CAJ | 학술논문

目的观察不同浓度七氟烷对幼年大鼠皮层脑电活动的影响.方法选择出生14 d大鼠,建立大脑皮层脑电图(EEG)监测模型,将大鼠随机分为3组:对照组(D组)、低浓度七氟烷组(S1组)、高浓度七氟烷组(S2组).动物模型建立后持续记录EEG.D组只记录EEG不给予七氟烷麻醉;S1组吸入6.0％七氟烷麻醉诱导,2.1％维持;S2组吸入8.0％的七氟烷诱导,3.5％维持,七氟烷麻醉1h停止吸入.记录麻醉诱导期间及维持期间EEG棘波的振幅和频率.实验结束后取血采用酶联免疫吸附试验法测定血清皮质酮浓度.结果麻醉诱导期间S2组棘波频率及振幅显著高于S1组(P＜0.05),麻醉维持期间S2组棘波频率高于D组(P＜0.05).D组、S1、S2组的皮质酮浓度分别为(26.8±3.8)、(106.5±15.1)、(155.9±13.6) μg/L,S1、S2组与D组比较差异有统计学意义(P＜0.05).结论七氟烷麻醉期间幼年大鼠皮层EEG可出现棘波,其机制可能与皮质酮有关.
목적 관찰불동농도칠불완대유년대서피층뇌전활동적영향.방법 선택출생14 d대서,건립대뇌피층뇌전도(EEG)감측모형,장대서수궤분위3조:대조조(D조)、저농도칠불완조(S1조)、고농도칠불완조(S2조).동물모형건립후지속기록EEG.D조지기록EEG불급여칠불완마취;S1조흡입6.0％칠불완마취유도,2.1％유지;S2조흡입8.0％적칠불완유도,3.5％유지,칠불완마취1h정지흡입.기록마취유도기간급유지기간EEG극파적진폭화빈솔.실험결속후취혈채용매련면역흡부시험법측정혈청피질동농도.결과 마취유도기간S2조극파빈솔급진폭현저고우S1조(P＜0.05),마취유지기간S2조극파빈솔고우D조(P＜0.05).D조、S1、S2조적피질동농도분별위(26.8±3.8)、(106.5±15.1)、(155.9±13.6) μg/L,S1、S2조여D조비교차이유통계학의의(P＜0.05).결론 칠불완마취기간유년대서피층EEG가출현극파,기궤제가능여피질동유관.
Objective To investigate the effects of sevoflurane on neonatal rats electrocephalography (EEG) and the mechanism.Methods Thirty neonatal rats,aged 14 days,weighing 35-45 g,were randomly divided into 3 groups:without sevoflurane group (group C,n =10),low sevoflurane concentration group (group S1,n =10),high sevoflurane concentration group (group S2).Electroencephalogram recording started immediately after completion of the implant surgery.Anesthesia was induced with 6.0％ sevoflurane over 3 min and maintained with 2.1％ over 60 min in group S1.In group S2 anesthesia was induced with 8.0％ sevoflurane and maintained with 3.5％.Acquisition of the EEG was performed using the Sirenia software.Sirenia Score programs were used for the EEG data analysis.Results As compared with group D and group S1,spike' s frequency and amplitude were significantly increased in group S2 during anesthesia induction.Spike' s frequency was higher in group S2 than group D during anesthesia maintenaed.The concentration of corticosterone among three groups were:26.8 ± 3.8,106.5 ± 15.1 and 155.9 ± 13.6,respectively.Compared with group D,the level of corticosterone were significantly increased in group S1 and group S2.Conclusion Sevoflurane anesthesia can induce spike EEG in 14-days rats,which may be induced by high level of corticosterone.