中国妇幼健康研究
中國婦幼健康研究
중국부유건강연구
CHINESE JOURNAL OF MATERNAL AND CHILD HEALTH RESEARCH
2015年
2期
248-250,314
,共4页
丁晓颖%黄鹰%王晓波%高青云%储莉鸣
丁曉穎%黃鷹%王曉波%高青雲%儲莉鳴
정효영%황응%왕효파%고청운%저리명
核因子-κB%环氧合酶-2%醛糖还原酶1%临产%绒毛膜
覈因子-κB%環氧閤酶-2%醛糖還原酶1%臨產%絨毛膜
핵인자-κB%배양합매-2%철당환원매1%임산%융모막
nuclear factor kappa B(NF-κB)%cyclooxygenase-2(COX-2)%aldo-ketoreductase B-1(AKR1B1)%labor%chorion
目的：分析足月妊娠妇女临产前后绒毛膜中核因子－κB（ NF－κB）的激活与前列腺素F（ PGF）合成调节酶的蛋白表达变化及其相关性。方法收集足月妊娠临产及未临产妇女各15例的绒毛膜组织，采用Western Blot方法检测其中NF－κB的激活水平以及环氧合酶（ COX）－2、醛糖还原酶（ AKR1B）1的蛋白水平，并分析NF－κB的激活水平与COX－2、AKR1B1表达的相关性。结果①临产组绒毛膜中COX－2、AKR1B1的蛋白水平均高于未临产组，差异均有极显著性（ F值分别为7．737和8．772，均P＜0．01）；NF－κB的激活水平也高于未临产组，差异有显著性（F＝38．563，P＜0．05）；②临产前后绒毛膜中NF－κB的激活水平与COX－2、AKR1B1的表达水平均呈显著正相关（r值分别为0．243和0．168，均P＜0．05）。结论临产后绒毛膜中激活的NF－κB和PGF合成调节酶的表达水平增高，且NF－κB的激活可以上调PGF合成调节酶表达的过程，导致PGF合成增加，这可能是NF－κB参与足月妊娠分娩启动的机制之一。
目的：分析足月妊娠婦女臨產前後絨毛膜中覈因子－κB（ NF－κB）的激活與前列腺素F（ PGF）閤成調節酶的蛋白錶達變化及其相關性。方法收集足月妊娠臨產及未臨產婦女各15例的絨毛膜組織，採用Western Blot方法檢測其中NF－κB的激活水平以及環氧閤酶（ COX）－2、醛糖還原酶（ AKR1B）1的蛋白水平，併分析NF－κB的激活水平與COX－2、AKR1B1錶達的相關性。結果①臨產組絨毛膜中COX－2、AKR1B1的蛋白水平均高于未臨產組，差異均有極顯著性（ F值分彆為7．737和8．772，均P＜0．01）；NF－κB的激活水平也高于未臨產組，差異有顯著性（F＝38．563，P＜0．05）；②臨產前後絨毛膜中NF－κB的激活水平與COX－2、AKR1B1的錶達水平均呈顯著正相關（r值分彆為0．243和0．168，均P＜0．05）。結論臨產後絨毛膜中激活的NF－κB和PGF閤成調節酶的錶達水平增高，且NF－κB的激活可以上調PGF閤成調節酶錶達的過程，導緻PGF閤成增加，這可能是NF－κB參與足月妊娠分娩啟動的機製之一。
목적：분석족월임신부녀임산전후융모막중핵인자－κB（ NF－κB）적격활여전렬선소F（ PGF）합성조절매적단백표체변화급기상관성。방법수집족월임신임산급미임산부녀각15례적융모막조직，채용Western Blot방법검측기중NF－κB적격활수평이급배양합매（ COX）－2、철당환원매（ AKR1B）1적단백수평，병분석NF－κB적격활수평여COX－2、AKR1B1표체적상관성。결과①임산조융모막중COX－2、AKR1B1적단백수평균고우미임산조，차이균유겁현저성（ F치분별위7．737화8．772，균P＜0．01）；NF－κB적격활수평야고우미임산조，차이유현저성（F＝38．563，P＜0．05）；②임산전후융모막중NF－κB적격활수평여COX－2、AKR1B1적표체수평균정현저정상관（r치분별위0．243화0．168，균P＜0．05）。결론임산후융모막중격활적NF－κB화PGF합성조절매적표체수평증고，차NF－κB적격활가이상조PGF합성조절매표체적과정，도치PGF합성증가，저가능시NF－κB삼여족월임신분면계동적궤제지일。
Objective To study the changes and relationship between activation of NF-κB and the expression of PGF synthetase in chorion before and after labor in term pregnancy.Methods Fifteen cases of term labor ( TL) and 15 cases of term non-labor ( TNL) were selected as the objects of study.Western Blot test was used to observe the expression of activated NF-κB, COX-2 and AKR1B1 in their chorion, and the correlation between them was analyzed.Results Compared with the TNL group, the levels of COX-2 and AKR1B1 increased in TL group, there were significant differences between two groups (F value was 7.737 and 8.772, respectively, both P<0.01), and the level of activated NF-κB was also higher in TL group (F=38.563,P<0.05).Both the expressions of COX-2 and AKR1B1 were positively correlated with the activated NF-κB in chorion before and after labor (r value was 0.243 and 0.168, respectively, both P<0.05). Conclusion The levels of NF-κB, COX-2 and AKR1B1 protein increase in human chorion after labor, and the activated NF-κB can up-regulate the expression of PGF synthetase and lead to increased synthesis of PGF.It may be the mechanism of NF-κB participating in initiation of labor.
