CAJ | 학술논문

目的建立模拟急性缺血性卒中血管内机械再通治疗后颅内出血转化(-HT)的动物模型.方法通过诱发高糖血症联合线栓法闭塞大鼠大脑中动脉5h再通的方法,观察血管再通19 h后大鼠HT的情况.结果 24只模型组大鼠在基底节和皮层出现不同程度HT,以脑实质血肿为主.联合多次高糖注射比单次高糖注射更易形成HT[88.5 (23/26)比25.0(1/4),P＜0.05].血管再通后闭塞侧血脑屏障破坏,脑组织中基质金属蛋白酶(MMP)-9蛋白较假手术组明显表达(0.65±0.06比0.37±0.13,P＜0.01),而基底膜胶原酶(Collagen)Ⅳ表达较假手术组减少(0.48 ±0.10比0.84±0.08,P＜0.01).结论多次高糖注射诱发大鼠高糖血症联合线栓法闭塞大脑中动脉5h后再通的方法可成功模拟血管内机械再通治疗后HT的发生.
목적 건립모의급성결혈성졸중혈관내궤계재통치료후로내출혈전화(-HT)적동물모형.방법 통과유발고당혈증연합선전법폐새대서대뇌중동맥5h재통적방법,관찰혈관재통19 h후대서HT적정황.결과 24지모형조대서재기저절화피층출현불동정도HT,이뇌실질혈종위주.연합다차고당주사비단차고당주사경역형성HT[88.5 (23/26)비25.0(1/4),P＜0.05].혈관재통후폐새측혈뇌병장파배,뇌조직중기질금속단백매(MMP)-9단백교가수술조명현표체(0.65±0.06비0.37±0.13,P＜0.01),이기저막효원매(Collagen)Ⅳ표체교가수술조감소(0.48 ±0.10비0.84±0.08,P＜0.01).결론 다차고당주사유발대서고당혈증연합선전법폐새대뇌중동맥5h후재통적방법가성공모의혈관내궤계재통치료후HT적발생.
Objective To investigate an ideal animal model for hemorrhagic transformation (HT) after mechanical thrombectomy for acute ischemic stroke with large-vessel occlusion.Methods Hemorrhagic transformation was examined by 5 h transient middle cerebral artery occlusion and 19 h reperfusion combined with hyperglycemia induced by injection of 50％ glucose.Results We found that 80％ animals in the experimental group had HT in the basal ganglia and cerebral cortex.Most of HT was the kind of parenchymal hematoma.HT occurred more frequently in the group of multiple injection of high glucose than in the group of single injection of high glucose [88.5 ％ (23/26) vs.25.0％ (1/4),P ＜ 0.05].Blood-brain barrier permeability was increased in the ischemic-reperfusion hemisphere.The expression of matrix metalloproteinase-9 was increased in the ipsilateral hemisphere (0.65 ± 0.06 vs.0.37 ± 0.13,P ＜ 0.01),and that of collagen type Ⅳ decreased (0.48 ±0.10 vs.0.84 ±0.08,P＜0.01).Conclusion HT following endovascular mechanical thrombectomy can be successfully simulated by transient middle cerebral artery occlusion for 5 h combined with hyperglycemia induced by multiple injection of high glucose in rats.