CAJ | 학술논문

目的观察微小RNA(miRNA,miR)-193b在结直肠癌(CRC)中的表达水平,探讨其作为CRC诊断标记的潜能.方法采用实时荧光定量聚合酶链反应(FQ-PCR)分析miR-193b在Ⅱ、Ⅲ、Ⅳ期及整体CRC中的表达水平,采用t检验及受试者特征曲线(ROC)分析miR-193b作为CRC诊断标记的敏感性和特异性.并应用Cluster聚类法分析其表达水平与CRC分期的相关性.结果 miR-193b在Ⅱ、Ⅲ、Ⅳ期及整体CRC中分别下调3.860倍(P＜0.05)、2.680倍(P＜0.05)、5.280倍(P＜0.05)、3.410倍(P＜0.05).miR-193b筛查CRC的特异性和敏感性分别是61.29％和77.42％,曲线下面积(AUC)为0.728.而其表达水平和CRC的分期无明显相关.结论 miR-193b具有作为CRC诊断标记的潜能.
목적 관찰미소RNA(miRNA,miR)-193b재결직장암(CRC)중적표체수평,탐토기작위CRC진단표기적잠능.방법 채용실시형광정량취합매련반응(FQ-PCR)분석miR-193b재Ⅱ、Ⅲ、Ⅳ기급정체CRC중적표체수평,채용t검험급수시자특정곡선(ROC)분석miR-193b작위CRC진단표기적민감성화특이성.병응용Cluster취류법분석기표체수평여CRC분기적상관성.결과 miR-193b재Ⅱ、Ⅲ、Ⅳ기급정체CRC중분별하조3.860배(P＜0.05)、2.680배(P＜0.05)、5.280배(P＜0.05)、3.410배(P＜0.05).miR-193b사사CRC적특이성화민감성분별시61.29％화77.42％,곡선하면적(AUC)위0.728.이기표체수평화CRC적분기무명현상관.결론 miR-193b구유작위CRC진단표기적잠능.
Objective To analysis the expression level of microRNA (miRNA,miR)-193b in colorectal cancer (CRC),and give a research on the sensitivity and specificity of distinguishing CRC from normal people.Methods Real time-Polymerase Chain Reaction was used to analysis the expression level of miR-193b between tumor and paired normal tissues in Ⅱ,Ⅲ,Ⅳ stages of CRC,t-test and Receiver operating characteristic (ROC) curve was performed to identify evaluate the specificity and sensitivity of using miR-193b as biomarker to discriminate CRC patients from normal person with P ＜0.05.Cluster was performed to find the relationship between the expressing level and cancer staging.Results miR-193b was performed down-regulated 3.860 folds (P ＜ 0.05),2.680 folds (P ＜ 0.05),5.280 folds (P ＜ 0.05)and 3.410 folds (P ＜0.05) in stage Ⅱ,Ⅲ,Ⅳ and mixtured cancer sample.MiR-193b could screening CRC from normal group with high sensitivity and specificity,but the dysregulation of miR-193b has no relation with pathological stage.Conclusion miR-193b showed high likely to be potential diagnostic marker of CRC.