CAJ | 학술논문

目的观察不同剂量X线照射成骨细胞后,细胞形态、胞内微结构及纤维肌动蛋白的变化.方法采用医用直线加速器以0、0.5、5.0Gy作用成骨细胞(MC3T3-E1)后,用倒置相差显微镜观察细胞形态变化,透射电镜观察细胞内微结构变化以及异硫酸氢荧光素-鬼笔环肽(FITC-phalloidin)对各实验组细胞的纤维肌动蛋白(F-actin)进行染色,荧光显微镜下观察各实验组F-actin细胞骨架的变化.结果 X线照射后2h,0.5、5.0Gy组细胞F-actin绿色荧光强度明显低于未照射组(25.329±12.209、27.021±13.049比29.107±13.296),差异有统计学意义(P＜0.05).但24h后0.5、5.0Gy组细胞肌动蛋白骨架发生重构,F-actin染色逐渐增强、纤维增粗,照射后第3天时最为显著(38.687±18.072、36.039±12.128比35.645±17.213),至第5天时F-actin染色逐渐恢复正常,与0Gy组接近(28.527±14.107、27.258±13.322比27.309±15.039).结论 X线辐射短时间内能使成骨细胞骨架破坏、F-actin解聚,但随后细胞肌动蛋白骨架发生重构,F-actin表达增强,至第5天时逐渐恢复正常.
목적 관찰불동제량X선조사성골세포후,세포형태、포내미결구급섬유기동단백적변화.방법 채용의용직선가속기이0、0.5、5.0Gy작용성골세포(MC3T3-E1)후,용도치상차현미경관찰세포형태변화,투사전경관찰세포내미결구변화이급이류산경형광소-귀필배태(FITC-phalloidin)대각실험조세포적섬유기동단백(F-actin)진행염색,형광현미경하관찰각실험조F-actin세포골가적변화.결과 X선조사후2h,0.5、5.0Gy조세포F-actin록색형광강도명현저우미조사조(25.329±12.209、27.021±13.049비29.107±13.296),차이유통계학의의(P＜0.05).단24h후0.5、5.0Gy조세포기동단백골가발생중구,F-actin염색축점증강、섬유증조,조사후제3천시최위현저(38.687±18.072、36.039±12.128비35.645±17.213),지제5천시F-actin염색축점회복정상,여0Gy조접근(28.527±14.107、27.258±13.322비27.309±15.039).결론 X선복사단시간내능사성골세포골가파배、F-actin해취,단수후세포기동단백골가발생중구,F-actin표체증강,지제5천시축점회복정상.
Objective To observe the effects of different doses of X-ray irradiation on the morphology,mierostructure changes and actin cytoskeleton of osteoblasts.The findings of this research will provide evidence for further study of low dose X-ray irradiation biological effects.Methods MC3T3-E1 cells were exposed to irradiation of 0.5,5.0 Gy.We investigated cellular morphological changes by phase contrast microscope and transmission electron microscopy.The organization of actin microfilaments was determined by immunofluorescence.Results After 2 h exposure to irradiation,the F-actin fluorescence intensity of ceils in 0.5,5.0 Gy group were significantly lower than non-irradiated group (25.329 ± 12.209,27.021 ± 13.049 vs.29.107 ± 13.296,P ＜ 0.05).But 24 hours later,the fluorescence intensity of F-actin in 0.5,5.0 Gy group increased gradually and the fiber stress also increased.The most significant changes appeared in the third day after X-ray irrddiation (38.687 ± 18.072,36.039 ± 12.128 vs.35.645 ± 17.213).However,these changes gradually returned to normal in the fifth day,close to 0 Gy group (28.527 ±14.107,27.258 ±13.322 vs.27.309±15.039).Conclusion Thecytoskeleton of MC3TE-E cells were destroyed after 2 hours,exposure to X-ray irradiation.Howerver,0.5,5.0 Gy X-ray irradiation induced reorganization of actin filaments of MC3T3 cells 1 d later.