中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
4期
687-690
,共4页
姜黎%周瑞%周峰%叶梅%汤小燕%夏上
薑黎%週瑞%週峰%葉梅%湯小燕%夏上
강려%주서%주봉%협매%탕소연%하상
多药耐药基因%单核苷酸多态性%大肠癌
多藥耐藥基因%單覈苷痠多態性%大腸癌
다약내약기인%단핵감산다태성%대장암
Multidrug resistance gene1%Single nucleotide polymorphism%Colorectal carcinoma
目的 探讨湖北汉族人群多药耐药基因(MDR1)3个位点的单核苷酸多态(1b外显子-129T→C突变、21外显子2 677G→T/A突变、26外显子3 435C→T突变)与大肠癌的相关性.方法 通过多重单碱基延伸反应[SNaPshot单核苷酸多态性(SNP)分型技术]检测133例大肠癌患者和383例对照组的MDR1 T-129C、G2 677T/A、C3 435T基因型分布.统计各位点基因型频率以及T-129C-G2 677T/A-C3 435T单倍体型频率.结合临床分型,比较不同基因型、单倍体型与大肠癌发病风险的相关性.结果 MDR1 T-129C位点的基因型分布频率在病例组与对照组差异有统计学意义.病例组中,MDR1 T-129C位点的C等位基因频率(6.4%)明显高于对照组中C等位基因频率(3.4%),差异有统计学意义(P<0.05).MDR1-129位点的基因型TC频率在大肠癌组(12.8%)较对照组(6.3%)显著增高,与大肠癌发病相关[比值比(OR)=2.186,P<0.05].MDR1的3个位点间-129、2677、3 435存在连锁不平衡性,共检出11种单倍体型(T-129C-G2 677T/A-C3 435T),其中以TGC (40.27%)、TTT(36.96%)、TAC (9.97%)、TTC (5.02%)4种单倍体型为主.单倍体型CGC、CTT在病例组(1.98%,1.25%)与对照组(0.51%,0.17%)中分布差异有统计学意义(P<0.05).单倍体型TGC/TTT在结肠癌组(35.5%)分布频率较直肠癌(15.5%)高(P<0.05).结论 MDR1 T-129C的基因多态性与大肠癌易感性明显相关.
目的 探討湖北漢族人群多藥耐藥基因(MDR1)3箇位點的單覈苷痠多態(1b外顯子-129T→C突變、21外顯子2 677G→T/A突變、26外顯子3 435C→T突變)與大腸癌的相關性.方法 通過多重單堿基延伸反應[SNaPshot單覈苷痠多態性(SNP)分型技術]檢測133例大腸癌患者和383例對照組的MDR1 T-129C、G2 677T/A、C3 435T基因型分佈.統計各位點基因型頻率以及T-129C-G2 677T/A-C3 435T單倍體型頻率.結閤臨床分型,比較不同基因型、單倍體型與大腸癌髮病風險的相關性.結果 MDR1 T-129C位點的基因型分佈頻率在病例組與對照組差異有統計學意義.病例組中,MDR1 T-129C位點的C等位基因頻率(6.4%)明顯高于對照組中C等位基因頻率(3.4%),差異有統計學意義(P<0.05).MDR1-129位點的基因型TC頻率在大腸癌組(12.8%)較對照組(6.3%)顯著增高,與大腸癌髮病相關[比值比(OR)=2.186,P<0.05].MDR1的3箇位點間-129、2677、3 435存在連鎖不平衡性,共檢齣11種單倍體型(T-129C-G2 677T/A-C3 435T),其中以TGC (40.27%)、TTT(36.96%)、TAC (9.97%)、TTC (5.02%)4種單倍體型為主.單倍體型CGC、CTT在病例組(1.98%,1.25%)與對照組(0.51%,0.17%)中分佈差異有統計學意義(P<0.05).單倍體型TGC/TTT在結腸癌組(35.5%)分佈頻率較直腸癌(15.5%)高(P<0.05).結論 MDR1 T-129C的基因多態性與大腸癌易感性明顯相關.
목적 탐토호북한족인군다약내약기인(MDR1)3개위점적단핵감산다태(1b외현자-129T→C돌변、21외현자2 677G→T/A돌변、26외현자3 435C→T돌변)여대장암적상관성.방법 통과다중단감기연신반응[SNaPshot단핵감산다태성(SNP)분형기술]검측133례대장암환자화383례대조조적MDR1 T-129C、G2 677T/A、C3 435T기인형분포.통계각위점기인형빈솔이급T-129C-G2 677T/A-C3 435T단배체형빈솔.결합림상분형,비교불동기인형、단배체형여대장암발병풍험적상관성.결과 MDR1 T-129C위점적기인형분포빈솔재병례조여대조조차이유통계학의의.병례조중,MDR1 T-129C위점적C등위기인빈솔(6.4%)명현고우대조조중C등위기인빈솔(3.4%),차이유통계학의의(P<0.05).MDR1-129위점적기인형TC빈솔재대장암조(12.8%)교대조조(6.3%)현저증고,여대장암발병상관[비치비(OR)=2.186,P<0.05].MDR1적3개위점간-129、2677、3 435존재련쇄불평형성,공검출11충단배체형(T-129C-G2 677T/A-C3 435T),기중이TGC (40.27%)、TTT(36.96%)、TAC (9.97%)、TTC (5.02%)4충단배체형위주.단배체형CGC、CTT재병례조(1.98%,1.25%)여대조조(0.51%,0.17%)중분포차이유통계학의의(P<0.05).단배체형TGC/TTT재결장암조(35.5%)분포빈솔교직장암(15.5%)고(P<0.05).결론 MDR1 T-129C적기인다태성여대장암역감성명현상관.
Objective To investigate the relationship between genetic polymorphism in prmotor 1b T-129C,exon 21 G2 677T/A and exon 26 C3 435T of the multidrug resistance 1 (MDR1) gene and the risk of sporadic colorectal carcinoma in Hubei Han population of China.Methods A total of 133 patients with colorectal carcinoma and 386 healthy subjects as controls were included.Snapshot single nucleotide polymorphism (SNP) typing was used to determine the allele and genotype frequencies of MDR1 T-129C,C3 435T,and G2 677T/A.Results There was significant difference in the distribution of genotype of MDR1 T-129C between the control group and case group.The mutant heterozygous TC genotype was increased significantly in the case guoup (12.8%) as compared with that in the control group (6.3%),and was associated with occurrence of colorectal carcinoma [odds ratio (OR) =2.186,P<0.05].The frequency of-129C allele was increased significantly in the case group as compared with that in the control group (P < 0.05).The data showed the evidence of pairwise linkage disequilibrium among the three common SNPs (T-129C-G2 677T/A-C3 435T).The haplotypes of TGC (40.27%),TTT (36.96%),TAC (9.97%) and TTC (5.02%) were predominant.The haplotype CGC and CTT distributed significantly differently between the case group (1.98% and 1.25%) and control group (0.51% and 0.17%,P < 0.05).The frequency of the haplotype TGC/TTT in the colon carcinoma group (35.5%) was higher than in the rectal carcinoma group (15.5%,P < 0.05).Conclusion The present findings suggest that T-129C polymorphism in MDR1 gene may be a genetic susceptibility factor for sporadic colorectal carcinoma in Hubei Han population of China.