世界中西医结合杂志
世界中西醫結閤雜誌
세계중서의결합잡지
WORLD JOURNAL OF TRADITIONAL CHINESE AND WESTERN MEDICINE
2015年
4期
483-486
,共4页
陈超%陈慧彬%滕鸣健%窦永起
陳超%陳慧彬%滕鳴健%竇永起
진초%진혜빈%등명건%두영기
扶正散结方%Lewis肺癌%乏氧诱导因子-1α%环氧化酶-2%肿瘤微血管密度
扶正散結方%Lewis肺癌%乏氧誘導因子-1α%環氧化酶-2%腫瘤微血管密度
부정산결방%Lewis폐암%핍양유도인자-1α%배양화매-2%종류미혈관밀도
Fuzheng Sanjie Recipe%Lewis lung cancer%Hypoxia inducible factor-1α%cyclooxygen-ase-2%Tumor micro vessel density
目的：观察扶正散结汤拆方对Lewis肺癌小鼠移植瘤微血管密度的影响及其在缺氧诱导微血管生成通路的作用机制。方法 C57BL/6小鼠80只，随机分成空白组、模型组、顺铂组（ DDP组）、益气扶正组（扶正组）、化痰散结组（化痰组）、活血化瘀组（活血组）、清热解毒组（解毒组）和联合用药组，每组10只，除空白组外其余各组建立Lewis肺癌移植瘤模型后，分别给予相应药物干预，连续14 d，于第15天处死后剥离肿瘤组织，免疫组化SP法检测肿瘤组织中CD34、肿瘤组织中缺氧诱导因子1α（HIF-1α）、环氧化酶2（COX-2）表达。结果各用药组均能明显降低瘤组织MVD，以联用组最接近DDP组，其次为扶正组和活血组；与模型组比较，顺铂组及各中药组HIF-1α表达均降低（P﹤0.05，P﹤0.01），其中联用组、顺铂组及扶正组降低较明显。与模型组比较，各用药组COX-2表达量均呈下降趋势，以解毒组与联用组最低（ P﹤0.01），其次为活血组和化痰组，而DDP组与扶正组最接近于模型组，差异无统计学意义。结论扶正散结汤各拆方组分能抑制肿瘤血管新生，各抗癌组分在肿瘤缺氧通路及之外的其他通路上抑制肿瘤血管生成具有不同的作用特点和机制，而联合应用则可最大程度发挥抑制血管生成的作用。
目的：觀察扶正散結湯拆方對Lewis肺癌小鼠移植瘤微血管密度的影響及其在缺氧誘導微血管生成通路的作用機製。方法 C57BL/6小鼠80隻，隨機分成空白組、模型組、順鉑組（ DDP組）、益氣扶正組（扶正組）、化痰散結組（化痰組）、活血化瘀組（活血組）、清熱解毒組（解毒組）和聯閤用藥組，每組10隻，除空白組外其餘各組建立Lewis肺癌移植瘤模型後，分彆給予相應藥物榦預，連續14 d，于第15天處死後剝離腫瘤組織，免疫組化SP法檢測腫瘤組織中CD34、腫瘤組織中缺氧誘導因子1α（HIF-1α）、環氧化酶2（COX-2）錶達。結果各用藥組均能明顯降低瘤組織MVD，以聯用組最接近DDP組，其次為扶正組和活血組；與模型組比較，順鉑組及各中藥組HIF-1α錶達均降低（P﹤0.05，P﹤0.01），其中聯用組、順鉑組及扶正組降低較明顯。與模型組比較，各用藥組COX-2錶達量均呈下降趨勢，以解毒組與聯用組最低（ P﹤0.01），其次為活血組和化痰組，而DDP組與扶正組最接近于模型組，差異無統計學意義。結論扶正散結湯各拆方組分能抑製腫瘤血管新生，各抗癌組分在腫瘤缺氧通路及之外的其他通路上抑製腫瘤血管生成具有不同的作用特點和機製，而聯閤應用則可最大程度髮揮抑製血管生成的作用。
목적：관찰부정산결탕탁방대Lewis폐암소서이식류미혈관밀도적영향급기재결양유도미혈관생성통로적작용궤제。방법 C57BL/6소서80지，수궤분성공백조、모형조、순박조（ DDP조）、익기부정조（부정조）、화담산결조（화담조）、활혈화어조（활혈조）、청열해독조（해독조）화연합용약조，매조10지，제공백조외기여각조건립Lewis폐암이식류모형후，분별급여상응약물간예，련속14 d，우제15천처사후박리종류조직，면역조화SP법검측종류조직중CD34、종류조직중결양유도인자1α（HIF-1α）、배양화매2（COX-2）표체。결과각용약조균능명현강저류조직MVD，이련용조최접근DDP조，기차위부정조화활혈조；여모형조비교，순박조급각중약조HIF-1α표체균강저（P﹤0.05，P﹤0.01），기중련용조、순박조급부정조강저교명현。여모형조비교，각용약조COX-2표체량균정하강추세，이해독조여련용조최저（ P﹤0.01），기차위활혈조화화담조，이DDP조여부정조최접근우모형조，차이무통계학의의。결론부정산결탕각탁방조분능억제종류혈관신생，각항암조분재종류결양통로급지외적기타통로상억제종류혈관생성구유불동적작용특점화궤제，이연합응용칙가최대정도발휘억제혈관생성적작용。
Objective To observe the mechanism of Fuzheng Sanjie Recipe on Lewis lung cancer mice transplanted tumor microvessel density and hypoxia induced angiogenesis pathway. Methods Eighty C57BL/6 mice were randomly divided into Blank group,Model group,DDP group,Yiqifuzheng group,Hua-tansanjie group,Huoxuehuayu group,Qingrejiedu group and Combination group. In addition to the blank group,other groups to establish Lewis lung cancer xenograft model,and received drugs intervention for 14 days. On Day Fifteen,tumor tissue were stripped after animal was killed. Detected the expression of CD34, HIF-1α,COX-2 by streptavidin-perosidase immunohistochemical method in tumor tissue. Results The treatment groups significantly reduced the tumor microvessel density,combination group close to the DDP group,followed by Fuzheng group and Huoxue group. Compared with the model group,the expression of HIF-1αin DDP group and TCM groups decreased(P﹤0. 05,P﹤0. 01). DDP group,Combination group and Fuzheng group decreased significantly(there were no significant difference among the three groups),being followed by Huoxue group,Jiedu group and Huatan group where no difference as compared with prior the study was ob-served. The expression of COX-2 decreased in all the other groups decreased,when compared with the model group,with Jiedu group and Combination group being the lowest(P﹤0. 01),followed by Huoxue group and Huatan group,DDP group and Fuzheng group close to the model group. Conclusion Fuzheng Sanjie Recipe groups can inhibit tumor angiogenesis,the anticancer components in tumor hypoxia pathway and other path-ways on the inhibition of tumor angiogenesis with different characteristics and mechanisms,and the combined application can maximize the effects of inhibiting angiogenesis.
