CAJ | 학술논문

为了探讨干旱胁迫对沙棘叶片黄酮类化合物代谢的影响，以肋果沙棘试管苗为材料，研究了不同浓度 PEG‐6000（0％，10％，20％，30％）对叶片类黄酮积累及其合成过程中相关酶PAL、C4H和4CL活性的影响．结果显示：（1）随干旱胁迫程度的增强，总黄酮、槲皮素、山奈酚和杨梅素含量均表现为先下降后上升的趋势，20％ PEG‐6000胁迫下其含量最低，比对照分别下降了20.36％，12.84％，47.81％，63.22％；在不同浓度 PEG‐6000胁迫下异鼠李素含量与对照相比均有升高，10％ PEG‐6000胁迫下其含量最高，比对照升高了79.99％．（2）干旱胁迫显著影响肋果沙棘试管苗叶片PAL、C4H和4CL的活性（P＜0.05）．随着胁迫程度的增加， PAL活性显著升高；C4H和4CL活性呈先升高后降低的趋势，在20％ PEG‐6000胁迫下达到最高．（3）杨梅素、槲皮素、山奈酚及总黄酮含量均与PAL、C4H和4C L活性负相关．尽管干旱胁迫促进了相关酶的活性，但肋果沙棘试管苗叶片中总黄酮含量并没有显著变化．
위료탐토간한협박대사극협편황동류화합물대사적영향，이륵과사극시관묘위재료，연구료불동농도 PEG‐6000（0％，10％，20％，30％）대협편류황동적루급기합성과정중상관매PAL、C4H화4CL활성적영향．결과현시：（1）수간한협박정도적증강，총황동、곡피소、산내분화양매소함량균표현위선하강후상승적추세，20％ PEG‐6000협박하기함량최저，비대조분별하강료20.36％，12.84％，47.81％，63.22％；재불동농도 PEG‐6000협박하이서리소함량여대조상비균유승고，10％ PEG‐6000협박하기함량최고，비대조승고료79.99％．（2）간한협박현저영향륵과사극시관묘협편PAL、C4H화4CL적활성（P＜0.05）．수착협박정도적증가， PAL활성현저승고；C4H화4CL활성정선승고후강저적추세，재20％ PEG‐6000협박하체도최고．（3）양매소、곡피소、산내분급총황동함량균여PAL、C4H화4C L활성부상관．진관간한협박촉진료상관매적활성，단륵과사극시관묘협편중총황동함량병몰유현저변화．
Taking test‐tube plantlets of Hippophae neurocarpa as materials ,the current study is carried out to investigate the effect of drought stress on the flavonoids accumulation and activities of Enzymes . There are four drought levels :the control(without adding PEG‐6000) ,10% PEG‐6000 ,20% PEG‐6000 , 30% PEG‐6000 . The result show that : (1 )With the serious of drought stress , the contents of quercetin , kaempferol , myricetin and total flavonoids contents decreased at first , and then increased , which reachs the lowest value under 20% PEG‐6000 , and the values are 20.36% ,12.84% ,47.81% , 63.22% lower than that of control , respectively . The content of isorhamnetin is increased under PEG‐6000 stress compared with control , and it reachs the highest value under 10% PEG‐6000 , and the value is 79.99% higher than that of control ;(2) A significant difference for PAL ,C4H and 4CL is found in test‐tube plantlets leaves of H.neurocarpa under the drought stress(P<0.05) . With the serious of drought stress ,the activity of PAL is significantly increased .The activity of C4H and 4CL increased at first ,and then decreased , which reachs the highest value under 20% PEG‐6000 ; (3) Linear regression analysis shows that the contents of kaempferol ,myricetin ,the total flavonoids and the activities of PAL ,C4H , 4CL present negative correlation . Although the related enzyme activities are increased by drought stress , the contents of total flavonoids in test‐tube plantlets leaves of H.neurocarpa is not significantly changed .