CAJ | 학술논문

通过对磷脂酶A1( Lecitase Ultra)酶解磷脂酰胆碱( PC)过程进行分析,证实了Sn-2-溶血磷脂酰胆碱( Sn-2-LPC)存在酰基转移现象。依据前人对单甘酯自动酰基转移机理的研究,推测了Sn-2-LPC在酸、碱条件下自动酰基转移的机理,并利用HPLC-RID确定了Sn-2-LPC可以酰基转移生成Sn-1-LPC,而Sn-1-LPC不存在酰基转移现象。同时,以硼酸为例,推测Al( OH)3、Zn( OH)2、B( OH)33种两性氢氧化物抑制Sn-2-LPC酰基转移的机理,确定了3种抑制剂的最小添加量分别为0.97、0.85、0.72 mg/mL。为合成酰基磷脂混合物,以及解释产生混合酰基磷脂的生物合成途径(溶血磷脂中间体)提供了理论基础和数据支撑。
통과대린지매A1( Lecitase Ultra)매해린지선담감( PC)과정진행분석,증실료Sn-2-용혈린지선담감( Sn-2-LPC)존재선기전이현상。의거전인대단감지자동선기전이궤리적연구,추측료Sn-2-LPC재산、감조건하자동선기전이적궤리,병이용HPLC-RID학정료Sn-2-LPC가이선기전이생성Sn-1-LPC,이Sn-1-LPC불존재선기전이현상。동시,이붕산위례,추측Al( OH)3、Zn( OH)2、B( OH)33충량성경양화물억제Sn-2-LPC선기전이적궤리,학정료3충억제제적최소첨가량분별위0.97、0.85、0.72 mg/mL。위합성선기린지혼합물,이급해석산생혼합선기린지적생물합성도경(용혈린지중간체)제공료이론기출화수거지탱。
Acyl migration phenomenon of Sn-2-lysophosphatidylcholine( Sn-2-LPC) was confirmed through hydrolysis process analysis of phosphatidylcholine by phospholipase A1 ( Lecitase Ultra ) . Based on previous studies on the automatical acyl migration mechanism of monoglyceride, the automatical acyl migration mechanism of Sn-2-LPC in the acid and alkali conditions was speculated. Acyl migration di-rectionalities of Sn-2-LPC and Sn-1-LPC were explored by HPLC-RID. It was found that acyl mi-gration of Sn-2-LPC to Sn-1-LPC happened, while acyl migration of Sn-1-LPC was not present. Meanwhile, the inhibition mechanism of acyl migration of Sn-2 -LPC through Al(OH)3, Zn(OH)2 and B( OH) 3 was speculated by boric acid. The minimum dosages of Al( OH) 3 , Zn( OH) 2 and B( OH) 3 were determined as 0. 97, 0. 85 mg/mL and 0. 72 mg/mL. A theoretical basis and data support for the synthesis of mixed acyl phospholipids and the explanation of biosynthetic pathway ( lysophospholipid inter-mediate) of mixed acyl phospholipids were provided.