CAJ | 학술논문

目的：分析陕西地区高危型人乳头瘤病毒（ HR－HPV）阳性，但宫颈细胞学正常和宫颈癌患者HPV病毒谱。方法利用PCR基因扩增方法，检测宫颈脱落细胞中8种HR－HPV DNA（包括HPV16、18、31、33、35、45、52、58等）并行细胞病理学检测。对1005例HR－HPV阳性但宫颈细胞学正常标本及160例经组织病理学证实宫颈癌标本进行HPV感染谱对比分析。结果正常细胞学组与宫颈癌组的HR－HPV型别分布除HPV35、45外，其余型别HPV16、18、31、33、52、58等差异均有统计学意义（χ2值分别为465．147、110．288、9．261、4．496、30．155、46．559，均P＜0．05）；正常细胞学组中HPV多为单一感染，而宫颈癌组中多重HPV感染较为常见。单一感染均以HPV16最为常见，在正常细胞学组和宫颈癌组分别占34．9％和37．6％。双重感染在正常细胞学组以HPV16合并HPV52最为常见（占2．9％）；而宫颈癌组则以HPV16合并HPV18最为常见（占20．8％）。结论正常细胞学组HPV感染谱与宫颈癌组中相同HPV型别分布的差异均有助于理解HPV致癌机理以及预防性HPV疫苗的设计。
목적：분석협서지구고위형인유두류병독（ HR－HPV）양성，단궁경세포학정상화궁경암환자HPV병독보。방법이용PCR기인확증방법，검측궁경탈락세포중8충HR－HPV DNA（포괄HPV16、18、31、33、35、45、52、58등）병행세포병이학검측。대1005례HR－HPV양성단궁경세포학정상표본급160례경조직병이학증실궁경암표본진행HPV감염보대비분석。결과정상세포학조여궁경암조적HR－HPV형별분포제HPV35、45외，기여형별HPV16、18、31、33、52、58등차이균유통계학의의（χ2치분별위465．147、110．288、9．261、4．496、30．155、46．559，균P＜0．05）；정상세포학조중HPV다위단일감염，이궁경암조중다중HPV감염교위상견。단일감염균이HPV16최위상견，재정상세포학조화궁경암조분별점34．9％화37．6％。쌍중감염재정상세포학조이HPV16합병HPV52최위상견（점2．9％）；이궁경암조칙이HPV16합병HPV18최위상견（점20．8％）。결론정상세포학조HPV감염보여궁경암조중상동HPV형별분포적차이균유조우리해HPV치암궤리이급예방성HPV역묘적설계。
Objective To analyze the spectrum of HPV in women with normal cytology and cervical cancer with high risk human papillomavirus (HR-HPV) in Shaanxi area.Methods HPV16, 18, 31, 33, 35, 45, 52, 58 DNA in cervical exfoliated cells were detected by PCR.Pap tests were performed for cytopathological analysis.Infection spectrum was analyzed and compared between 1 005 cases with positive HR-HPV and normal cytology and 160 cases of cervical cancer.Results The distribution of HPV16, 18, 31, 33, 52 and 58 was significantly different between normal cytology group and cervical cancer group except for HPV35 and 45 (χ2 value was 465.147, 110.288, 9.261, 4.496, 30.155 and 46.559, respectively, all P<0.05).Infection with single HPV type was more common in the normal cytology group, while infection with multiple HPV types were frequently found in the cervical cancer group.HPV16 was the most prevalent genotype detected in two groups, and the infection rate of it was 34.9% and 37.6% respectively in the normal cytology group and cervical cancer group.Considering multiple infection, HPV16 and 52 were more frequently found in the normal cytology group (2.9%), while in the cervical cancer group the most prevalent multiple infection pattern were HPV16 and 18 (20.8%).Conclusion The difference in the distribution of HPV types found both in normal cytology group and cervical cancer group is helpful for understanding HPV carcinogenic mechanism and designing of prophylactic vaccine.