CAJ | 학술논문

目的：探讨比格犬胃窦黏膜组织学与超微结构的增龄变化特点。方法选取健康比格犬（犬龄1.5～11.0岁，体重14～20 kg ）19只。按年龄分组：≥1.5岁且≤6岁8只（中青年组），＞6岁且≤9岁5只（低龄老年组），＞9岁且≤11岁6只（高龄老年组）。光镜下观察对比各组犬胃窦黏膜上皮细胞形态学变化、胃窦黏膜炎症程度、肠上皮化生和异型增生情况、固有膜厚度及黏液腺体实质比的变化；透射电镜下观察对比各组犬胃窦黏膜上皮细胞形态、细胞间连接、幽门腺细胞黏原颗粒面积分数的变化。3组间胃黏膜炎症指数、胃窦黏膜固有膜厚度及黏液腺体实质比的比较采用单因素方差分析。结果光镜观察结果：各组犬胃窦黏膜上皮细胞未见明显形态学变化；胃窦黏膜内均未见肠上皮化生、异型增生，且无炎症或炎症表现轻微，各组间炎症指数无明显差异（ F＝0.023，P＞0.05）；中青年组、低龄老年组及高龄老年组胃窦黏膜固有膜厚度分别为（1016.7±203.2）、（727.5±64.3）、（931.7±92.4）μm ，差异有统计学意义（ F＝5.952，P＜0.05），其中高龄老年组黏膜内结缔组织增生明显；3组黏液腺体实质比分别为（45.3±6.4）％、（33.9±2.4）％、（34.5±6.3）％，差异有统计学意义（ F＝8.981，P＜0.01）。透射电镜观察结果：各组胃窦黏膜上皮细胞超微结构及细胞间紧密连接均未见明显改变，但细胞间中间连接及缝隙连接随增龄逐渐疏松，并可见空泡样缝隙；中青年组、低龄老年组及高龄老年组幽门腺细胞内黏原颗粒面积分数分别为（50.3±10.0）％、（44.4±3.9）％、（18.6±5.6）％，差异有统计学意义（ F＝15.501，P＜0.01）。结论健康比格犬胃窦黏膜组织学和超微结构随增龄出现了一系列退化现象。
목적：탐토비격견위두점막조직학여초미결구적증령변화특점。방법선취건강비격견（견령1.5～11.0세，체중14～20 kg ）19지。안년령분조：≥1.5세차≤6세8지（중청년조），＞6세차≤9세5지（저령노년조），＞9세차≤11세6지（고령노년조）。광경하관찰대비각조견위두점막상피세포형태학변화、위두점막염증정도、장상피화생화이형증생정황、고유막후도급점액선체실질비적변화；투사전경하관찰대비각조견위두점막상피세포형태、세포간련접、유문선세포점원과립면적분수적변화。3조간위점막염증지수、위두점막고유막후도급점액선체실질비적비교채용단인소방차분석。결과광경관찰결과：각조견위두점막상피세포미견명현형태학변화；위두점막내균미견장상피화생、이형증생，차무염증혹염증표현경미，각조간염증지수무명현차이（ F＝0.023，P＞0.05）；중청년조、저령노년조급고령노년조위두점막고유막후도분별위（1016.7±203.2）、（727.5±64.3）、（931.7±92.4）μm ，차이유통계학의의（ F＝5.952，P＜0.05），기중고령노년조점막내결체조직증생명현；3조점액선체실질비분별위（45.3±6.4）％、（33.9±2.4）％、（34.5±6.3）％，차이유통계학의의（ F＝8.981，P＜0.01）。투사전경관찰결과：각조위두점막상피세포초미결구급세포간긴밀련접균미견명현개변，단세포간중간련접급봉극련접수증령축점소송，병가견공포양봉극；중청년조、저령노년조급고령노년조유문선세포내점원과립면적분수분별위（50.3±10.0）％、（44.4±3.9）％、（18.6±5.6）％，차이유통계학의의（ F＝15.501，P＜0.01）。결론건강비격견위두점막조직학화초미결구수증령출현료일계렬퇴화현상。
Objective To observe the influence of aging on the degeneration morphological changes of antral mucosa .Methods Nineteen healthy Beagles were divided randomly into three groups:young group (≥1.5 years old and≤6 years old , n=8 ) ,junior elderly group (>6 years old and ≤9 years old , n=5 ) ,senior elderly group (>9 years old and ≤11 years old , n=6) .The antral mucosal histology was observed;semi quantitative analysis of inflammatory index were calculated;the thickness of lamina propria was measured and the mucosal gland density was calculated .The ultra structure of antral mucosal epithelial cells and pyloric glandular cells were studied and as well as cell junctions by electron microscopy .Results There was no intestinal metaplasia or dysplasia in any specimen .The morphology of epithelial cells and inflammation index of antral mucosal showed no significant changes among three groups .The thickness of lamina propria indicate no significant difference among young group (1016 .7 ± 203 .2 )μm ,junior elderly group (727 .5 ± 64 .3 )μm ,se-nior elderly group (931 .7 ± 92 .4)μm ( F=5.592 ,P<0 .05) .The proportion of mucosal glands decreased significantly with aging [(45 .3 ± 6 .4)% ,(33 .9 ± 2 .4)% ,(34 .5 ± 6 .3)% , F=8.981 ,P<0.01] .The ultra structures of epithelial cells and tight junctions had no significant changes;while middle junctions and gap junctions had gradually loosen with ag-ing .The area percentage of organelles which associated with mucus secretion ,decreased significantly with aging [(50 .3 ± 10 .0)% ,(44 .4 ± 3 .9)% ,(18 .6 ± 5 .6)% , F=15.501 , P<0 .01] .Conclusion The results suggest that the mor-phology of Beagles antral mucosa deteriorate significantly .