中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
4期
718-720
,共3页
张旸%曾育杰%蓝球生%许鹤洋%来伟%褚忠华
張旸%曾育傑%藍毬生%許鶴洋%來偉%褚忠華
장양%증육걸%람구생%허학양%래위%저충화
蛋白酪氨酸磷酸酶-3%LoVo%肝细胞%Snail
蛋白酪氨痠燐痠酶-3%LoVo%肝細胞%Snail
단백락안산린산매-3%LoVo%간세포%Snail
Phosphatase of regenerating liver-3%LoVo%Hepatic cell%Snail
目的 观察结肠癌肝转移的肿瘤微环境中肿瘤细胞与肝细胞(L02)相互作用及对肿瘤细胞Snail基因表达的影响.方法 将转入蛋白酪氨酸磷酸酶-3(PRL-3)的LoVo细胞和人正常肝细胞(L02)模拟肿瘤微环境进行共培养0～5d后,通过反转录-聚合酶链反应(RT-PCR)和Western blot检测LoVo细胞Snail蛋白表达,通过细胞划痕实验检测LoVo细胞迁移能力的改变.结果 用RT-PCR和Western blot检测发现PRL-3能通过共培养降低Snail基因的表达,蛋白灰度分析显示其抑制率分别为34.06％、37.82％、39.91％、64.07％、73.58％ (P＜0.01),而未转染的LoVo细胞Snail表达降低不明显,并且经过共培养后高表达PRL-3的LoVo细胞迁移能力明显降低(P＜0.05).结论 PRL-3在与肝细胞的共培养环境中下调肿瘤细胞Snail表达从而降低LoVo细胞的迁移能力.
目的 觀察結腸癌肝轉移的腫瘤微環境中腫瘤細胞與肝細胞(L02)相互作用及對腫瘤細胞Snail基因錶達的影響.方法 將轉入蛋白酪氨痠燐痠酶-3(PRL-3)的LoVo細胞和人正常肝細胞(L02)模擬腫瘤微環境進行共培養0～5d後,通過反轉錄-聚閤酶鏈反應(RT-PCR)和Western blot檢測LoVo細胞Snail蛋白錶達,通過細胞劃痕實驗檢測LoVo細胞遷移能力的改變.結果 用RT-PCR和Western blot檢測髮現PRL-3能通過共培養降低Snail基因的錶達,蛋白灰度分析顯示其抑製率分彆為34.06％、37.82％、39.91％、64.07％、73.58％ (P＜0.01),而未轉染的LoVo細胞Snail錶達降低不明顯,併且經過共培養後高錶達PRL-3的LoVo細胞遷移能力明顯降低(P＜0.05).結論 PRL-3在與肝細胞的共培養環境中下調腫瘤細胞Snail錶達從而降低LoVo細胞的遷移能力.
목적 관찰결장암간전이적종류미배경중종류세포여간세포(L02)상호작용급대종류세포Snail기인표체적영향.방법 장전입단백락안산린산매-3(PRL-3)적LoVo세포화인정상간세포(L02)모의종류미배경진행공배양0～5d후,통과반전록-취합매련반응(RT-PCR)화Western blot검측LoVo세포Snail단백표체,통과세포화흔실험검측LoVo세포천이능력적개변.결과 용RT-PCR화Western blot검측발현PRL-3능통과공배양강저Snail기인적표체,단백회도분석현시기억제솔분별위34.06％、37.82％、39.91％、64.07％、73.58％ (P＜0.01),이미전염적LoVo세포Snail표체강저불명현,병차경과공배양후고표체PRL-3적LoVo세포천이능력명현강저(P＜0.05).결론 PRL-3재여간세포적공배양배경중하조종류세포Snail표체종이강저LoVo세포적천이능력.
Objective To investigate the interaction between colon cancer cells and hepatic cell (L02),and it' s influence to Snail expression in tumor cells.Methods LoVo cells which transfected with phosphatase of regenerating liver-3 (PRL-3) and human hepatic cells (L02) simulated tumor microenvironment were cocultured by 0-5 day,reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting were used to examine Snail expression of LoVo cells,and the migration of LoVo cells also be detected.Results Used by RT-PCR and Western blotting it was found out that LoVo cells (transfected with PRL-3) were reduced Snail express after cocultured with hepatic cells,the inhibition rates were 34.06％,37.82％,39.91％,64.07％,73.58％ (P ＜ 0.05),LoVo cells (without transfected PRL-3) decreased expression of Snail was not obvious.Besides,coculture also depress the migration of LoVo cells.Conclusion PRL-3 depress the migration of LoVo cells through reduced Snail expression in liver metastasis of colon cancer microenvironment.
