CAJ | 학술논문

以乳木果液油为原料,经甲酯化后得到低酸值的乳木果液油,通过转酯化把甘三酯转化为脂肪酸甲酯,通过分子蒸馏把脂肪酸甲酯和生物活性物质分为轻组分和重组分,重组分(生物活性物质)再经过溶剂结晶进一步提高生物活性物质含量。通过单因素实验确定较好的甲酯化工艺条件为：反应温度70℃,反应时间3 h,醇油质量比3∶10,浓硫酸添加量3%；在此条件下,乳木果液油的酸值(KOH)从56.1 mg/g降至2.1 mg/g,游离脂肪酸转化率达到96.3%。通过单因素实验确定的较佳分子蒸馏条件为：进料速率2 mL/min,刮膜转速250 r/min,蒸馏温度150℃,真空度0.1 Pa；在此条件下蒸馏,生物活性物质在重组分中的回收率达95.9%,生物活性物质含量可达68.8%。乳木果液油经过甲酯化、转酯化、分子蒸馏和结晶得到的产品中三萜醇和甾醇总含量可达69.80%,生物活性物质含量可达83.18%。
이유목과액유위원료,경갑지화후득도저산치적유목과액유,통과전지화파감삼지전화위지방산갑지,통과분자증류파지방산갑지화생물활성물질분위경조분화중조분,중조분(생물활성물질)재경과용제결정진일보제고생물활성물질함량。통과단인소실험학정교호적갑지화공예조건위：반응온도70℃,반응시간3 h,순유질량비3∶10,농류산첨가량3%；재차조건하,유목과액유적산치(KOH)종56.1 mg/g강지2.1 mg/g,유리지방산전화솔체도96.3%。통과단인소실험학정적교가분자증류조건위：진료속솔2 mL/min,괄막전속250 r/min,증류온도150℃,진공도0.1 Pa；재차조건하증류,생물활성물질재중조분중적회수솔체95.9%,생물활성물질함량가체68.8%。유목과액유경과갑지화、전지화、분자증류화결정득도적산품중삼첩순화치순총함량가체69.80%,생물활성물질함량가체83.18%。
With shea olein as raw material, low acid value shea olein was obtained by methyl esterification, then triglyceride was transformed into fatty acid methyl ester by transesterification. After the fatty acid methyl ester and bioactive compounds were separated into light fraction and heavy fraction by molecular dis-tillation, the heavy fraction ( bioactive compounds) was crystallized with solvent to further improve the con-tent of active compounds. The optimal methyl esterification conditions were determined by single factor ex-periment as follows:reaction temperature 70℃, reaction time 3 h, mass ratio of methanol to oil 3∶10, dos-age of concentrated sulfuric acid 3%. Under these conditions, acid value of shea olein reduced from 56. 1 mgKOH/g to 2. 1 mgKOH/g, and the conversion rate of free fatty acids reached 96. 3%. The optimal con-ditions of molecular distillation were obtained by single factor experiment as follows:feed rate 2 mL/min, scraper speed 250 r/min, distillation temperature 150℃, vacuum degree 0. 1 Pa. Under these conditions, the recovery of the bioactive compounds in heavy fraction was 95. 9% and the content of bioactive com-pounds was up to 68. 8%. By the methods of methyl esterification, transesterification, molecular distillation and crystallization, the total content of triterpene and sterol in the product could reach 69. 80%, and the content of bioactive compounds could achieve 83. 18%.