CAJ | 학술논문

目的检测大肠癌组织中Girdin蛋白的表达,探讨与大肠癌临床病理特征及侵袭转移的关系.方法应用免疫组织化学Envision二步法,对84例大肠癌及40例腺瘤、30例癌旁黏膜组织进行Girdin蛋白检测,统计分析其与临床病理参数之间的关系.结果 84例大肠癌组织中Girdin蛋白阳性表达率为42.9％,癌旁黏膜组织、低级别上皮内瘤变(LGIN)腺瘤及高级别上皮内瘤变(HGIN)腺瘤Girdin蛋白阳性表达率分别为3.3％、11.1％、18.2％,差异有统计学意义(x2=15.752,P＜0.01;x2=6.391,P＜0.05x2 =4.518,P＜0.05).大肠癌组织中Girdin蛋白的表达与肿瘤浸润深度、淋巴结转移及临床分期明显相关(P＜0.01),与性别、年龄、肿瘤大小、肿瘤部位、组织学分级无明显相关(P＞0.05).大肠癌Girdin蛋白阳性表达组的5年生存率为30.6％,阴性组5年生存率为83.3％,两组间差异有统计学意义(P＜0.01).结论 Girdin蛋白在大肠癌组织中呈高表达,与大肠癌的侵袭转移有关.
목적 검측대장암조직중Girdin단백적표체,탐토여대장암림상병리특정급침습전이적관계.방법 응용면역조직화학Envision이보법,대84례대장암급40례선류、30례암방점막조직진행Girdin단백검측,통계분석기여림상병리삼수지간적관계.결과 84례대장암조직중Girdin단백양성표체솔위42.9％,암방점막조직、저급별상피내류변(LGIN)선류급고급별상피내류변(HGIN)선류Girdin단백양성표체솔분별위3.3％、11.1％、18.2％,차이유통계학의의(x2=15.752,P＜0.01;x2=6.391,P＜0.05x2 =4.518,P＜0.05).대장암조직중Girdin단백적표체여종류침윤심도、림파결전이급림상분기명현상관(P＜0.01),여성별、년령、종류대소、종류부위、조직학분급무명현상관(P＞0.05).대장암Girdin단백양성표체조적5년생존솔위30.6％,음성조5년생존솔위83.3％,량조간차이유통계학의의(P＜0.01).결론 Girdin단백재대장암조직중정고표체,여대장암적침습전이유관.
Objective To investigate the expression of Girdin protein in colorectal carcinoma,and explore its relationship with clinical characteristics and tumor' s invasion and metastasis.Methods Eighty-four cases of colorectal carcinoma specimens,40 cases of colorectal adenoma and 30 cases of tumor-adjacent mucosal tissues were collected,and the expression levels of Girdin protein were detected by immumihistochemistry Envision two-step methods.The relationship between Girdin expression and clinicopathological characteristics was analyzed.Results The positive expression rate of Girdin protein in colorectal carcinoma was 42.9％,which was significantly higher than that in tumor-adjacent mucosa,adenoma with low grade inraepithelial neoplasia (LGIN) and adenoma with high grade inraepithelial neoplasia (HGIN) respectively (x2 =15.752,P ＜ 0.01 ;x2 =6.391,P ＜ 0.05 ;x2 =4.518,P ＜ 0.05).Overexpression of Girdin protein was correlated with invasion depth,lymph node metastasis and Dukes clinical stage (P ＜ 0.01),but not with gender,age,pathologic grade,tumor's size and localization (P ＞ 0.05).Follow-up study results showed that the five-year overall survival rate in Girdin negative group (83.3％) was higher than that in Twist positive group (21.7％) significantly (P ＜ 0.01).Conclusion Girdin protein expression is up-regulated in colorectal carcinoma and correlated with tumor's invasion and metastasis.