNA轉染組、XB130 siRNA轉染組XB130蛋白相對錶達量分彆為1.00 ±0.10、1.00±0.13、0.52±0.06,XB130平均免疫熒光彊度分彆為0.085±0.012、0.072±0.009、0.013±0.002,錶明XB130siRNA轉染組XB130蛋白錶達顯著降低,差異有統計學意義(P＜0.05);MTr結果錶明XB130 siRNA轉染組細胞增殖受到明顯抑製,差異有統計學意義(P＜0.05);流式細胞儀檢測髮現對照組、無義序列siRNA轉染組、XB130 siRNA轉染組細胞凋亡率分彆為(12.45±0.96)％、(13.64±0.85)％、(20.26±1.42)％,G0/G1期細胞比例分彆為(62.85±1.85)％、(63.04±1.74)％、(72.67±1.93)％,錶明XB130 siRNA轉染組細胞凋亡率顯著高于其他兩組,差異有統計學意義(P＜0.05),G0/G1期細胞比例也顯著高于其他兩組,差異有統計學意義(P＜0.05).結論 沉默膠質瘤細胞中XB130的錶達能顯著抑製細胞的增殖併促進其凋亡,錶明XB130蛋白可促進膠質瘤細胞的增殖.
목적 탐토하조효질류세포주LN229중XB130적표체대세포증식화조망적영향.방법 선취LN229세포작위연구대상,분위대조조、무의서렬siRNA전염조화XB130 siRNA전염조진행세포배양,세포전염siRNA후,채용Western blot화면역형광검측XB130적표체정황;용MTT검측세포적증식활성;류식세포의검측세포조망급주기.결과 전염siRNA후,대조조、무의서렬siRNA전염조、XB130 siRNA전염조XB130단백상대표체량분별위1.00 ±0.10、1.00±0.13、0.52±0.06,XB130평균면역형광강도분별위0.085±0.012、0.072±0.009、0.013±0.002,표명XB130siRNA전염조XB130단백표체현저강저,차이유통계학의의(P＜0.05);MTr결과표명XB130 siRNA전염조세포증식수도명현억제,차이유통계학의의(P＜0.05);류식세포의검측발현대조조、무의서렬siRNA전염조、XB130 siRNA전염조세포조망솔분별위(12.45±0.96)％、(13.64±0.85)％、(20.26±1.42)％,G0/G1기세포비례분별위(62.85±1.85)％、(63.04±1.74)％、(72.67±1.93)％,표명XB130 siRNA전염조세포조망솔현저고우기타량조,차이유통계학의의(P＜0.05),G0/G1기세포비례야현저고우기타량조,차이유통계학의의(P＜0.05).결론 침묵효질류세포중XB130적표체능현저억제세포적증식병촉진기조망,표명XB130단백가촉진효질류세포적증식.
Objective To investigate the influence of XB130 on the proliferation and apoptosis of glioma cell LN229.Methods LN229 cells were divided into the control group,nonsense sequence siRNA transfected group and XB130 siRNA transfected group.After siRNAs were transfected into LN229 cells,the expression of XB130 was detected by Western blot and immunofluorescence staining.The phenotypic changes of LN229 cells including proliferation,apoptosis and cell cycle were determined by MTT assay and flow cytometry.Results The relative protein expression levels and immunofluorescence intensity of XB130 were respectively 1.00 ± 0.10 and 0.085 ± 0.012 in the control group,1.00 ± 0.13 and 0.072 ± 0.009 in the nonsense sequence group,0.52 ± 0.06 and 0.013 ± 0.002 in the XB130 siRNA transfected group.The expression of XB130 in LN229 cells was significantly decreased in the XB130 siRNA transfected group (P ＜ 0.05).LN229 cells transfected with XB130 siRNA showed lowering proliferation activity by MTT assay (P ＜ 0.05).The results of flow cytometry showed that the apoptotic rate and the proportion of cells in G0/G1 phase were (12.45 ± 0.96) ％ and (62.85 ± 1.85) ％ in the control group,(13.64 ± 0.85) ％ and (63.04 ± 1.74) ％ in the nonsense sequence group,(20.26 ± 1.42) ％ and (72.67 ± 1.93) ％ in the XB130 siRNA transfected group respectively.The apoptotic rate and the proportion of cells in G0/G1 phase in the XB130 siRNA transfected group were significantly higher than those in the other two groups (P ＜0.05).Conclusions Knocking down the expression of XB130 in human glioma cells could significantly inhibit cell proliferation and induce apoptosis,indicating XB130 could promote the proliferation of glioma cells.
