中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
4期
764-766
,共3页
余鹏飞%白槟%徐斌%郝一鸣%邱兆岩%王谦%冯全新%赵青川
餘鵬飛%白檳%徐斌%郝一鳴%邱兆巖%王謙%馮全新%趙青川
여붕비%백빈%서빈%학일명%구조암%왕겸%풍전신%조청천
急性胰腺炎%雨蛙素%模型,动物
急性胰腺炎%雨蛙素%模型,動物
급성이선염%우와소%모형,동물
Acute pancreatitis%Caerulein%Model,animal
目的 观察不同剂量雨蛙素小鼠腹腔注射时其胰腺炎严重程度的变化.方法 采用C57小鼠以雨蛙素50 μg/kg分别间断腹腔注射3、6、9、12次诱发胰腺炎,检测血清淀粉酶和脂肪酶、胰腺水含量、胰腺组织苏木素-伊红(HE)染色及组织学病理评分、肺脏组织HE染色.结果 常规雨蛙素剂量(50 μg/kg)分别注射3、6、9、12次时,实验组血清淀粉酶明显高于对照组(P＜0.05),在9次时达到高峰[(25 966.67±3 787.02) U/L];胰腺水含量在实验组中显著高于对照组(P＜0.05),注射6次时达到峰值[(81.13±1.03)％];胰腺组织HE染色结果显示注射3次胰腺HE染色仅有轻度水肿和少量炎细胞浸润,6次时可见大量中性粒细胞浸润,明显水肿,9次时胰腺小叶边缘出现少量的坏死灶,12次可见到大范围的腺泡坏死灶[组织病理评分(8.20±0.84)分],镜下表现出坏死性胰腺炎改变.结论 在C57小鼠体内,常规雨蛙素剂量(50 μg/kg)间断腹腔注射6次可诱发出急性水肿型胰腺炎,而12次可诱发出坏死性胰腺炎改变.
目的 觀察不同劑量雨蛙素小鼠腹腔註射時其胰腺炎嚴重程度的變化.方法 採用C57小鼠以雨蛙素50 μg/kg分彆間斷腹腔註射3、6、9、12次誘髮胰腺炎,檢測血清澱粉酶和脂肪酶、胰腺水含量、胰腺組織囌木素-伊紅(HE)染色及組織學病理評分、肺髒組織HE染色.結果 常規雨蛙素劑量(50 μg/kg)分彆註射3、6、9、12次時,實驗組血清澱粉酶明顯高于對照組(P＜0.05),在9次時達到高峰[(25 966.67±3 787.02) U/L];胰腺水含量在實驗組中顯著高于對照組(P＜0.05),註射6次時達到峰值[(81.13±1.03)％];胰腺組織HE染色結果顯示註射3次胰腺HE染色僅有輕度水腫和少量炎細胞浸潤,6次時可見大量中性粒細胞浸潤,明顯水腫,9次時胰腺小葉邊緣齣現少量的壞死竈,12次可見到大範圍的腺泡壞死竈[組織病理評分(8.20±0.84)分],鏡下錶現齣壞死性胰腺炎改變.結論 在C57小鼠體內,常規雨蛙素劑量(50 μg/kg)間斷腹腔註射6次可誘髮齣急性水腫型胰腺炎,而12次可誘髮齣壞死性胰腺炎改變.
목적 관찰불동제량우와소소서복강주사시기이선염엄중정도적변화.방법 채용C57소서이우와소50 μg/kg분별간단복강주사3、6、9、12차유발이선염,검측혈청정분매화지방매、이선수함량、이선조직소목소-이홍(HE)염색급조직학병리평분、폐장조직HE염색.결과 상규우와소제량(50 μg/kg)분별주사3、6、9、12차시,실험조혈청정분매명현고우대조조(P＜0.05),재9차시체도고봉[(25 966.67±3 787.02) U/L];이선수함량재실험조중현저고우대조조(P＜0.05),주사6차시체도봉치[(81.13±1.03)％];이선조직HE염색결과현시주사3차이선HE염색부유경도수종화소량염세포침윤,6차시가견대량중성립세포침윤,명현수종,9차시이선소협변연출현소량적배사조,12차가견도대범위적선포배사조[조직병리평분(8.20±0.84)분],경하표현출배사성이선염개변.결론 재C57소서체내,상규우와소제량(50 μg/kg)간단복강주사6차가유발출급성수종형이선염,이12차가유발출배사성이선염개변.
Objective To explore the severity of acute pancreatitis induced by intraperitoneal injection of 50 μg/kg caerulein at different doses in mice.Methods Acute pancreatitis (AP) was induced by intraperitoneal infection of 50 μg/kg caerulein at 3,6,9,12times,respectively.We evaluated the severity of AP biochemically and morphologically.Results Conventional doses of caerulein (50 μg/kg) were injected with 3,6,9,12 times,the level of serum amylase in experimental group were significantly higher than that in control group (P ＜ 0.05),peaked in the 9 times [(25 966.67 ± 3 787.02) U/L] ;pancreatic water content in the experimental group was significantly higher than that in control group (P ＜ 0.05),peaked in the 6 times [(81.13 ± 1.03) ％] ; pancreatic tissue hematoxylin and eosin (HE) staining showed that pancreas was slightly edema and infiltration of inflammatory cells at 3 times,which showed a large amount of neutrophil infiltration and obvious edema at the 6 times,and pancreatic leaflet margin appeared a small amount necrosis at the 9 times,and the acinar necrosis foci could be seen in large range an the 12 times (histopathological score 8.20 ±0.84),the microscope exhibited altered necrotizing pancreatitis.Conclnsion Conventional caerulein doses (50 μg/kg) may induce acute edematous pancreatitis by 6 times of intraperitoneal injection in mice,and 12 times may induce acute necrotizing pancreatitis.
