中国脑血管病杂志
中國腦血管病雜誌
중국뇌혈관병잡지
CHINESE JOURNAL OF CEREBROVASCULAR DISEASES
2015年
5期
245-249
,共5页
动脉粥样硬化%亲环素A%Krüppel样转录因子类%一氧化氮合酶%Krüppel样转录因子2
動脈粥樣硬化%親環素A%Krüppel樣轉錄因子類%一氧化氮閤酶%Krüppel樣轉錄因子2
동맥죽양경화%친배소A%Krüppel양전록인자류%일양화담합매%Krüppel양전록인자2
Atherosclerosis%Cyclophilin A%Krüppel-like factors%Nitric oxide synthase%Krüppel-like factor 2
目的:观察大鼠动脉粥样硬化病变过程中血管壁亲环素A( CyPA)、 Krüppel样转录因子2(KLF2)、内皮型一氧化氮合酶(eNOS)的表达变化。方法将雄性Wistar大鼠32只按数字表法随机分为对照组及动脉粥样硬化8、12、15周组,每组8只。给予动脉粥样硬化组高脂饲料喂养,并给予维生素D3注射液60万IU/kg一次性腹腔注射;给予对照组大鼠基础饲料喂养。在喂养第8、12、15周时处死大鼠,分离腹主动脉,行苏木素-伊红染色,免疫组化法检测动脉壁CyPA、KLF2、eNOS的表达水平。结果随着动脉粥样硬化病变进展,对照组以及动脉粥样硬化8、12、15周组大鼠分别呈现出血管正常结构、内皮细胞破坏、平滑肌细胞增生、粥样斑块形成以及钙化斑块形成等不同的特征。对照组与动脉粥样硬化8、12、15周组间血管壁CyPA、KLF2、eNOS的表达水平差异均有统计学意义( CyPA:0.0037±0.0018、0.0276±0.0090、0.0526±0.0129、0.1080±0.0388;KLF2:0.0932±0.0331、0.0415±0.0076、0.0207±0.0059、0.0014±0.0003;eNOS:0.0358±0.0185、0.0148±0.0080、0.0049±0.0025、0.0037±0.0026;F值分别为36.395、42.108、21.255,均P<0.05),且组间CyPA的表达呈增高趋势,组间两两比较差异有统计学意义(均P<0.05);KLF2表达水平呈进行性下降趋势,组间两两比较差异有统计学意义(均P<0.05);eNOS表达动脉粥样硬化12周组与15周组差异无统计学意义,其他组间差异均有统计学意义(均P<0.05)。结论伴随动脉粥样硬化病变进展,CyPA的表达进行性增高,KLF2、eNOS表达进行性下降。 CyPA可能是致动脉粥样硬化病变的途径之一。
目的:觀察大鼠動脈粥樣硬化病變過程中血管壁親環素A( CyPA)、 Krüppel樣轉錄因子2(KLF2)、內皮型一氧化氮閤酶(eNOS)的錶達變化。方法將雄性Wistar大鼠32隻按數字錶法隨機分為對照組及動脈粥樣硬化8、12、15週組,每組8隻。給予動脈粥樣硬化組高脂飼料餵養,併給予維生素D3註射液60萬IU/kg一次性腹腔註射;給予對照組大鼠基礎飼料餵養。在餵養第8、12、15週時處死大鼠,分離腹主動脈,行囌木素-伊紅染色,免疫組化法檢測動脈壁CyPA、KLF2、eNOS的錶達水平。結果隨著動脈粥樣硬化病變進展,對照組以及動脈粥樣硬化8、12、15週組大鼠分彆呈現齣血管正常結構、內皮細胞破壞、平滑肌細胞增生、粥樣斑塊形成以及鈣化斑塊形成等不同的特徵。對照組與動脈粥樣硬化8、12、15週組間血管壁CyPA、KLF2、eNOS的錶達水平差異均有統計學意義( CyPA:0.0037±0.0018、0.0276±0.0090、0.0526±0.0129、0.1080±0.0388;KLF2:0.0932±0.0331、0.0415±0.0076、0.0207±0.0059、0.0014±0.0003;eNOS:0.0358±0.0185、0.0148±0.0080、0.0049±0.0025、0.0037±0.0026;F值分彆為36.395、42.108、21.255,均P<0.05),且組間CyPA的錶達呈增高趨勢,組間兩兩比較差異有統計學意義(均P<0.05);KLF2錶達水平呈進行性下降趨勢,組間兩兩比較差異有統計學意義(均P<0.05);eNOS錶達動脈粥樣硬化12週組與15週組差異無統計學意義,其他組間差異均有統計學意義(均P<0.05)。結論伴隨動脈粥樣硬化病變進展,CyPA的錶達進行性增高,KLF2、eNOS錶達進行性下降。 CyPA可能是緻動脈粥樣硬化病變的途徑之一。
목적:관찰대서동맥죽양경화병변과정중혈관벽친배소A( CyPA)、 Krüppel양전록인자2(KLF2)、내피형일양화담합매(eNOS)적표체변화。방법장웅성Wistar대서32지안수자표법수궤분위대조조급동맥죽양경화8、12、15주조,매조8지。급여동맥죽양경화조고지사료위양,병급여유생소D3주사액60만IU/kg일차성복강주사;급여대조조대서기출사료위양。재위양제8、12、15주시처사대서,분리복주동맥,행소목소-이홍염색,면역조화법검측동맥벽CyPA、KLF2、eNOS적표체수평。결과수착동맥죽양경화병변진전,대조조이급동맥죽양경화8、12、15주조대서분별정현출혈관정상결구、내피세포파배、평활기세포증생、죽양반괴형성이급개화반괴형성등불동적특정。대조조여동맥죽양경화8、12、15주조간혈관벽CyPA、KLF2、eNOS적표체수평차이균유통계학의의( CyPA:0.0037±0.0018、0.0276±0.0090、0.0526±0.0129、0.1080±0.0388;KLF2:0.0932±0.0331、0.0415±0.0076、0.0207±0.0059、0.0014±0.0003;eNOS:0.0358±0.0185、0.0148±0.0080、0.0049±0.0025、0.0037±0.0026;F치분별위36.395、42.108、21.255,균P<0.05),차조간CyPA적표체정증고추세,조간량량비교차이유통계학의의(균P<0.05);KLF2표체수평정진행성하강추세,조간량량비교차이유통계학의의(균P<0.05);eNOS표체동맥죽양경화12주조여15주조차이무통계학의의,기타조간차이균유통계학의의(균P<0.05)。결론반수동맥죽양경화병변진전,CyPA적표체진행성증고,KLF2、eNOS표체진행성하강。 CyPA가능시치동맥죽양경화병변적도경지일。
Objective To observe the expression changes of cyclophilin A ( CyPA ) , Krüppel-like factor 2 (KLF2),and endothelial nitric oxide synthase (eNOS) in the pathological process of atherosclerosis in rats. Methods Thirty-two male Wistar rats were randomly divided into four groups:a control group and atherosclerosis 8,12,and 15 week groups (n=8 in each group). The atherosclerosis groups were fed with high-fat diet,and they were given vitamin D3 600 000 IU for a single intraperitoneal injection,and the rats in the control group were fed with the basal feed. The rats were sacrificed at 8,12,and 15 weeks. Their abdominal aortas were separated and stained with hematoxylin-eosin staining. Immunohistochemical method was used to detect the expression levels of CyPA,KLF2,and eNOS on arterial walls. Results With the progression of atherosclerosis lesion,the rats in the control group,and atherosclerosis 8,12,and 15 week groups showed different characteristics, including normal vessel structure, endothelial cell damage, smooth muscle cell proliferation,formation of atherosclerotic plaque,and formation of calcified plaque. There were significantly differences in the expression levels of CyPA,KLF2,eNOS on vessel walls among the control group,atherosclerosis 8,12,and 15 week groups (CyPA:0. 0037 ± 0. 0018,0. 0276 ± 0. 0090,0. 0526 ± 0. 0129,and 0. 1080 ± 0. 0388;KLF2:0. 0932 ± 0. 0331,0. 0415 ± 0. 0076,0. 0207 ± 0. 0059, and 0. 0014 ± 0. 0003;eNOS:0. 0358 ± 0. 0185,0. 0148 ± 0. 0080,0. 0049 ± 0. 0025,0. 0037 ± 0. 0026;F=36. 395,42. 108,and 21. 255,respectively,all P<0. 05),and the CyPA expression showed an increasing trend among groups, and there were significant differences between the pairwise comparisons (all P<0. 05). The expression level of KLF2 showed a progressive declining trend,and there were significant differences between pairwise comparisons (all P <0. 05);there was significant difference in eNOS expression between the atherosclerosis 12-week group and the atherosclerosis 15-week group, and there were significant differences among other groups (all P<0. 05). Conclusion With the progression of atherosclerotic lesions, the expression of CyPA increases progressively, and the expression levels of KLF2 and eNOS decrease progressively. This may be one of the approaches of CyPA caused atherosclerotic lesion.