中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
4期
698-700
,共3页
黄沁园%何纯刚%陈利生%吴鸿根%邓洪强%潘云
黃沁園%何純剛%陳利生%吳鴻根%鄧洪彊%潘雲
황심완%하순강%진리생%오홍근%산홍강%반운
散发性结直肠癌%筛查诊断%粪便DNA甲基化%p33生长抑制基因1b
散髮性結直腸癌%篩查診斷%糞便DNA甲基化%p33生長抑製基因1b
산발성결직장암%사사진단%분편DNA갑기화%p33생장억제기인1b
Sporadic colorectal cancers%Screening diagnose%Fecal DNA methylation%p33 Inhibitor of Growth Gene 1b
目的 检测散发性结直肠癌患者癌组织及其配对的粪便DNA中p33生长抑制基因1b(p33ING1b)启动子甲基化,探讨其在两种标本中检测的一致性及其意义.方法 收集散发性结直肠癌组织及其配对的粪便标本及对照组粪便标本,采用巢式甲基化特异性聚合酶链反应(nMSP)检测p33ING1b基因启动子甲基化状况.结果 37例散发性结直肠癌组织、粪便中p33ING1b启动子甲基化阳性检出率分别为83.78％、78.38％,检测结果符合率为94.60％,具有明显相关性(r=0.838,P＜0.01).粪便DNA中p33ING1b启动子甲基化率明显高于对照组(P＜0.01),与癌组织临床病理特征无明显相关(P＞0.05).结论 检测粪便DNA中p33ING1b启动子甲基化能反映其在肿瘤组织中甲基化状况.
目的 檢測散髮性結直腸癌患者癌組織及其配對的糞便DNA中p33生長抑製基因1b(p33ING1b)啟動子甲基化,探討其在兩種標本中檢測的一緻性及其意義.方法 收集散髮性結直腸癌組織及其配對的糞便標本及對照組糞便標本,採用巢式甲基化特異性聚閤酶鏈反應(nMSP)檢測p33ING1b基因啟動子甲基化狀況.結果 37例散髮性結直腸癌組織、糞便中p33ING1b啟動子甲基化暘性檢齣率分彆為83.78％、78.38％,檢測結果符閤率為94.60％,具有明顯相關性(r=0.838,P＜0.01).糞便DNA中p33ING1b啟動子甲基化率明顯高于對照組(P＜0.01),與癌組織臨床病理特徵無明顯相關(P＞0.05).結論 檢測糞便DNA中p33ING1b啟動子甲基化能反映其在腫瘤組織中甲基化狀況.
목적 검측산발성결직장암환자암조직급기배대적분편DNA중p33생장억제기인1b(p33ING1b)계동자갑기화,탐토기재량충표본중검측적일치성급기의의.방법 수집산발성결직장암조직급기배대적분편표본급대조조분편표본,채용소식갑기화특이성취합매련반응(nMSP)검측p33ING1b기인계동자갑기화상황.결과 37례산발성결직장암조직、분편중p33ING1b계동자갑기화양성검출솔분별위83.78％、78.38％,검측결과부합솔위94.60％,구유명현상관성(r=0.838,P＜0.01).분편DNA중p33ING1b계동자갑기화솔명현고우대조조(P＜0.01),여암조직림상병리특정무명현상관(P＞0.05).결론 검측분편DNA중p33ING1b계동자갑기화능반영기재종류조직중갑기화상황.
Objective The aim of this study was to detect the promoter methylation status of p33 inhibitor of growth gene 1 b (p33ING1b) in tissue and the matched fecal DNA of sporadic colorectal cancers,and evaluate the consistency of the assays in the two samples and the clinical value.Methods Tissues with matched fecal samples and control group were collected from 37 patients of colorectal cancer.The promoter methylation status of p33ING1 b was detected by the nested methylation specific polymerase chain reaction (nMSP).Results Out of 37 cases,83.78％ and 78.38％ exhibited p33ING1b methylation in the tissue and matched fecal samples,respectively.The coincident rate of p33ING1 b methylation status in the two groups was 94.6％,with significant correlation (R =0.838,P ＜ 0.01).The methylated rate of p33ING1b in fecal samples of colorectal cancers (CRCs) was significantly higher than control group (P ＜ 0.01),and there was no significant relationship with clinical pathological factors of CRCs (P ＞ 0.05).Conclusion The p33ING1b methyaltion test in fecal DNA may reflect the real methylation status in CRCs and as a noninvasive test for screening CRCs.
