CAJ | 학술논문

目的观察右旋美托咪啶对内毒素休克大鼠海马炎性反应和认知功能损伤的影响.方法 60只雄性SD大鼠随机分为4组:生理盐水对照组、内毒素休克组、高剂量右旋美托咪啶+内毒素血症组(High-dose Dex组)和低剂量右旋美托咪啶+内毒素血症组(Low-dose Dex组).酶联免疫吸附试验(ELISA)法检测海马白细胞介素(IL)-6及肿瘤坏死因子-α(TNF-α)表达水平.Western blot法检测海马中凋亡标志物活化半胱氨酰天冬氨酸特异性蛋白酶(Caspase)-3的表达水平.Morris水迷宫法测定认知功能.结果每小时2.0μg/kg和0.5μg/kg右旋美托咪啶分别使内毒素休克大鼠海马IL-6表达下降67.1％和36.5％ (P＜0.05),TNF-α表达下降56.7％和53.1％(P＜0.05),活化Caspase-3水平下降32.5％和38.5％ (P ＜0.05),并缓解内毒素休克大鼠的认知功能障碍(P＜0.05).结论右旋美托咪啶可缓解内毒素休克大鼠海马炎性反应并减轻内毒素休克导致的认知功能损伤.
목적 관찰우선미탁미정대내독소휴극대서해마염성반응화인지공능손상적영향.방법 60지웅성SD대서수궤분위4조:생리염수대조조、내독소휴극조、고제량우선미탁미정+내독소혈증조(High-dose Dex조)화저제량우선미탁미정+내독소혈증조(Low-dose Dex조).매련면역흡부시험(ELISA)법검측해마백세포개소(IL)-6급종류배사인자-α(TNF-α)표체수평.Western blot법검측해마중조망표지물활화반광안선천동안산특이성단백매(Caspase)-3적표체수평.Morris수미궁법측정인지공능.결과 매소시2.0μg/kg화0.5μg/kg우선미탁미정분별사내독소휴극대서해마IL-6표체하강67.1％화36.5％ (P＜0.05),TNF-α표체하강56.7％화53.1％(P＜0.05),활화Caspase-3수평하강32.5％화38.5％ (P ＜0.05),병완해내독소휴극대서적인지공능장애(P＜0.05).결론 우선미탁미정가완해내독소휴극대서해마염성반응병감경내독소휴극도치적인지공능손상.
Objective To investigate the effects of dexmedetomidine (Dex) on hippocampal inflammatory responses and cognitive impairment in rats with endotoxin-induced shock.Methods Sixty male SD rats were randomly divided into 4 groups including saline control group,high-dose Dex group and low-dose Dex group.Hippocampal protein levels of interleukin (IL)-6 and tumor necrosis factor-α (TNF-α) were detected by enzyme linked immunosorbent assay (ELISA).Hippocampal active Caspase -3 protein expression levels were examined by Western blotting.Results As compared with saline control group,2.0 μg/(kg· h) and 0.5 μg/(kg·h) Dex respectively decreased hippocampal IL-6 protein levels by 67.1％ and 36.5％ (P ＜ 0.05),and decreased hippocampal TNF-α protein levels by 56.7％ and 53.1％ (P ＜ 0.05),reduced hippocampal active Caspase-3 levels by 32.5％ and 38.5％ (P ＜ 0.05) and then attenuated LPS-induced cognitive impairment (P ＜ 0.05).Conclusion Dex can reduce hippocampal inflammatory responses and attenuate cognitive impairment caused by endotoxin-induced shock.