中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
4期
781-783
,共3页
李晓燕%李林蔚%王文玉%高天慧%周云
李曉燕%李林蔚%王文玉%高天慧%週雲
리효연%리림위%왕문옥%고천혜%주운
食管癌相关基因4%TMPRSS11A%食管癌
食管癌相關基因4%TMPRSS11A%食管癌
식관암상관기인4%TMPRSS11A%식관암
Esophageal cancer related gene 4%TMPRSS11A%Esophageal cancer
目的 探讨食管癌相关基因4 (ECRG4)蛋白在食管癌中的抑癌功能和作用机制.方法 利用Western blot方法和共聚焦显微成像分析验证ECRG4蛋白是分泌蛋白;利用细胞增殖曲线分析检测ECRG4蛋白的抑癌功能;利用亲和结合实验检测ECRG4蛋白和TMPRSS11A的相互作用.结果 ECRG4蛋白是小分子分泌蛋白;浓度为10 mg/L的重组ECRG4蛋白抑制食管癌细胞增殖,第5天的抑制率为37.5％(P＜0.05);ECRG4蛋白和TMPRSS11A体外存在相互作用.结论 ECRG4蛋白和TMPRSS11A相互作用抑制食管癌细胞增殖.
目的 探討食管癌相關基因4 (ECRG4)蛋白在食管癌中的抑癌功能和作用機製.方法 利用Western blot方法和共聚焦顯微成像分析驗證ECRG4蛋白是分泌蛋白;利用細胞增殖麯線分析檢測ECRG4蛋白的抑癌功能;利用親和結閤實驗檢測ECRG4蛋白和TMPRSS11A的相互作用.結果 ECRG4蛋白是小分子分泌蛋白;濃度為10 mg/L的重組ECRG4蛋白抑製食管癌細胞增殖,第5天的抑製率為37.5％(P＜0.05);ECRG4蛋白和TMPRSS11A體外存在相互作用.結論 ECRG4蛋白和TMPRSS11A相互作用抑製食管癌細胞增殖.
목적 탐토식관암상관기인4 (ECRG4)단백재식관암중적억암공능화작용궤제.방법 이용Western blot방법화공취초현미성상분석험증ECRG4단백시분비단백;이용세포증식곡선분석검측ECRG4단백적억암공능;이용친화결합실험검측ECRG4단백화TMPRSS11A적상호작용.결과 ECRG4단백시소분자분비단백;농도위10 mg/L적중조ECRG4단백억제식관암세포증식,제5천적억제솔위37.5％(P＜0.05);ECRG4단백화TMPRSS11A체외존재상호작용.결론 ECRG4단백화TMPRSS11A상호작용억제식관암세포증식.
Objective To investigate the tumor-suppressing function and molecular mechanism of esophageal cancer related gene 4 (ECRG4) protein in esophageal carcinoma.Methods Western blotting and confocal microscopy were used to verify that ECRG4 protein was secreted protein.Cell proliferation curve assay was utilized to examine the tumor-suppressing function of ECRG4 protein.Binding affinity assay was used to study the protein interactions between ECRG4 and TMPRSS11A.Results ECRG4 protein was secreted small-molecular protein.Moreover,the recombinant ECRG4 protein in 10 mg/L concentration could inhibit esophageal cancer cells proliferation,with the inhibition rate at the fifth day being 37.5％,as compared with phosphate buffer (PBS) control group (P ＜ 0.05).Furthermore,ECRG4 protein interacted with TMPRSS11A in vitro.Conclusion ECRG4 protein interacted with TMPRSS11A to inhibit esophageal cancer cells proliferation.
