中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
4期
792-794
,共3页
赵佳%刘东雷%杨洋%吴恺%赵松
趙佳%劉東雷%楊洋%吳愷%趙鬆
조가%류동뢰%양양%오개%조송
丙泊酚%肺癌%脱噬作用%水通道蛋白1
丙泊酚%肺癌%脫噬作用%水通道蛋白1
병박분%폐암%탈서작용%수통도단백1
Propofol%Lung cancer%Apoptosis%Aquaporin 1
目的 探讨丙泊酚对肺癌A549细胞凋亡的影响及其机制.方法 用噻唑蓝(MTT)法检测丙泊酚对肺癌A549细胞增殖作用的影响,采用流式细胞技术检测丙泊酚对A549细胞细胞凋亡的影响,Western blot测定丙泊酚对A549细胞水通道蛋白1(AQP1)表达的影响,应用Transwell小室实验检测AQP1蛋白表达对肺癌A549细胞迁移的影响.结果 MTT法和流式细胞法显示不同浓度丙泊酚作用A549细胞株48h后,细胞抑制率和凋亡率分别为(52.8±5.2)％、(61.2±5.9)％、(69.4±6.1)％;(42.8±4.1)％、(59.8±11.2)％、(71.4±13.4)％.Western blot结果显示不同浓度丙泊酚作用A549细胞48h后,AQP1蛋白的表达水平为0.87±0.12、0.54±0.07、0.31 ±0.04;ranswell小室实验显示不同浓度丙泊酚作用于A549细胞后,迁移细胞数分别为(61.35±8.98)、(51.78±7.58)、(40.35±5.12)个.结论 丙泊酚对人肺癌A549细胞的生长有明显的抑制作用,诱导肿瘤细胞凋亡,并能抑制肺腺癌细胞的迁移能力,其机制可能与下调AQP1蛋白表达有关.
目的 探討丙泊酚對肺癌A549細胞凋亡的影響及其機製.方法 用噻唑藍(MTT)法檢測丙泊酚對肺癌A549細胞增殖作用的影響,採用流式細胞技術檢測丙泊酚對A549細胞細胞凋亡的影響,Western blot測定丙泊酚對A549細胞水通道蛋白1(AQP1)錶達的影響,應用Transwell小室實驗檢測AQP1蛋白錶達對肺癌A549細胞遷移的影響.結果 MTT法和流式細胞法顯示不同濃度丙泊酚作用A549細胞株48h後,細胞抑製率和凋亡率分彆為(52.8±5.2)％、(61.2±5.9)％、(69.4±6.1)％;(42.8±4.1)％、(59.8±11.2)％、(71.4±13.4)％.Western blot結果顯示不同濃度丙泊酚作用A549細胞48h後,AQP1蛋白的錶達水平為0.87±0.12、0.54±0.07、0.31 ±0.04;ranswell小室實驗顯示不同濃度丙泊酚作用于A549細胞後,遷移細胞數分彆為(61.35±8.98)、(51.78±7.58)、(40.35±5.12)箇.結論 丙泊酚對人肺癌A549細胞的生長有明顯的抑製作用,誘導腫瘤細胞凋亡,併能抑製肺腺癌細胞的遷移能力,其機製可能與下調AQP1蛋白錶達有關.
목적 탐토병박분대폐암A549세포조망적영향급기궤제.방법 용새서람(MTT)법검측병박분대폐암A549세포증식작용적영향,채용류식세포기술검측병박분대A549세포세포조망적영향,Western blot측정병박분대A549세포수통도단백1(AQP1)표체적영향,응용Transwell소실실험검측AQP1단백표체대폐암A549세포천이적영향.결과 MTT법화류식세포법현시불동농도병박분작용A549세포주48h후,세포억제솔화조망솔분별위(52.8±5.2)％、(61.2±5.9)％、(69.4±6.1)％;(42.8±4.1)％、(59.8±11.2)％、(71.4±13.4)％.Western blot결과현시불동농도병박분작용A549세포48h후,AQP1단백적표체수평위0.87±0.12、0.54±0.07、0.31 ±0.04;ranswell소실실험현시불동농도병박분작용우A549세포후,천이세포수분별위(61.35±8.98)、(51.78±7.58)、(40.35±5.12)개.결론 병박분대인폐암A549세포적생장유명현적억제작용,유도종류세포조망,병능억제폐선암세포적천이능력,기궤제가능여하조AQP1단백표체유관.
Objective To investigate the influence of propofol on lung cancer cell proliferation and apoptosisin vitro.Methods MTT assay was used to detect the inhibition effect of propofol on the growth of A549.Flow cytometry was applied to detect the impact of propofol on apoptosis of A549 cells.Western blotting was used to determine the effect of propofol on aquaporin 1 (AQP1) protein expression in A549 cells,and the migration ability of A549 cells was investigated by Transwell chamber.Results MTT assay and flow cytometry showed that the rate of inhibition and apoptosis in the experiment group after propofol actingon A549 were (52.8±5.2)％,(61.2 ±5.9)％,(69.4 ±6.1)％ ; (42.8 ±4.1)％,(59.8 ± 11.2)％,(71.4 ± 13.4)％,respectively.The expression of AQP1 protein was 0.87 ± 0.12、0.54 ± 0.07、0.31 ± 0.04; and the migration ability of A549 cells was 1.35 ± 8.98、51.78 ± 7.58、40.35 ± 5.12.Conclusion Propofol can significantly inhibit the proliferation,apoptosis and migration ability of human lung cancer A549 cells,which may be associated with the down-regulation of AQP1 protein.
