世界中西医结合杂志
世界中西醫結閤雜誌
세계중서의결합잡지
WORLD JOURNAL OF TRADITIONAL CHINESE AND WESTERN MEDICINE
2015年
4期
487-492
,共6页
马凌%孙红梅%龚小钢%和欣%高誉珊%张毅%胡京红%张淑静%盖聪%许红%郭振宇%蔡彦宁%张燕莉
馬凌%孫紅梅%龔小鋼%和訢%高譽珊%張毅%鬍京紅%張淑靜%蓋聰%許紅%郭振宇%蔡彥寧%張燕莉
마릉%손홍매%공소강%화흔%고예산%장의%호경홍%장숙정%개총%허홍%곽진우%채언저%장연리
帕金森病%牵正散%大补阴丸%MPP+%SH-SY5Y细胞%线粒体
帕金森病%牽正散%大補陰汍%MPP+%SH-SY5Y細胞%線粒體
파금삼병%견정산%대보음환%MPP+%SH-SY5Y세포%선립체
Parkinson's Disease%Qianzheng San%Dabuyin Wan%MPP+%SH-SY5Y%Mitochondria
目的：探讨牵正散合大补阴丸（ QZS&DBYW）对1-甲基-4-苯基吡啶（ MPP+）诱导的帕金森病（ PD）细胞模型线粒体的影响，为深入探讨中药防治PD提供实验依据。方法选择人多巴胺能神经母细胞瘤SH-SY5Y，用MPP+处理建立PD细胞模型，采用QZS&DBYW干预。CCK-8法检测各组细胞存活率，MitoTracker Red CMXRos（ MTR）对活细胞线粒体标记，共聚焦显微镜观察，ImageJ软件测量线粒体形态因子和长宽比。结果 MPP+处理后细胞存活率有明显下降， QZS&DBYW干预不会明显改变细胞存活率。相较于空白对照组，模型组细胞出现大量空泡，线粒体长宽比显著降低，线粒体碎片明显增多。单独施用低浓度QZS&DBYW后线粒体分支增加，而高浓度中药使线粒体更为短小。低浓度QZS&DBYW治疗组可增加PD细胞的线粒体长度。结论牵正散和大补阴丸两方合用可减少线粒体的碎裂，在一定程度上对PD细胞模型线粒体有一定保护作用。
目的：探討牽正散閤大補陰汍（ QZS&DBYW）對1-甲基-4-苯基吡啶（ MPP+）誘導的帕金森病（ PD）細胞模型線粒體的影響，為深入探討中藥防治PD提供實驗依據。方法選擇人多巴胺能神經母細胞瘤SH-SY5Y，用MPP+處理建立PD細胞模型，採用QZS&DBYW榦預。CCK-8法檢測各組細胞存活率，MitoTracker Red CMXRos（ MTR）對活細胞線粒體標記，共聚焦顯微鏡觀察，ImageJ軟件測量線粒體形態因子和長寬比。結果 MPP+處理後細胞存活率有明顯下降， QZS&DBYW榦預不會明顯改變細胞存活率。相較于空白對照組，模型組細胞齣現大量空泡，線粒體長寬比顯著降低，線粒體碎片明顯增多。單獨施用低濃度QZS&DBYW後線粒體分支增加，而高濃度中藥使線粒體更為短小。低濃度QZS&DBYW治療組可增加PD細胞的線粒體長度。結論牽正散和大補陰汍兩方閤用可減少線粒體的碎裂，在一定程度上對PD細胞模型線粒體有一定保護作用。
목적：탐토견정산합대보음환（ QZS&DBYW）대1-갑기-4-분기필정（ MPP+）유도적파금삼병（ PD）세포모형선립체적영향，위심입탐토중약방치PD제공실험의거。방법선택인다파알능신경모세포류SH-SY5Y，용MPP+처리건립PD세포모형，채용QZS&DBYW간예。CCK-8법검측각조세포존활솔，MitoTracker Red CMXRos（ MTR）대활세포선립체표기，공취초현미경관찰，ImageJ연건측량선립체형태인자화장관비。결과 MPP+처리후세포존활솔유명현하강， QZS&DBYW간예불회명현개변세포존활솔。상교우공백대조조，모형조세포출현대량공포，선립체장관비현저강저，선립체쇄편명현증다。단독시용저농도QZS&DBYW후선립체분지증가，이고농도중약사선립체경위단소。저농도QZS&DBYW치료조가증가PD세포적선립체장도。결론견정산화대보음환량방합용가감소선립체적쇄렬，재일정정도상대PD세포모형선립체유일정보호작용。
Objective To study the impacts of qianzheng san plus dabuyin wan( QZS&BDYW)on mitochondria in Parkinson's disease( PD)cell model induced by MPP+ so as to deeply explore the prevention and treatment of PD with Chinese medicine and provide the experimental evidence. Methods SH-SY5Y was selected and MPP+ treatment was adopted to set up PD cell model. QZS&BDYW was applied for inter-vention. CCK-8 method was used to detect the cell survival rate in each group. Mito Tracker Red CMXRos ( MTR)was adopted to label mitochondria of living cell. The confocal microscopy was used for observation. JmageJ software was used to measure morphologic factor and the ratio of length and width of mitochondria. Results After MPP+ treatment,the cell survival rate was reduced apparently. The intervention of QZS &BDYW did not obviously change the cell survival rate. Corresponding to the blank control group,a large a-mount of vacuoles appeared in cell of the model group,the length and width ratio of mitochondria was reduced significantly and mitochondria fragments were increased apparently. The mitochondria branches were in-creased after the use of QZS&BDYW at low concentration and mitochondria became much smaller after the use of Chinese medicine at high concentration. In the treatment group,QZS&BDYW at low concentration in-creased mitochondria length in PD cell. Conclusion Qianzheng san and dabuyin wan in combination reduce mitochondria fragmentation and bring a certain protective role for mitochondria in PD cell model.
