中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
4期
732-734
,共3页
多效生长因子%结直肠癌%原位杂交%免疫组织化学染色
多效生長因子%結直腸癌%原位雜交%免疫組織化學染色
다효생장인자%결직장암%원위잡교%면역조직화학염색
Pleiotropic growth factor%Colorectal cancer%In situ hybridization%Immunohistochemistry
目的 观察多效生长因子(PTN)在结直肠癌中表达.方法 选取我院接受治疗的83例结直肠癌患者设为观察组,选取同期到我院进行健康检查的志愿者54例设为对照组.采用原位杂交和免疫组织化学染色法检测结直肠癌组织和正常结直肠组织中PTN mRNA及其蛋白的表达,并分析其余结直肠癌患者临床病理特征的关系.结果 观察组患者的PTN mRNA及其蛋白的表达阳性率分别为63.9％和60.2％,对照组PTN mRNA及其蛋白的表达阳性率分别为40.7％和33.3％,观察组显著高于对照组,两组间差异有统计学意义(P＜0.05);观察组患者中PTN mRNA及蛋白的表达与年龄、性别、肿瘤类型均无明显相关(P＞0.05),而与分化程度、TNM分期明显相关(P＜0.05).而Ⅲ～Ⅳ期患者的PTN mRNA及蛋白的表达阳性率均高于Ⅰ～Ⅱ期患者,而高分化者均低于低分化者(P＜0.05).结论 PTN mRNA及其蛋白在结直肠癌组织中高表达,其可能参与了结直肠癌的浸润与转移.
目的 觀察多效生長因子(PTN)在結直腸癌中錶達.方法 選取我院接受治療的83例結直腸癌患者設為觀察組,選取同期到我院進行健康檢查的誌願者54例設為對照組.採用原位雜交和免疫組織化學染色法檢測結直腸癌組織和正常結直腸組織中PTN mRNA及其蛋白的錶達,併分析其餘結直腸癌患者臨床病理特徵的關繫.結果 觀察組患者的PTN mRNA及其蛋白的錶達暘性率分彆為63.9％和60.2％,對照組PTN mRNA及其蛋白的錶達暘性率分彆為40.7％和33.3％,觀察組顯著高于對照組,兩組間差異有統計學意義(P＜0.05);觀察組患者中PTN mRNA及蛋白的錶達與年齡、性彆、腫瘤類型均無明顯相關(P＞0.05),而與分化程度、TNM分期明顯相關(P＜0.05).而Ⅲ～Ⅳ期患者的PTN mRNA及蛋白的錶達暘性率均高于Ⅰ～Ⅱ期患者,而高分化者均低于低分化者(P＜0.05).結論 PTN mRNA及其蛋白在結直腸癌組織中高錶達,其可能參與瞭結直腸癌的浸潤與轉移.
목적 관찰다효생장인자(PTN)재결직장암중표체.방법 선취아원접수치료적83례결직장암환자설위관찰조,선취동기도아원진행건강검사적지원자54례설위대조조.채용원위잡교화면역조직화학염색법검측결직장암조직화정상결직장조직중PTN mRNA급기단백적표체,병분석기여결직장암환자림상병리특정적관계.결과 관찰조환자적PTN mRNA급기단백적표체양성솔분별위63.9％화60.2％,대조조PTN mRNA급기단백적표체양성솔분별위40.7％화33.3％,관찰조현저고우대조조,량조간차이유통계학의의(P＜0.05);관찰조환자중PTN mRNA급단백적표체여년령、성별、종류류형균무명현상관(P＞0.05),이여분화정도、TNM분기명현상관(P＜0.05).이Ⅲ～Ⅳ기환자적PTN mRNA급단백적표체양성솔균고우Ⅰ～Ⅱ기환자,이고분화자균저우저분화자(P＜0.05).결론 PTN mRNA급기단백재결직장암조직중고표체,기가능삼여료결직장암적침윤여전이.
Objective To investigate the pleiotropic growth factor (PTN) expression in colorectal cancer.Methods The hospital treated 83 cases of colorectal cancer patients to observation group,choose the same period to our hospital for a health check to volunteer 54 cases in the control group.The expression in situ hybridization and immunohistochemical staining to detect colorectal cancer tissues and normal rectal tissue PTN mRNA and protein,and analyze the relationship between clinical and pathological features of the rest of colorectal cancer.Results The expression rate of patients in the observation group and protein PTN mRNA 63.9％ and 60.2％,respectively,PTN mRNA and protein expression rate of the control group of volunteers was 40.7％ and 33.3％,respectively,the PTN mRNA and proten expression rate of patients in the observation group was significantly higher than the control group of volunteers,the difference was statistically significant (P ＜ 0.05) ; and the degree of differentiation observed in patients PTN mRNA and protein expression,TNM stage was significantly correlated (P ＜ 0.05).And Ⅲ-Ⅳ stage patients PTN mRNA and protein expression were significantly higher than Ⅰ-Ⅱ patients,while well-caught less than poorly differentiated (P ＜ 0.05).Conclusion PTN mRNA its protein is highly expressed in colorectal cancer tissues,which may be involved in invasion and metastasis of colorectal cancer.
