CAJ | 학술논문

目的探讨食管鳞癌患者手术前后血清p53抗体动态变化规律及其与临床病理特征之间的关系.方法选取因食管鳞癌行食管癌根治术患者98例,其中男68例,女30例,对照组为门诊健康体检者30例,采用酶联免疫吸附法(ELISA)分别于术前1d、术后第7、30、90、180天检测食管癌患者血清p53抗体,并与患者临床病理特征相结合进行研究.结果 (1)食管癌患者血清p53抗体浓度[(338.96±104.14) ng/L比(242.30±39.79) ng/L]和阳性率(37.8％比0)均明显高于对照组(P＜0.05).(2)食管癌患者血清p53抗体阳性率与患者性别、年龄、肿瘤体积无关(P＞0.05),而与患者吸烟量、肿瘤组织分化程度、TNM分期呈正相关(P＜0.05).(3)在行食管癌根治性切除术后,食管癌患者血清p53抗体浓度呈下降趋势,术后第30天基本降至正常水平.结论 (1)血清p53抗体可以作为食管鳞癌诊断和判断其预后的潜在标志物.(2)监测食管鳞癌患者术后的血清p53抗体水平对早期发现其复发或转移有重要的临床参考价值.
목적 탐토식관린암환자수술전후혈청p53항체동태변화규률급기여림상병리특정지간적관계.방법 선취인식관린암행식관암근치술환자98례,기중남68례,녀30례,대조조위문진건강체검자30례,채용매련면역흡부법(ELISA)분별우술전1d、술후제7、30、90、180천검측식관암환자혈청p53항체,병여환자림상병리특정상결합진행연구.결과 (1)식관암환자혈청p53항체농도[(338.96±104.14) ng/L비(242.30±39.79) ng/L]화양성솔(37.8％비0)균명현고우대조조(P＜0.05).(2)식관암환자혈청p53항체양성솔여환자성별、년령、종류체적무관(P＞0.05),이여환자흡연량、종류조직분화정도、TNM분기정정상관(P＜0.05).(3)재행식관암근치성절제술후,식관암환자혈청p53항체농도정하강추세,술후제30천기본강지정상수평.결론 (1)혈청p53항체가이작위식관린암진단화판단기예후적잠재표지물.(2)감측식관린암환자술후적혈청p53항체수평대조기발현기복발혹전이유중요적림상삼고개치.
Objective To explore the relationship between serum p53 antibodies and clinicopathological characteristics in patients with esophageal squamous cell carcinoma (ESCC) and to investigate sequential changing regularity of serum p53 antibodies before and after surgical resection.Methods The eligible ESCC cases of this study were consecutively recruited between October 2010 and February 2012 from Luhe Hospital.There were 68 males and 30 females.The serum p53 antibody concentration was detected in ESCC patients and in 30 healthy adults as controls by enzyme linked immunosorbent assay (ELISA).The blood samples were collected on the day before operation and on the 7th,30th,90th and 180th days postoperatively.Results (1) The concentration [(338.96 + 104.14) ng/L vs.(242.30 ±39.79) ng/L] and positive rate (37.8％ vs.0) of serum p53 antibodies in patients with ESCC were significantly higher than normal individuals (P ＜ 0.05).(2) The positive rate of pre-operative serum p53 antibodies in patients with ESCC positively correlated with the pieces of consumed cigarette,cell differentiation and TNM (P ＜ 0.05).(3) The level of serum p53 antibodies decreased gradually in patients with ESCC underwent radical resection of the cancer,and completely returned to the normal level around the 30th day after operation.Conclusion (1) Serum p53 antibody can be used as a potential marker for early detection of ESCC and predication of prognosis.(2) It has important clinical reference value for detecting the early recurrence or metastasis to monitor serum p53 antibodies level on postoperative patients with ESCC.