CAJ | 학술논문

目的构建稳定的SD大鼠脊髓损伤模型,探讨早期联合应用骨髓间充质干细胞(BMSCs)和促红细胞生成素(EPO)对其修复的作用机制.方法采用Hatteras Instruments PCI3000精密打击器,将120只SD雄性大鼠建立脊髓损伤模型并完全随机分为4组,A组为损伤对照组,B组为EPO治疗组,C组为BMSCs治疗组,D组为BMSCs联合EPO治疗组.术后1、3、7、14、28d每组分别取6只大鼠,观察Basso-Beattie-Bresnahan(BBB)运动评分、细胞凋亡指数和核因子(NF)-200的表达,其中C组和D组加测5-溴脱氧尿嘧啶核苷(BrdU)标记的BMSCs在损伤区的阳性率.结果术后7、14、28 d,B、C、D组BBB运动功能评分高于A组,D组高于B、C组,差异有统计学意义(P＜0.05).术后1d损伤区细胞明显凋亡,3d达到高峰,以后逐渐下降,细胞凋亡指数在各时间段内组间比较均为:A＞B＞C＞D,差异有统计学意义(P＜0.05).NF-200表达水平于术后迅速下降,3d以后逐渐升高,各时间段内组间比较均为:A＜B＜C＜D,差异有统计学意义(P＜0.05).BrdU标记的BMSCs阳性率在术后各时间段D组均高于C组,差异有统计学意义(P＜0.05).结论大鼠BMSCs和EPO早期联合应用可通过减少细胞凋亡、促进神经蛋白表达对损伤组织起保护和修复作用,提高疗效.
목적 구건은정적SD대서척수손상모형,탐토조기연합응용골수간충질간세포(BMSCs)화촉홍세포생성소(EPO)대기수복적작용궤제.방법 채용Hatteras Instruments PCI3000정밀타격기,장120지SD웅성대서건립척수손상모형병완전수궤분위4조,A조위손상대조조,B조위EPO치료조,C조위BMSCs치료조,D조위BMSCs연합EPO치료조.술후1、3、7、14、28d매조분별취6지대서,관찰Basso-Beattie-Bresnahan(BBB)운동평분、세포조망지수화핵인자(NF)-200적표체,기중C조화D조가측5-추탈양뇨밀정핵감(BrdU)표기적BMSCs재손상구적양성솔.결과 술후7、14、28 d,B、C、D조BBB운동공능평분고우A조,D조고우B、C조,차이유통계학의의(P＜0.05).술후1d손상구세포명현조망,3d체도고봉,이후축점하강,세포조망지수재각시간단내조간비교균위:A＞B＞C＞D,차이유통계학의의(P＜0.05).NF-200표체수평우술후신속하강,3d이후축점승고,각시간단내조간비교균위:A＜B＜C＜D,차이유통계학의의(P＜0.05).BrdU표기적BMSCs양성솔재술후각시간단D조균고우C조,차이유통계학의의(P＜0.05).결론 대서BMSCs화EPO조기연합응용가통과감소세포조망、촉진신경단백표체대손상조직기보호화수복작용,제고료효.
Objective The study aims to establish the steady and easily replicated models of spinal cord injury in SD rats,and to investigate the effect of bone marrow mesenchymal stem cells transplantation combined with erythropoietin for repairing spinal cord injury.Methods Models of spinal cord injury were established by Hatteras Instruments PCI3000 Precision Cortical Inpactor.120 SD rats were randomly divided into four groups:control group (group A,n =30),erythropoietin (EPO) group (group B,n =30),bone marrow-derived mesenchymal stem cells (BMSCs) group (group C,n =30),BMSCs and EPO associated group (group D,n =30),Six rats in each group were scored by the Basso-Beattie-Bresnahan (BBB) locomotor rating scale to evaluate motor function on the 1th,3th,7th,14th,28th day after spinal cord injury,then rats were killed to observe cell apoptosis and the expression of nuclear factor (NF)-200,what's more,bromodeoxyuridine (BrdU)-positive BMSCs were examined in group C and group D.The data was analyzed by SPSS 19.0.Results The BBB scores were descendingly ordered as group D,B,C and A at 7th,14th,28thday after spinal cord injury (P ＜0.05).The apoptotic cells index began to rise with 24 hours and reached its climaxuntil 3rd day after spinal cord injury before declining,Groups comparison of AI in each time was for A ＞ B ＞ C ＞ D (P ＜ 0.05).NF-200 expression decreased sharply after spinal cord injury and rebounded to rise 3 days later.Groups comparison of optical density value in each time was for A ＜ B ＜ C ＜ D (P ＜ 0.05).The BrdU-positive rate of group D was higher than that of group C (P ＜ 0.05).Conclusion BMSCs cooperates with EPO could improve protection and restoration of spinal cord injury by reducing apoptosis,accelerating neuro-protein expression.