CAJ | 학술논문

目的：通过短期给予新生幼鼠不同抗癫痫药，观察苯巴比妥（ PB）和托吡酯（ TPM）对幼鼠远期学习记忆功能的影响及刺五加的保护作用。方法实验动物分两批：第1批3窝健康3日龄Sprague－Dawley（SD）大鼠30只（雌、雄不分）随机分为3组，生理盐水对照组10只、苯巴比妥组10只、苯巴比妥＋刺五加组10只；第2批3窝健康3日龄Sprague－Dawley（SD）大鼠30只（雌、雄不分）随机分为3组，生理盐水对照组10只，托吡酯组10只，托吡酯＋刺五加组10只。对照组给予生理盐水10mL? kg－1? d－1，苯巴比妥组：苯巴比妥62．5mg? kg－1? d－1，苯巴比妥＋刺五加组：苯巴比妥62．5mg? kg－1? d－1，刺五加干粉5．6g? kg－1? d－1。托吡酯组：40mg? kg－1? d－1，托吡酯＋刺五加组：托吡酯40mg? kg－1? d－1，刺五加干粉5．6g? kg－1? d－1。每日称重后腹腔注射，连续3天。正常饲养至1月龄，最后各组选取8只大鼠，参加水迷宫测试。结果苯巴比妥干预组大鼠寻找水下平台的潜伏期较对照组明显延长，均存在显著性差异（t＝－3．542，P＝0．005）；穿越有效区次数较对照组减少（t＝3．352，P＝0．005）。加用刺五加保护后，与对照组比较，潜伏期（t＝－1．313，P＝0．210）及穿越有效区次数（t＝0．051，P＝0．128）均无显著性差异。托吡酯干预组大鼠与对照组比较潜伏期（t＝－1．920，P＝0．075）、穿越有效区次数（t＝1．915，P＝0．076）；托吡酯组与托吡酯＋刺五加组比较潜伏期（t＝－0．597，P＝0．560）、穿越有效区次数（t＝1．660，P＝0．119），均无显著性差异。结论未成熟脑短期应用苯巴比妥损害远期学习记忆，刺五加具有保护作用，短期应用中等剂量的托吡酯不会对未成熟脑产生远期学习记忆障碍。
목적：통과단기급여신생유서불동항전간약，관찰분파비타（ PB）화탁필지（ TPM）대유서원기학습기억공능적영향급자오가적보호작용。방법실험동물분량비：제1비3와건강3일령Sprague－Dawley（SD）대서30지（자、웅불분）수궤분위3조，생리염수대조조10지、분파비타조10지、분파비타＋자오가조10지；제2비3와건강3일령Sprague－Dawley（SD）대서30지（자、웅불분）수궤분위3조，생리염수대조조10지，탁필지조10지，탁필지＋자오가조10지。대조조급여생리염수10mL? kg－1? d－1，분파비타조：분파비타62．5mg? kg－1? d－1，분파비타＋자오가조：분파비타62．5mg? kg－1? d－1，자오가간분5．6g? kg－1? d－1。탁필지조：40mg? kg－1? d－1，탁필지＋자오가조：탁필지40mg? kg－1? d－1，자오가간분5．6g? kg－1? d－1。매일칭중후복강주사，련속3천。정상사양지1월령，최후각조선취8지대서，삼가수미궁측시。결과분파비타간예조대서심조수하평태적잠복기교대조조명현연장，균존재현저성차이（t＝－3．542，P＝0．005）；천월유효구차수교대조조감소（t＝3．352，P＝0．005）。가용자오가보호후，여대조조비교，잠복기（t＝－1．313，P＝0．210）급천월유효구차수（t＝0．051，P＝0．128）균무현저성차이。탁필지간예조대서여대조조비교잠복기（t＝－1．920，P＝0．075）、천월유효구차수（t＝1．915，P＝0．076）；탁필지조여탁필지＋자오가조비교잠복기（t＝－0．597，P＝0．560）、천월유효구차수（t＝1．660，P＝0．119），균무현저성차이。결론미성숙뇌단기응용분파비타손해원기학습기억，자오가구유보호작용，단기응용중등제량적탁필지불회대미성숙뇌산생원기학습기억장애。
Objective To observe the influence of Phenobarbital ( PB) and Topiramate ( TPM) on long-term learning and memory of newly-born rats by short-term administration and the protection effect of Acanthopanax.Methods There were two batches of rats.Among the first batch, 30 3-day old healthy Sprague-Dawley ( SD) rats were randomly divided into 3 groups, control group, PB group and PB combined with Acanthopanax group.Among the second batch, 30 3-day old healthy Sprague-Dawley ( SD) rats were randomly divided into 3 groups with 10 rats in each, control group, TPM group and TPM combined with acanthopanx group.Hypertonic saline (10mL? kg-1? d-1 ) was given to the control group, PB group was injected with PB (62.5mg? kg-1? d-1), and PB (62.5mg? kg-1? d-1) and Acanthopanax (5.6g? kg-1? d-1 ) were injected to PB combined with Acanthopanax group.TMP was injected to TMP group with the dose of 62.5mg?kg-1? d-1 , and TMP combined with Acanthopanax group were given 62.5mg? kg-1? d-1 of TMP and 5.6g? kg-1? d-1 of Acanthopanax.The rats were injected after weighed for three continuous days.They were regularly fed till they were one month old.Finally 8 out of each group were selected to attend the game of Morris water maze.Results The incubation period for searching underwater platform in PB group was longer than the control group, and the difference was significant ( t=-3.542,P=0.005) .The times passing through the effective area reduced in PB group compared with the control group (t=3.352,P=0.005).However, after adding Acanthopanax, the incubation period (t=1.313,P=0.210) and the times passing through the effective area (t =0.051,P=0.128) had no significant difference from the control group.The incubation period for searching underwater platform in TPM group was significantly longer than the control group (t=-1.920,P=0.075), but the times passing through the effective area was less than the control group (t=1.915,P=0.076) .However, TPM group was not significantly different from TPM combined with Acanthopanax group in incubation period ( t =-0.597,P=0.560) and the times passing through the effective area (t =1.660,P=0.119).Conclusion Short-term use of PB in immature brain will damage long-term learning and memory, while Acanthopanax has protective effect.Short-term use of moderate dose of TPM in immature brain will not produce long-term learning and memory damage.