中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
4期
867-869
,共3页
武干生%吴树彪%胡盼盼%董铁立
武榦生%吳樹彪%鬍盼盼%董鐵立
무간생%오수표%호반반%동철립
神经病理性痛%组蛋白乙酰转移酶P300%CREB结合蛋白
神經病理性痛%組蛋白乙酰轉移酶P300%CREB結閤蛋白
신경병이성통%조단백을선전이매P300%CREB결합단백
Neuropathic pain%P300%CREB binding protein
目的 观察坐骨神经慢性压榨损伤(CCI)模型大鼠脊髓灰质后角组蛋白乙酰转移酶P300与CREB结合蛋白(CBP)表达的变化及其细胞分布.方法 将雄性SD大鼠48只随机分为空白组(12只)、假手术组(12只)、CCI造模组(24只).建模成功后,应用免疫组织化学法及免疫荧光法观察P300及CBP的表达及定位.结果 与空白组和假手术组比较,CCI模型组大鼠脊髓灰质后角细胞核内P300/CBP表达量上升(P＞0.05),并伴有机械痛与热痛行为学特征,且于造模后14d达到峰值(P300数值为478.35±22.61,CPB为891.55±32.19),采用免疫荧光法观察与其共定位的细胞为神经元细胞.结论 P300/CBP与神经病理性痛的发生有关,且在疾病发展过程中仅分布于脊髓灰质后角的神经元细胞内.
目的 觀察坐骨神經慢性壓榨損傷(CCI)模型大鼠脊髓灰質後角組蛋白乙酰轉移酶P300與CREB結閤蛋白(CBP)錶達的變化及其細胞分佈.方法 將雄性SD大鼠48隻隨機分為空白組(12隻)、假手術組(12隻)、CCI造模組(24隻).建模成功後,應用免疫組織化學法及免疫熒光法觀察P300及CBP的錶達及定位.結果 與空白組和假手術組比較,CCI模型組大鼠脊髓灰質後角細胞覈內P300/CBP錶達量上升(P＞0.05),併伴有機械痛與熱痛行為學特徵,且于造模後14d達到峰值(P300數值為478.35±22.61,CPB為891.55±32.19),採用免疫熒光法觀察與其共定位的細胞為神經元細胞.結論 P300/CBP與神經病理性痛的髮生有關,且在疾病髮展過程中僅分佈于脊髓灰質後角的神經元細胞內.
목적 관찰좌골신경만성압자손상(CCI)모형대서척수회질후각조단백을선전이매P300여CREB결합단백(CBP)표체적변화급기세포분포.방법 장웅성SD대서48지수궤분위공백조(12지)、가수술조(12지)、CCI조모조(24지).건모성공후,응용면역조직화학법급면역형광법관찰P300급CBP적표체급정위.결과 여공백조화가수술조비교,CCI모형조대서척수회질후각세포핵내P300/CBP표체량상승(P＞0.05),병반유궤계통여열통행위학특정,차우조모후14d체도봉치(P300수치위478.35±22.61,CPB위891.55±32.19),채용면역형광법관찰여기공정위적세포위신경원세포.결론 P300/CBP여신경병이성통적발생유관,차재질병발전과정중부분포우척수회질후각적신경원세포내.
Objective To investigate the distribution and dynamic changes of P300/CREB binding protein (CBP) expression in the spinal cord in the rat chronic constrictive injury (CCI) model.Methods Thermal withdrawal latency and mechanical withdrawal threshold were used to evaluate the CCI surgery.The number of IR cells was showed increased P300 and CBP immunoreactivity (IR) compared with normal control and sham-operated rats.Cell-type determination by immunofluorescence at 14 days.Results P300 and CBP expressed predominantly in nuclei in the gray matter of rat spinal cord.Rats undergoing CCI surgery showed increased IR of P300 and CBP compared with normal control and sham-operated rats(P ＞0.05).The number of IR cells reached the peak at 14 days following CCI compared with those on 3,7,and 21 days,accompanied with significant behavioral changes of neuropathic pain.Cell-type determination by immunofluorescence at 14 days following CCI revealed that P300 and CBP expressed in neurons.Conclusion These results suggest that P300 and CBP are probably involved in the maintenance of neuropathic pain on spinal cord level.Furthermore,P300 and CBP may serve as a sensor only in neurons in the adult rat spinal cord.
