中华老年病研究电子杂志
中華老年病研究電子雜誌
중화노년병연구전자잡지
Chinese Journal of Senile Diseases Research (Electronic Edition)
2014年
1期
19-23
,共5页
刘正霞%蔡诗昆%周萍%刘莹%鲁翔
劉正霞%蔡詩昆%週萍%劉瑩%魯翔
류정하%채시곤%주평%류형%로상
雌激素类%血管紧张素Ⅱ%高血压%心肌病 ,肥厚性%小鼠
雌激素類%血管緊張素Ⅱ%高血壓%心肌病 ,肥厚性%小鼠
자격소류%혈관긴장소Ⅱ%고혈압%심기병 ,비후성%소서
Estrogen s%Angiotensin Ⅱ%Hypertension%Cardiomyopathy,hypertrophic%Mice
目的:探讨雌激素对血管紧张素Ⅱ(AngⅡ)诱导的高血压心肌肥厚小鼠T淋巴细胞免疫调节作用的影响。方法取8~10周龄雌性C57BL/6小鼠31只,采用随机数字表法分为4组:假手术组(Sham组,8只),假手术+AngⅡ处理组(Sham+AngⅡ组,7只),卵巢切除组(Ovx组,8只)、卵巢切除+AngⅡ处理组(Ovx+AngⅡ组,8只)。AngⅡ给予方法:采用微量缓释泵植入小鼠颈背部皮下进行灌注(1000 ng?kg-1?d-1,持续4周),测定给药前及给药后第2,3,4,5周动脉血压,给药结束后行经胸廓超声心动图检测射血分数(EF )、左心室缩短分数(FS )、左心室舒张/收缩末期室间隔厚度(IVSd/IVSs )、左心室舒张/收缩末期容积(LV VOLd/LV VOLs )、左心室舒张/收缩末期后壁厚度(LVPWTd/LVPWTs )。流式细胞仪检测外周血CD3+,CD4+,CD8+的百分数结果。多组间血压比较采用方差分析,两组间心脏超声检测指标及T淋巴细胞亚群的比较采用 t检验。结果建模后各时点,Sham+AngⅡ组收缩压较Sham组显著升高、Ovx+AngⅡ组收缩压较Ovx组显著升高,差异均有统计学意义( F=15.23,P<0.01)。给予AngⅡ后,IVSs、IVSd、LVPWTs、LVPWTd均显著增高,差异均有统计学意义( t=3.81、7.74、3.87、7.73,8.64、6.01、4.93、8.59;P<0.05或0.01);与Sham+AngⅡ组比较,Ovx+AngⅡ组IVSs、IVSd、LVPWTs均显著增高,差异有统计学意义( t=2.00、2.64、1.71,均 P<0.01)。Sham+AngⅡ组及Ovx+AngⅡ组较之相对应的Sham组及Ovx组,CD3+比例升高、CD4+比例降低、CD8+比例升高、CD4+/CD8+比例降低,差异均有统计学意义( t=6.24、-4.85、7.70、-10.62,2.13、-11.04、11.11、-24.24;P<0.05);而且与Sham+AngⅡ组比较,Ovx+AngⅡ组总T细胞比例降低、CD4+比例升高、CD8+比例降低、CD4+/CD8+比例升高,差异均有统计学意义( t=-2.78、3.37、-3.42、3.16,均 P<0.05或0.01)。结论雌激素对于高血压心肌肥厚过程中的T淋巴细胞免疫具有重要的调节作用。
目的:探討雌激素對血管緊張素Ⅱ(AngⅡ)誘導的高血壓心肌肥厚小鼠T淋巴細胞免疫調節作用的影響。方法取8~10週齡雌性C57BL/6小鼠31隻,採用隨機數字錶法分為4組:假手術組(Sham組,8隻),假手術+AngⅡ處理組(Sham+AngⅡ組,7隻),卵巢切除組(Ovx組,8隻)、卵巢切除+AngⅡ處理組(Ovx+AngⅡ組,8隻)。AngⅡ給予方法:採用微量緩釋泵植入小鼠頸揹部皮下進行灌註(1000 ng?kg-1?d-1,持續4週),測定給藥前及給藥後第2,3,4,5週動脈血壓,給藥結束後行經胸廓超聲心動圖檢測射血分數(EF )、左心室縮短分數(FS )、左心室舒張/收縮末期室間隔厚度(IVSd/IVSs )、左心室舒張/收縮末期容積(LV VOLd/LV VOLs )、左心室舒張/收縮末期後壁厚度(LVPWTd/LVPWTs )。流式細胞儀檢測外週血CD3+,CD4+,CD8+的百分數結果。多組間血壓比較採用方差分析,兩組間心髒超聲檢測指標及T淋巴細胞亞群的比較採用 t檢驗。結果建模後各時點,Sham+AngⅡ組收縮壓較Sham組顯著升高、Ovx+AngⅡ組收縮壓較Ovx組顯著升高,差異均有統計學意義( F=15.23,P<0.01)。給予AngⅡ後,IVSs、IVSd、LVPWTs、LVPWTd均顯著增高,差異均有統計學意義( t=3.81、7.74、3.87、7.73,8.64、6.01、4.93、8.59;P<0.05或0.01);與Sham+AngⅡ組比較,Ovx+AngⅡ組IVSs、IVSd、LVPWTs均顯著增高,差異有統計學意義( t=2.00、2.64、1.71,均 P<0.01)。Sham+AngⅡ組及Ovx+AngⅡ組較之相對應的Sham組及Ovx組,CD3+比例升高、CD4+比例降低、CD8+比例升高、CD4+/CD8+比例降低,差異均有統計學意義( t=6.24、-4.85、7.70、-10.62,2.13、-11.04、11.11、-24.24;P<0.05);而且與Sham+AngⅡ組比較,Ovx+AngⅡ組總T細胞比例降低、CD4+比例升高、CD8+比例降低、CD4+/CD8+比例升高,差異均有統計學意義( t=-2.78、3.37、-3.42、3.16,均 P<0.05或0.01)。結論雌激素對于高血壓心肌肥厚過程中的T淋巴細胞免疫具有重要的調節作用。
목적:탐토자격소대혈관긴장소Ⅱ(AngⅡ)유도적고혈압심기비후소서T림파세포면역조절작용적영향。방법취8~10주령자성C57BL/6소서31지,채용수궤수자표법분위4조:가수술조(Sham조,8지),가수술+AngⅡ처리조(Sham+AngⅡ조,7지),란소절제조(Ovx조,8지)、란소절제+AngⅡ처리조(Ovx+AngⅡ조,8지)。AngⅡ급여방법:채용미량완석빙식입소서경배부피하진행관주(1000 ng?kg-1?d-1,지속4주),측정급약전급급약후제2,3,4,5주동맥혈압,급약결속후행경흉곽초성심동도검측사혈분수(EF )、좌심실축단분수(FS )、좌심실서장/수축말기실간격후도(IVSd/IVSs )、좌심실서장/수축말기용적(LV VOLd/LV VOLs )、좌심실서장/수축말기후벽후도(LVPWTd/LVPWTs )。류식세포의검측외주혈CD3+,CD4+,CD8+적백분수결과。다조간혈압비교채용방차분석,량조간심장초성검측지표급T림파세포아군적비교채용 t검험。결과건모후각시점,Sham+AngⅡ조수축압교Sham조현저승고、Ovx+AngⅡ조수축압교Ovx조현저승고,차이균유통계학의의( F=15.23,P<0.01)。급여AngⅡ후,IVSs、IVSd、LVPWTs、LVPWTd균현저증고,차이균유통계학의의( t=3.81、7.74、3.87、7.73,8.64、6.01、4.93、8.59;P<0.05혹0.01);여Sham+AngⅡ조비교,Ovx+AngⅡ조IVSs、IVSd、LVPWTs균현저증고,차이유통계학의의( t=2.00、2.64、1.71,균 P<0.01)。Sham+AngⅡ조급Ovx+AngⅡ조교지상대응적Sham조급Ovx조,CD3+비례승고、CD4+비례강저、CD8+비례승고、CD4+/CD8+비례강저,차이균유통계학의의( t=6.24、-4.85、7.70、-10.62,2.13、-11.04、11.11、-24.24;P<0.05);이차여Sham+AngⅡ조비교,Ovx+AngⅡ조총T세포비례강저、CD4+비례승고、CD8+비례강저、CD4+/CD8+비례승고,차이균유통계학의의( t=-2.78、3.37、-3.42、3.16,균 P<0.05혹0.01)。결론자격소대우고혈압심기비후과정중적T림파세포면역구유중요적조절작용。
Objective To investigate the effect of the estrogenfor on T lymphocyte immunoregulation in mice with hypertrophic cardiomyopathy caused by AngⅡ .Methods Thirty one female C57BL/6 mice between 8~10 weeks were randomly divided into 4 groups:Sham (n=8) ,Sham+AngⅡ (n=7) ,Ovx (n=8) ,Ovx+AngⅡ (n=8) .Osmotic mini pumps containing AngⅡ (1000 ng?kg-1?d-1 ) were implanted subcutaneously in experimental mice .Thirty days after mini pump implantation ,blood pressure and heart rate were measured noninvasively in conscious animals by the tail cuff method .Transthoracic echocardiography was performed under light sedation with 1 .0% isoflurane .Ejection fraction(EF) , fractional shortening (FS ) , diastolic/systolic ventricular septal (IVSd/IVSs ) and posterior wall thickness (LVPWTd/LVPWTs) ,left ventricular diastolic and systolic volumes (LV VOLd/LV VOLs) were measured before and at the second , third ,fourth ,fifth week after the treatment .The immunologic markers of T cells CD3+ ,CD4+ ,CD8+ were analyzed by multicolor flow cytometry .Results The hypertension and hypertrophic cardiomyopathy model was set up in the mice by infusion of AngⅡ (1000 ng/kg per day) .Each time point after modeling ,Sham+AngⅡ group showed significantly higher systolic blood pressure than in the sham group ,Ovx+AngⅡ group increased in systolic blood pressure compared with Ovx group .There were significant difference ( F=15.23 ,P<0.01 ) .Compared with the Sham group ,Sham+AngⅡ group indicated significantly increased IVSs ,IVSd ,LVPWs ,LVPWd ( t=3.81、7.74、3.87、7.73 ,P<0 .01 );Compared with Ovx ,Ovx+AngⅡ group showed significantly increased IVSs ,IVSd ,LVPWs ,LVPWd and significantly reduced LV VOLs , LV VOLd ( t=8.64、6.01、4.93、8.59、-2.16、-2.58 ,P<0 .05 or 0 .01 ) .Compared to Sham and Ovx group ,sham+AngⅡ and Ovx+AngⅡ group had the higher percentage of T cell ratios with reduced CD4+ and increased CD8+ cells ( t=6.24、-4.85、7.70、-10.62、2.13、-11.04、11.11、-24.24 ,P<0 .05 or 0.01);But compared with the sham+Ang group ,Ovx+Ang group percentage of total T cells decrease with higher CD4+ ratio and lower CD8+ proportions ( t= -2.78、3.37、-3.42、3.16 ,P<0 .05 or 0 .01) .Conclusion Our studies shows that estrogen plays an important role in T cellimmunoregulationfor in AngⅡ induced hypertrophic cardiomyopathy .