江西中医药大学学报
江西中醫藥大學學報
강서중의약대학학보
Journal of Jiangxi University of Traditional Chinese Medicine
2015年
2期
100-102,105
,共4页
一测多评%校正因子%高效液相色谱%人参皂苷%参麦注射液
一測多評%校正因子%高效液相色譜%人參皂苷%參麥註射液
일측다평%교정인자%고효액상색보%인삼조감%삼맥주사액
Quantitative analysis multi -components by single marker(QMAS)%Relative correction factor%HPLC%Ginsenoside%Shenmai injection
目的：应用一测多评法建立同时测定参麦注射液7个成分的HPLC含量测定。方法：以人参皂苷Rg1作内参物，计算人参皂苷Re、Rf、Rb1、Rc、Rb2、Rd的相对校正因子。利用相对校正因子，计算参麦注射液中各成分的含量。比较一测多评法与外标法测定结果的差异，以验证一测多评法的适用性。结果：各成分相对校正因子重现性良好。人参皂苷Re、Rf、Rb1、Rc、Rb2、Rd按一测多评方法与外标法测定结果无显著差异。结论：一测多评法可以作为一种简便准确的质量评价模式用于参麦注射液多种人参皂苷类成分的含量测定。
目的：應用一測多評法建立同時測定參麥註射液7箇成分的HPLC含量測定。方法：以人參皂苷Rg1作內參物，計算人參皂苷Re、Rf、Rb1、Rc、Rb2、Rd的相對校正因子。利用相對校正因子，計算參麥註射液中各成分的含量。比較一測多評法與外標法測定結果的差異，以驗證一測多評法的適用性。結果：各成分相對校正因子重現性良好。人參皂苷Re、Rf、Rb1、Rc、Rb2、Rd按一測多評方法與外標法測定結果無顯著差異。結論：一測多評法可以作為一種簡便準確的質量評價模式用于參麥註射液多種人參皂苷類成分的含量測定。
목적：응용일측다평법건립동시측정삼맥주사액7개성분적HPLC함량측정。방법：이인삼조감Rg1작내삼물，계산인삼조감Re、Rf、Rb1、Rc、Rb2、Rd적상대교정인자。이용상대교정인자，계산삼맥주사액중각성분적함량。비교일측다평법여외표법측정결과적차이，이험증일측다평법적괄용성。결과：각성분상대교정인자중현성량호。인삼조감Re、Rf、Rb1、Rc、Rb2、Rd안일측다평방법여외표법측정결과무현저차이。결론：일측다평법가이작위일충간편준학적질량평개모식용우삼맥주사액다충인삼조감류성분적함량측정。
Objective:To establish a new quality evaluation method for Shenmai injection,the content of seven components being calcu-lated by using quantitative analysis of multi-components by single-marker( QAMS) of HPLC.Methods:With ginsenoside Rg1 as in-ternal material,the relative correction factor(RCF) of ginsenoside Re,Rf,Rb1,Rc,Rb2,Rd were evaluated.The content of each com-ponent in the Shenmai injection were calculated by itsˊRCFs.The QAMS method and external standard method were compared to deter-mine differences in results, to verify the applicability of QAMS method.Results:The RCFs showed good reproducibility in a certain ranges.For all components, there was no significant difference between the quantitative results of the two methods.Conclusion:The QAMS method is feasible and credible, and could be used to determine the multiple components of ginsenoside in Shenmai injection.
