CAJ | 학술논문

目的：采用变性梯度凝胶电泳聚合酶链反应（ PCR－DGGE）技术从微生物生态学的角度分析比较喂养不耐受（ FI）与健康早产儿肠道细菌群落结构的多样性及相似性。方法以2013年11月至2014年9月在第四军医大学附属唐都医院儿科新生儿病房诊断为FI的早产儿为FI组。选择与FI组胎龄、日龄、出生体重相匹配的非FI早产儿作为对照组。采集出现FI时和同时间段对照组的粪便标本，进行16SrDNAV3区扩增和变性梯度凝胶电泳（DGGE），从而分析比较两组间肠道菌群多样性指数及相似性。结果细菌多样性检测显示FI组的肠道菌群多样性指数香农－维纳指数（H）、丰度（S）、均衡度指数（E）和辛普森多样性指数（D）均低于对照组（均P＜0．05）；相似性矩阵图及聚类分析结果显示组内菌群相似性较组间高（P＜0．05）；PCA结果同聚类分析一致。结论肠道微生物群落多样性的改变及群落结构紊乱可能是引起早产儿FI的重要因素。
목적：채용변성제도응효전영취합매련반응（ PCR－DGGE）기술종미생물생태학적각도분석비교위양불내수（ FI）여건강조산인장도세균군락결구적다양성급상사성。방법이2013년11월지2014년9월재제사군의대학부속당도의원인과신생인병방진단위FI적조산인위FI조。선택여FI조태령、일령、출생체중상필배적비FI조산인작위대조조。채집출현FI시화동시간단대조조적분편표본，진행16SrDNAV3구확증화변성제도응효전영（DGGE），종이분석비교량조간장도균군다양성지수급상사성。결과세균다양성검측현시FI조적장도균군다양성지수향농－유납지수（H）、봉도（S）、균형도지수（E）화신보삼다양성지수（D）균저우대조조（균P＜0．05）；상사성구진도급취류분석결과현시조내균군상사성교조간고（P＜0．05）；PCA결과동취류분석일치。결론장도미생물군락다양성적개변급군락결구문란가능시인기조산인FI적중요인소。
Objective To observe and compare the diversity and similarity of intestinal bacterial community structure of preterm infants who had feeding intolerance ( FI ) and those who were healthy from the perspective of microbial ecology using polymerase chain reaction-denaturing gradient gel electrophoresis ( PCR-DGGE) technology.Methods The preterm infants diagnosed as FI during December 2013 to September 2014 in neonatal wards of pediatric department of Tangdu Hospital of the Fourth Military University were recruited in FI group. The infants without FI but matched in gestational age, days of age and birth weight during the same period were taken in control group.The stool samples were collected in FI group when FI occurred and in the control group at the same period for conducting 16SrDNA V3 region amplification and DGGE, and thus the diversity and similarity of intestinal bacterial community structure between two groups were analyzed and compared.Results The results of bacterial diversity showed that H, S, E and D of FI group were lower than those of the control group (all P <0.05).Similarity matrix and cluster analysis showed that the intra-group similarity of the flora was higher than inter-group similarity (P<0.05).Principal components analysis (PCA) was consistent with the cluster analysis.Conclusion The change of intestinal microbial community diversity and community structure disorder may be the important factor causing FI in preterm infants.